Metabolomics Analysis of Pot-Pollen from Three Species of Australian Stingless Bees (Meliponini)

Massaro, Carmelina Flavia; Villa, Tommaso Francesco; Hauxwell, Caroline

doi:10.1007/978-3-319-61839-5_29

Cited by 3 publications

(2 citation statements)

References 32 publications

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“…Notably, however, PAs have been reported in the propolis of Brazilian Scaptotrigona postica [ 13 , 14 , 15 , 16 ] but not in stingless bee honey. PAs were previously searched for in the pot-pollen of T. carbonaria , T. hockingsi and Austroplebeia australis in the mass range m / z 398.2184 – m / z 496.3402, but no PAs were detected [ 17 ], using HPLC combined with a diode array detector and Orbitrap high-resolution mass spectrometry.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Pyrrolizidine Alkaloids in Stingless Bee Honey and Identification of a Botanical Source as Ageratum conyzoides

Hungerford,

Zawawi,

Zhu

et al. 2024

Toxins

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Stingless bee honeys (SBHs) from Australian and Malaysian species were analysed using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) for the presence of pyrrolizidine alkaloids (PAs) and the corresponding N-oxides (PANOs) due to the potential for such hepatotoxic alkaloids to contaminate honey as a result of bees foraging on plants containing these alkaloids. Low levels of alkaloids were found in these SBHs when assessed against certified PA standards in targeted analysis. However, certain isomers were identified using untargeted analysis in a subset of honeys of Heterotrigona itama which resulted in the identification of a PA weed species (Ageratum conyzoides) near the hives. The evaluation of this weed provided a PA profile matching that of the SBH of H. itama produced nearby, and included supinine, supinine N-oxide (or isomers) and acetylated derivatives. These PAs lacking a hydroxyl group at C7 are thought to be less hepatoxic. However, high levels were also observed in SBH (and in A. conyzoides) of a potentially more toxic diester PA corresponding to an echimidine isomer. Intermedine, the C7 hydroxy equivalent of supinine, was also observed. Species differences in nectar collection were evident as the same alkaloids were not identified in SBH of G. thoracica from the same location. This study highlights that not all PAs and PANOs are identified using available standards in targeted analyses and confirms the need for producers of all types of honey to be aware of nearby potential PA sources, particularly weeds.

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Section: Introductionmentioning

confidence: 99%

Analysis of Pyrrolizidine Alkaloids in Stingless Bee Honey and Identification of a Botanical Source as Ageratum conyzoides

Hungerford,

Zawawi,

Zhu

et al. 2024

Toxins

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“…Stingless bees can be found in places with tropical or subtropical climate, such as Malaysia (Flavia Massaro et al, 2018). Honey produced by stingless bees is known as Meliponine honey, or stingless bee honey (SBH), and also Kelulut honey in Malaysia (Zulkhairi Amin et al, 2018).…”

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confidence: 99%

Physicochemical analysis and adulteration detection in Malaysia stingless bee honey using a handheld near‐infrared spectrometer

Tan

Pui

Solihin

et al. 2021

J. Food Process. Preserv.

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Increasing food demand creates both opportunities and challenges in food safety (Food Safety, 2020). Food safety covers different aspects, which include the risk from food that can cause harm to consumer in terms of health and acute injury to its consumer (Important Food Issues, 2003). When the supply of food cannot fulfill the food demand of consumers, it leads to food fraud occurrence (Abu Bakar et al., 2017). Food fraud is the act of intentionally changing, misrepresenting, mislabeling, removing, or altering with any food product throughout the farm-to-table food supply chain (Spink, Bedard, et al., 2019). A series of incidents in purposeful food adulteration have urged its assessment, choosing countermeasures and control systems by the public and government or local authorities (Spink, Elliott, et al., 2019). The food producers and food handlers take greater responsibility and roles in ensuring food safety and preventing food fraud. Besides that, food safety plays a role in enforcing food laws, for public protection.

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Effect of superficial air velocity on the fluidized bed drying performance of stingless bee pot-pollen

Halim

Basrawi

Faizal

et al. 2020

IOP Conf. Ser.: Mater. Sci. Eng.

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Stingless bees or “kelulut” also produce pot-pollen apart from honey. The pot-pollen is mixed with honey and bee secretion before stored in cerumen pots. It has high nutritive value and medicinal benefits. Pot-pollens are often neglected by the beekeepers due to difficulty in storing and preserving them due to high moisture content. Hence, fluidized bed dryer is proposed as a suitable method to dry and enable convenient storage and preservation of the pot-pollen. Pot-pollen sample of initial moisture content 30.5% is dried at three superficial air velocities, 1.0 m/s, 1.5 m/s, and 2.0 m/s for 30 minutes. Fluidized bed drying has managed to decrease the moisture content down to 23%, 20.5%, and 18.5%, respectively. Higher superficial air velocity lead to higher drying rate in of pot-pollen. Hence, using a fluidized bed dryer to dry stingless bee pot-pollen is a promising method for preserving them. Subsequently, the dried pot-pollen can be easily commercialized in the future.

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Metabolomics Analysis of Pot-Pollen from Three Species of Australian Stingless Bees (Meliponini)

Cited by 3 publications

References 32 publications

Analysis of Pyrrolizidine Alkaloids in Stingless Bee Honey and Identification of a Botanical Source as Ageratum conyzoides

Analysis of Pyrrolizidine Alkaloids in Stingless Bee Honey and Identification of a Botanical Source as Ageratum conyzoides

Physicochemical analysis and adulteration detection in Malaysia stingless bee honey using a handheld near‐infrared spectrometer

Effect of superficial air velocity on the fluidized bed drying performance of stingless bee pot-pollen

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