Metabolomic compounds identified in Piriformospora indica-colonized Chinese cabbage roots delineate symbiotic functions of the interaction

Hua, Mo Da-Sang; Kumar, Rajendran Senthil; Shyur, Lie‐Fen; Cheng, Yao; Zhi-hong, Tian; Oelmüller, Ralf; Yeh, Kai‐Wun

doi:10.1038/s41598-017-08715-2

Cited by 54 publications

(34 citation statements)

References 68 publications

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“…Such a variety of roles at the plant‐microorganisms interface points to the centrality of GABA in interkingdom signalling. In agreement with this notion, GABA was shown to be involved in coordinating metabolic balances in rhizobia‐legume symbiosis (Nasr et al, ; Sulieman & Schulze, ) and symbiosis with mutualistic microbes such as Piriformospora indica (Hua, Kumar, Shyur, & Cheng, ).…”

Section: A Molecular Swiss Knife: Gaba Functions In Relation To Pathomentioning

confidence: 72%

γ‐Aminobutyric acid and related amino acids in plant immune responses: Emerging mechanisms of action

Tarkowski

Signorelli

Höfte

2020

Plant Cell & Environment

100

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The entanglement between primary metabolism regulation and stress responses is a puzzling and fascinating theme in plant sciences. Among the major metabolites found in plants, γ-aminobutyric acid (GABA) fulfils important roles in connecting C and N metabolic fluxes through the GABA shunt. Activation of GABA metabolism is known since long to occur in plant tissues following biotic stresses, where GABA appears to have substantially different modes of action towards different categories of pathogens and pests. While it can harm insects thanks to its inhibitory effect on the neuronal transmission, its capacity to modulate the hypersensitive response in attacked host cells was proven to be crucial for host defences in several pathosystems. In this review, we discuss how plants can employ GABA's versatility to effectively deal with all the major biotic stressors, and how GABA can shape plant immune responses against pathogens by modulating reactive oxygen species balance in invaded plant tissues. Finally, we discuss the connections between GABA and other stress-related amino acids such as BABA (β-aminobutyric acid), glutamate and proline. K E Y W O R D SBABA, biotic stress, GABA, glutamate, hypersensitive response, primary metabolism, proline, ROS

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Section: A Molecular Swiss Knife: Gaba Functions In Relation To Pathomentioning

confidence: 72%

γ‐Aminobutyric acid and related amino acids in plant immune responses: Emerging mechanisms of action

Tarkowski

Signorelli

Höfte

2020

Plant Cell & Environment

100

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“…Indeed, by using diverse approaches, including screening Arabidopsis mutants and/or reporter lines and interfering with hormone biosynthesis or transport ( Zhang et al, 2007 ; Ortíz-Castro et al, 2008 ; Bhattacharyya et al, 2015 ; Bitas et al, 2015 ; Garnica-Vergara et al, 2016 ; Sánchez-López et al, 2016a ; Pérez-Flores et al, 2017 ; Li et al, 2018 ) as well as transcriptomics ( Zhang et al, 2007 ; Bhattacharyya et al, 2015 ; Sánchez-López et al, 2016a ) and proteomics ( Kwon et al, 2010 ; Sánchez-López et al, 2016b ), it was shown that mainly auxin and cytokinin production and signaling as well as auxin transport were affected by microbial VOCs, indicating that plants react to these volatiles through highly conserved regulatory mechanisms. Interestingly, plant growth promotion due to physical contact between S. indica and Arabidopsis also appeared to be linked to auxin signaling ( Schäfer et al, 2009 ; Lee et al, 2011 ; Dong et al, 2013 ; Ye et al, 2014 ; Hua et al, 2017 ) and cytokinin perception (through CRE1/AHK2; Vadassery et al, 2008 ).…”

