Metabolomic and proteomic insights into carbaryl catabolism by Burkholderia sp. C3 and degradation of ten N-methylcarbamates

Abstract

Burkholderia sp. C3, an efficient polycyclic aromatic hydrocarbon (PAH) degrader, can utilize 9 of the 10 N-methylcarbamate insecticides including carbaryl as a sole source of carbon. Rapid hydrolysis of carbaryl in C3 is followed by slow catabolism of the resulting 1-naphthol. This study focused on metabolomes and proteomes in C3 cells utilizing carbaryl in comparison to those using glucose or nutrient broth. Sixty of the 867 detected proteins were involved in primary metabolism, adaptive sensing and regulation, transport, stress response, and detoxification. Among the 41 proteins expressed in response to carbaryl were formate dehydrogenase, aldehyde-alcohol dehydrogenase and ethanolamine utilization protein involved in one carbon metabolism. Acetate kinase and phasin were 2 of the 19 proteins that were not detected in carbaryl-supported C3 cells, but detected in glucose-supported C3 cells. Down-production of phasin and polyhydroxyalkanoates in carbaryl-supported C3 cells suggests insufficient carbon sources and lower levels of primary metabolites to maintain an ordinary level of metabolism. Differential metabolomes (approximately 196 identified polar metabolites) showed up-production of metabolites in pentose phosphate pathways and metabolisms of cysteine, cystine and some other amino acids, disaccharides and nicotinate, in contract to down-production of most of the other amino acids and hexoses. The proteomic and metabolomic analyses showed that carbaryl-supported C3 cells experienced strong toxic effects, oxidative stresses, DNA/RNA damages and carbon nutrient deficiency.

Bioremediation of pesticide-contaminated water resources: the challenge of low concentrations

“…The isolated mutants lost their activity towards lactones, but showed increased affinity towards a number of organophosphate pesticides [43 ]. In a separate example, a bacterium that metabolizes polycyclic aromatic hydrocarbons was used to metabolize the carbaryl group of N-methylcarbamate pesticides [44]. Another emerging approach is to expand the metabolic capacity of pesticide degraders.…”

Mechanism study of alachlor biodegradation by Paecilomyces marquandii with proteomic and metabolomic methods

“…Proteomics and metabolomics are modern and convenient tools, which have been successfully applied to gain a better understanding of the biodegradation mechanisms in different types of organisms e.g., bacterial biodegradation of insecticide N-methylcarbamate by Burkholderia sp. C3 [9] or phenanthrene by Sinorhizobium sp. C4 [10]; fungal degradation of nonylphenol by Metarhizium robertsii [11]; Mucor plumbeus response to pentachlorophenol [12]; or benzoic acid removal by Phanerochaete chrysosporium [13].…”

Insights into functional and evolutionary analysis of carbaryl metabolic pathway from Pseudomonas sp. strain C5pp

“…The insecticidal activity is due to the ester bond which inhibits acetylcholine esterase competitively8. Microorganisms have been reported to utilize carbaryl910111213141516, and the complete pathway has been demonstrated at the functional level in soil isolates, Pseudomonas sp. strains C4, C5 and C615.…”