Metabolomic Profiling of Plasma from Melioidosis Patients Using UHPLC-QTOF MS Reveals Novel Biomarkers for Diagnosis

Lau, Susanna K.P.; Lee, Kim Chung; Lo, George C.S.; Ding, Vanessa S.Y.; Chow, Wang Ngai; Ke, Tony Y.H.; Curreem, Shirly O. T.; To, Kelvin Kai-Wang; Ho, Deborah Tip Yin; Sridhar, Siddharth; Wong, Sally C.Y.; Chan, Jasper Fuk‐Woo; Hung, Ivan F. N.; Sze, Kong Hung; Lam, Ching Wan; Yuen, Kwok‐Yung; Woo, Patrick C. Y.

doi:10.3390/ijms17030307

Cited by 19 publications

(12 citation statements)

References 51 publications

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“…LysoPE which is a lyso-type phospholipid, is a metabolic product of phosphatidylethanolamine (PE, a minor constituent of cell membranes) generated by phospholipase A2 [ 33 ]. LysoPE has been shown to mobilize intracellular Ca 2+ through G-protein-coupled receptor (GPCR) in some cells types, such as MDA-MB-231 breast cancer cells, skov3 human ovarian cancer cells and human SH-SY5Y neuroblastoma cells [ 34 – 36 ].…”

Section: Discussionmentioning

confidence: 99%

Lipidomic analysis of serum samples from migraine patients

Ren¹,

Liu²,

Zhou³

et al. 2018

Lipids Health Dis

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BackgroundMigraine is a prevalent, disabling type of primary headache disorder associated with a high socioeconomic burden. The clinical management of migraine is challenging. This study was to identify potential serum lipidomic biomarkers of migraine.MethodsThe serum lipidomic profile of migraine sufferers was compared with healthy individuals using Liquid Chromatography coupled to Mass Spectrometry (LC-MS). Volcano plot analysis by Student’s t-test was performed to identify the differential metabolites. Receiver operating characteristic (ROC) curves were constructed and the area under ROC curves (AUC) was calculated to evaluate whether the metabolites could be efficiently exploited for constructing a sensitive biomarker of migraine.ResultsA total of 29 serum metabolites from 4 classes of lipids including acylcarnitines, non-alpha-hydroxy-sphingosine ceramides (Cer_NSs), lysophosphatidylcholines (lysoPCs) and lysophosphatidylethanolamines (lysoPEs) were significantly different in migraine patients and controls. Of note, Cer_NSs were significantly elevated and lysoPEs were significantly decreased in migraine patients. LysoPE 18:1, lysoPE 18:2 and lysoPE 22:5 were found to be decreased in both positive and negative ion mode. Moreover, except for lysoPC 20:0, other lysoPCs were decreased in migraine patients. ROC curve analysis indicated that lysoPC 16:0 and lysoPC 20:0 are potential sensitive and specific biomarkers for migraine.ConclusionLysoPC 16:0 and lysoPC 20:0 may be potential biomarkers for migraine. We suggest therapeutic management of these metabolites may be helpful in the prevention and treatment of migraine.Electronic supplementary materialThe online version of this article (10.1186/s12944-018-0665-0) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Lipidomic analysis of serum samples from migraine patients

Ren¹,

Liu²,

Zhou³

et al. 2018

Lipids Health Dis

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“…In our study, sphingomyelins were increased only in the serum of vaccinated horses. Sphingomyelin levels have previously been shown to be elevated in patients with pneumonia [ 37 ], and melioidosis [ 53 ] while they are unaltered in several bacteremic conditions [ 53 ] and decreased in AIDS patients [ 38 ]. Our data indicate that the immune response induced by vaccination has a more dramatic effect than leptospiral infection on sphingomyelin synthesis in horses.…”

Section: Discussionmentioning

confidence: 99%

Lipidomic analysis of immune activation in equine leptospirosis and Leptospira-vaccinated horses

et al. 2018

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Currently available diagnostic assays for leptospirosis cannot differentiate vaccine from infection serum antibody. Several leptospiral proteins that are upregulated during infection have been described, but their utility as a diagnostic marker is still unclear. In this study, we undertook a lipidomics approach to determine if there are any differences in the serum lipid profiles of horses naturally infected with pathogenic Leptospira spp. and horses vaccinated against a commercially available bacterin. Utilizing a high-resolution mass spectrometry serum lipidomics analytical platform, we demonstrate that cyclic phosphatidic acids, diacylglycerols, and hydroperoxide oxidation products of choline plasmalogens are elevated in the serum of naturally infected as well as vaccinated horses. Other lipids of interest were triacylglycerols that were only elevated in the serum of infected horses and sphingomyelins that were increased only in the serum of vaccinated horses. This is the first report looking at the equine serum lipidome during leptospiral infection and vaccination.

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“…43 LysoPE (0:0/ 20:4(8Z,11Z,14Z,17Z)) and LysoPE (16:0/0:0) are the metabolic products of phosphatidylethanolamine (PE). 44 Both PC and PE are structural components of cell membranes, 43,44 and they can be generated by phospholipase A2. 44,45 Another pathway for LysoPC formation occurs by the transfer of one fatty acid of PC to cholesterol by lecithin-cholesterol acyltransferase (LCAT), which is an enzyme that converts free cholesterol into cholesteryl ester.…”

Section: Discussionmentioning

confidence: 99%