Metabolomic investigation of Mytilus galloprovincialis (Lamarck 1819) caged in aquatic environments

Fasulo, Salvatore; Iacono, F.; Cappello, Tiziana; Corsaro, Carmelo; Maisano, Maria; D'Agata, Alessia; Giannetto, Alessia; Domenico, Elena De; Parrino, Vincenzo; Paro, Giuseppe Lo; Mauceri, Angela

doi:10.1016/j.ecoenv.2012.07.001

Cited by 127 publications

(60 citation statements)

References 45 publications

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“…With the rapid development of -omic techniques including genomics, transcriptomics, proteomics and metabolomics, researchers can focus on a global profile of one type of molecules such as genes, proteins and metabolites and their alterations with high-throughput analyses, which offer great potential in unraveling toxicological effects and mechanisms of environmental contaminants (Weckwerth, 2011;Fasulo et al, 2012;Cappello et al, 2013a,b;Ji et al, 2013). Among these approaches, proteomics is a large-scale study of proteins encoded by the given genome in an organism (Anderson and Anderson, 1998).…”

Section: Discussionmentioning

confidence: 99%

iTRAQ-based quantitative proteomic analyses on the gender-specific responses in mussel Mytilus galloprovincialis to tetrabromobisphenol A

Wei

et al. 2014

Aquatic Toxicology

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a b s t r a c tTetrabromobisphenol A (TBBPA) accounts for the largest production of brominated flame-retardants (BFRs) along the Laizhou Bay in China and is the most widely used BFR in industrial products. It can induce diverse toxicities including hepatotoxicity, nephrotoxicity, neurotoxicity and endocrine disrupting effects in mammalian and fish models. In this work, we applied iTRAQ-based proteomics to investigate the gender-specific responses in mussel Mytilus galloprovincialis to TBBPA. Thirty-one proteins were differentially expressed in hepatopancreas between male and female mussels, which clearly indicated the biological differences between male and female mussels at the protein level. After exposure of TBBPA (18.4 nmol/L) for one month, a total of 60 proteins were differentially expressed in response to the TBBPA treatment in mussel hepatopancreas, among which 33 and 29 proteins were significantly altered in TBBPA-treated male and female mussel samples, respectively. Only two of the 60 proteins were commonly altered in both male and female mussel samples exposed to TBBPA. Based on KEGG analysis, these differentially expressed proteins of TBBPA-induced effects were assigned to several groups, including cytoskeleton, reproduction and development, metabolism, signal transduction, gene expression, stress response and apoptosis. Overall, results indicated that TBBPA exposure could induce apoptosis, oxidative and immune stresses and disruption in energy, protein and lipid metabolisms in both male and female mussels with different mechanisms. This work suggested that the gender differences should be considered in ecotoxicoproteomics.

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Section: Discussionmentioning

confidence: 99%

iTRAQ-based quantitative proteomic analyses on the gender-specific responses in mussel Mytilus galloprovincialis to tetrabromobisphenol A

Wei

et al. 2014

Aquatic Toxicology

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“…Among these -omics techniques, metabolomics focuses on the whole set of metabolites (b 1000 Da) in organisms (Jones et al, 2008;Viant et al, 2003). A comparative analysis on the metabolic profiles can detect the metabolic perturbations in organisms caused by environmental pollution, which may interpret the biological effects of environmental pollution (Fasulo et al, 2012;Kwon et al, 2012;Cappello et al, 2013). Previously, Kwon et al (2012) successfully applied nuclear magnetic resonance (NMR)-based metabolomics to study the biological effects of metal pollution on mussels (Mytilus edulis) collected from the metal-polluted area, Onsan Bay.…”

Section: H Nmrmentioning

confidence: 99%

Metabolic responses to metal pollution in shrimp Crangon affinis from the sites along the Laizhou Bay in the Bohai Sea

Zhao

et al. 2016

Marine Pollution Bulletin

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a b s t r a c t a r t i c l e i n f oMarine environment in the Laizhou Bay is potentially contaminated by metals from industrial discharges. In this study, metal concentrations in shrimps Crangon affinis indicated that two typical sites (S6283 and S5283) close to Longkou and Zhaoyuan cities along the Laizhou Bay have been contaminated by metals, including Cd, As, Cu, Ni, Co, and Mn. In particular, Cd and As were the main metal contaminants in S6283. In S5283, however, Cu was the most important metal contaminant. The metabolic responses in the shrimps indicated that the metal pollution in S6283 and S5283 induced disturbances in osmotic regulation and energy metabolism and reduced anaerobiosis, lipid metabolism, and muscle movement. However, alteration in the levels of dimethylglycine, dimethylamine, arginine, betaine, and glutamine indicated that the metal pollution in S5283 induced osmotic stress through different pathways compared to that in S6283. In addition, dimethylamine might be the biomarker of Cu in shrimp C. affinis.

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“…Metabolomics is a newly establishedomics technique that focuses on all the small molecular metabolites (b1000 Da) in organisms (Jones et al, 2008;Viant et al, 2003). A comparative analysis on the metabolome profiles in organisms under environmental stress can provide metabolic responses induced by environmental stressors (Fasulo et al, 2012;Kwon et al, 2012;Cappello et al, 2013). In a previous study, Kwon et al (2012) successfully applied NMR-based metabolomics to investigate the biological effects of metal pollution in marine mussels (Mytilus edulis) sampled from a metal-polluted area (Onsan Bay).…”

Section: H Nmrmentioning

confidence: 99%

Impact of metal pollution on shrimp Crangon affinis by NMR-based metabolomics

Wang

et al. 2016

Marine Pollution Bulletin

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a b s t r a c t a r t i c l e i n f oBoth cadmium and arsenic are the important metal/metalloid pollutants in the Bohai Sea. In this work, we sampled the dominant species, shrimp Crangon affinis, from three sites, the Middle of the Bohai Sea (MBS), the Yellow River Estuary (YRE) and the Laizhou Bay (LZB) along the Bohai Sea. The concentrations of metals/metalloids in shrimps C. affinis indicated that the YRE site was polluted by Cd and Pb, while the LZB site was contaminated by As. The metabolic differences between shrimps C. affinis from the reference site (MBS) and metal-pollution sites (YRE and LZB) were characterized using NMR-based metabolomics. Results indicated that the metal pollutions in YRE and LZB induced disturbances in osmotic regulation and energy metabolism via different metabolic pathways. In addition, a combination of alanine and arginine might be the biomarker of Cd contamination, while BCAAs and tyrosine could be the biomarkers of arsenic contamination in C. affinis.

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Metabolomic investigation of Mytilus galloprovincialis (Lamarck 1819) caged in aquatic environments

Cited by 127 publications

References 45 publications

iTRAQ-based quantitative proteomic analyses on the gender-specific responses in mussel Mytilus galloprovincialis to tetrabromobisphenol A

iTRAQ-based quantitative proteomic analyses on the gender-specific responses in mussel Mytilus galloprovincialis to tetrabromobisphenol A

Metabolic responses to metal pollution in shrimp Crangon affinis from the sites along the Laizhou Bay in the Bohai Sea

Impact of metal pollution on shrimp Crangon affinis by NMR-based metabolomics

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