Metabolomic-based clinical studies and murine models for acute pancreatitis disease: A review

Peng, Yang; Hong, Jiwon; Raftery, Daniel; Xia, Qing; Du, Dan

doi:10.1016/j.bbadis.2021.166123

Cited by 21 publications

(16 citation statements)

References 170 publications

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“…Self-confinement is a characteristic feature of MAP patients who recover with minimal treatment, implying that there are some endogenous protective mechanisms of AP. Systemic metabolism is commonly disrupted during AP, 11 but little is known about whether the altered endogenous metabolites participate in the development of AP. Compelling evidence has established a central role of pancreatic lipase-mediated adipose lipolysis in aggravating pancreatic damage and organ failure.…”

Section: Discussionmentioning

confidence: 99%

“… 8 , 9 Liver injury is common albeit overlooked in both experimental and human AP where Kupffer cells may act as the amplifier that translates pancreatic injury into a systemic inflammatory cascade. 10 Despite some endogenous metabolites are released during AP, 11 how liver metabolites and metabolic processes affect pancreatic and systemic inflammatory responses and vice versa remains elusive.…”

Section: Introductionmentioning

confidence: 99%

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Ketogenesis acts as an endogenous protective programme to restrain inflammatory macrophage activation during acute pancreatitis

Zhang

Shi

et al. 2022

eBioMedicine

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ketogenesis acts as an endogenous protective programme to restrain inflammatory macrophage activation during acute pancreatitis

Zhang

Shi

et al. 2022

eBioMedicine

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“…One milliliter of the supernatant was taken in a glass injection vial for metabolomic analysis. The metabolites were analyzed using the Agilent 1260 SL LC-6520 Quadrupole-Time of Flight (Q-TOF) MS system (Agilent Technologies, Santa Clara, CA, USA) [77][78][79][80].…”

Section: Metabolomic Analysis Of Tubifex By Lc-q-tofmentioning

confidence: 99%

Effects of Dufulin on Oxidative Stress and Metabolomic Profile of Tubifex

Zhu

Yang

et al. 2021

Metabolites

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Dufulin is a highly effective antiviral pesticide used in plants. In this study, a seven-day experiment was conducted to evaluate the effects of Dufulin at five different concentrations (1 × 10−4, 1 × 10−3, 1 × 10−2, 0.1, and 1 mg/L) on Tubifex. LC-MS-based metabolome analysis detected a total of 5356 features in positive and 9110 features in negative, of which 41 showed significant changes and were identified as differential metabolites. Four metabolic pathways were selected for further study. Detailed analysis revealed that Dufulin exposure affected the urea cycle of Tubifex, probably via argininosuccinate lyase (ASL) inhibition. It also affected the fatty acid metabolism, leading to changes in the concentration of free fatty acids in Tubifex. Furthermore, the changes in metabolites after exposure to Dufulin at 1 × 10−2 mg/L were different from those at the other concentrations.

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“… 25 Several studies have investigated application of metabolomics to the diagnosis and differentiation among major types of pancreatitis in humans. 26 Fewer metabolomic studies have been carried out in veterinary medicine 27 , 28 and only a few of them in cats. These studies focused on feline metabolome in DM, 29 , 30 metabolic syndrome, 31 inflammatory bowel disease and alimentary small cell lymphoma, 32 mammary carcinoma, 33 and chronic kidney disease.…”

Section: Introductionmentioning

confidence: 99%

“…Over the last decade, quantifying large sets of metabolites in various biological fluids, referred to as metabolomics, has gained in popularity as a potentially useful method of diagnosing and monitoring many conditions in human medicine, 24 including pancreatic diseases 25 . Several studies have investigated application of metabolomics to the diagnosis and differentiation among major types of pancreatitis in humans 26 . Fewer metabolomic studies have been carried out in veterinary medicine 27,28 and only a few of them in cats.…”

Section: Introductionmentioning

confidence: 99%

Correlation between metabolomic profile constituents and feline pancreatic lipase immunoreactivity

Krasztel

Czopowicz

Szaluś‐Jordanow

et al. 2022

Veterinary Internal Medicne

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Background Feline pancreatic lipase immunoreactivity (fPLI) is commonly used to diagnose pancreatitis in cats (FP). Untargeted metabolomics has been extensively applied in human and veterinary medicine, but no metabolomic studies regarding FP have been conducted. Objectives To identify metabolites significantly associated with increased fPLI. Animals Forty‐nine client‐owned cats: 11 clinically healthy and 38 with various clinical conditions. Methods Analytical cross‐sectional study with convenience sampling. A panel of 630 metabolites belonging to 26 biochemical classes was quantified in plasma using a commercial metabolomic assay. The correlation between plasma metabolite concentrations and serum fPLI was evaluated using Spearman's rank correlation coefficient (Rs) with Bonferroni correction. Multivariable analysis then was performed to control for glomerular filtration rate, liver damage, and blood glucose concentration. The accuracy of selected metabolites in discriminating between cats with normal (≤3.5 μg/L) and increased (>5.3 μg/L) fPLI was estimated using the area under the receiver operating characteristic curve (AUROC). Results Four hundred and seven of 630 metabolites (64.6%) were quantified in all cats. When controlled for potential confounders only 3 sphingolipids were significantly positively correlated with fPLI: 2 cerebrosides: HexCer(d18:1/24:0); (Rs = .56), and HexCer(d18:1/24:1); (Rs = .58) and 1 sphingomyelin: SM C18:0 (Rs = .55). Their AUROCs in identifying cats with increased fPLI were 82% (95% confidence interval [CI 95%], 70%‐94%), 84% (CI 95%, 72%‐96%), and 78% (CI 95%, 65%‐92%), respectively. Conclusions and Clinical Importance Selected sphingolipids are moderately positively correlated with fPLI and appear to have fair to moderate diagnostic accuracy in discriminating between cats with normal and increased fPLI.

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Metabolomic-based clinical studies and murine models for acute pancreatitis disease: A review

Cited by 21 publications

References 170 publications

Ketogenesis acts as an endogenous protective programme to restrain inflammatory macrophage activation during acute pancreatitis

Ketogenesis acts as an endogenous protective programme to restrain inflammatory macrophage activation during acute pancreatitis

Effects of Dufulin on Oxidative Stress and Metabolomic Profile of Tubifex

Correlation between metabolomic profile constituents and feline pancreatic lipase immunoreactivity

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