Section: Discussionmentioning

confidence: 99%

Respiratory CO2 Combined With a Blend of Volatiles Emitted by Endophytic Serendipita Strains Strongly Stimulate Growth of Arabidopsis Implicating Auxin and Cytokinin Signaling

et al. 2020

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Rhizospheric microorganisms can alter plant physiology and morphology in many different ways including through the emission of volatile organic compounds (VOCs). Here we demonstrate that VOCs from beneficial root endophytic Serendipita spp. are able to improve the performance of in vitro grown Arabidopsis seedlings, with an up to 9.3-fold increase in plant biomass. Additional changes in VOC-exposed plants comprised petiole elongation, epidermal cell and leaf area expansion, extension of the lateral root system, enhanced maximum quantum efficiency of photosystem II (F v /F m ), and accumulation of high levels of anthocyanin. Notwithstanding that the magnitude of the effects was highly dependent on the test system and cultivation medium, the volatile blends of each of the examined strains, including the references S. indica and S. williamsii , exhibited comparable plant growth-promoting activities. By combining different approaches, we provide strong evidence that not only fungal respiratory CO 2 accumulating in the headspace, but also other volatile compounds contribute to the observed plant responses. Volatile profiling identified methyl benzoate as the most abundant fungal VOC, released especially by Serendipita cultures that elicit plant growth promotion. However, under our experimental conditions, application of methyl benzoate as a sole volatile did not affect plant performance, suggesting that other compounds are involved or that the mixture of VOCs, rather than single molecules, accounts for the strong plant responses. Using Arabidopsis mutant and reporter lines in some of the major plant hormone signal transduction pathways further revealed the involvement of auxin and cytokinin signaling in Serendipita VOC-induced plant growth modulation. Although we are still far from translating the current knowledge into the implementation of Serendipita VOCs as biofertilizers and phytostimulants, volatile production is a novel mechanism by which sebacinoid fungi can trigger and control biological processes in plants, which might offer opportunities to address agricultural and environmental problems in the future.

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“…However, P. indica mutants with reduced IAA levels due to the silencing of tryptophan aminotransferase ( piTam1 ) showed an average growth‐promoting effect on barley but reduced colonization of roots during the biotrophy stage, suggesting that auxin might act as a signal for communication with host plants (Hilbert et al, ). In addition, an exudate fraction from P. indica , but not auxin, stimulates the growth of Chinese cabbage (Hua et al, ). In the present study, we found that expression of the IAA synthesis gene in F. oryzae was not significantly upregulated at 7 days after cocultivation when F. oryzae had already stimulated root hair growth.…”

Section: Discussionmentioning

confidence: 99%

Endophytic fungus Falciphora oryzae promotes lateral root growth by producing indole derivatives after sensing plant signals

Sun

Wang

et al. 2019

Plant Cell & Environment

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The endophytic fungus Falciphora oryzae was initially isolated from wild rice (Oryza granulata) and colonizes many crop species and promotes plant growth. However, the molecular mechanisms underlying F. oryzae‐mediated growth promotion are still unknown. We found that F. oryzae was able to colonize Arabidopsis thaliana. The most dramatic change after F. oryzae inoculation was observed in the root architecture, as evidenced by increased lateral root growth but reduced primary root length, similar to the effect of auxin, a significant plant growth hormone. The expression of genes responsible for auxin biosynthesis, transport, and signalling was regulated in Arabidopsis roots after F. oryzae cocultivation. Indole derivatives were detected at significantly higher levels in liquid media after cocultivation compared with separate cultivation of Arabidopsis and F. oryzae. Consistently, the expression of indole biosynthetic genes was highly upregulated in F. oryzae upon treatment with Arabidopsis exudates. Global analysis of Arabidopsis gene expression at the early stage after F. oryzae cocultivation suggested that signals were exchanged to initiate Arabidopsis–F. oryzae interactions. All these results suggest that signalling molecules from Arabidopsis roots are perceived by F. oryzae and induce the biosynthesis of indole derivatives in F. oryzae, consequently stimulating Arabidopsis lateral root growth.

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Metabolomic compounds identified in Piriformospora indica-colonized Chinese cabbage roots delineate symbiotic functions of the interaction

Cited by 54 publications

References 68 publications

γ‐Aminobutyric acid and related amino acids in plant immune responses: Emerging mechanisms of action

γ‐Aminobutyric acid and related amino acids in plant immune responses: Emerging mechanisms of action

Respiratory CO2 Combined With a Blend of Volatiles Emitted by Endophytic Serendipita Strains Strongly Stimulate Growth of Arabidopsis Implicating Auxin and Cytokinin Signaling

Endophytic fungus Falciphora oryzae promotes lateral root growth by producing indole derivatives after sensing plant signals

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