Metabolomics and biochemical analyses revealed metabolites important for the antioxidant properties of purple glutinous rice

Xiong, Qiangqiang; Sun, Changhui; Li, Ao; Zhang, Jiao; Shi, Qiang; Zhang, Yanhong; Hu, Jinlong; Zhou, Nianbing; Wei, Haiyan; Liu, Bingliang; Zhang, Hongcheng; Jun, Zhu

doi:10.1016/j.foodchem.2022.133080

Cited by 33 publications

(24 citation statements)

References 19 publications

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“…Metabolomics is currently being utilized to qualitatively and quantitatively evaluate all metabolites to offer an advanced assessment technique of the cellular state. The HPLC-MS/MS-based metabolomics are widely used to profile metabolites involved in the physiological responses to environmental challenges in plants ( Liu et al, 2021 ; Sun et al, 2021 ; Xiong et al, 2022 ). In the present study, we utilized a widely targeted metabolomics approach using the UPLC-MS/MS analytical platform to analyze the metabolite changes of P. nutans under cold stress ( Figure 2 ).…”

Section: Discussionmentioning

confidence: 99%

Metabolic profiling and gene expression analyses provide insights into cold adaptation of an Antarctic moss Pohlia nutans

Liu

Fang

et al. 2022

Front. Plant Sci.

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Antarctica is the coldest, driest, and most windy continent on earth. The major terrestrial vegetation consists of cryptogams (mosses and lichens) and two vascular plant species. However, the molecular mechanism of cold tolerance and relevant regulatory networks were largely unknown in these Antarctic plants. Here, we investigated the global alterations in metabolites and regulatory pathways of an Antarctic moss (Pohlia nutans) under cold stress using an integrated multi-omics approach. We found that proline content and several antioxidant enzyme activities were significantly increased in P. nutans under cold stress, but the contents of chlorophyll and total flavonoids were markedly decreased. A total of 559 metabolites were detected using ultra high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS). We observed 39 and 71 differentially changed metabolites (DCMs) after 24 h and 60 h cold stress, indicating that several major pathways were differentially activated for producing fatty acids, alkaloids, flavonoids, terpenoids, and phenolic acids. In addition, the quantitative transcriptome sequencing was conducted to uncover the global transcriptional profiles of P. nutans under cold stress. The representative differentially expressed genes (DEGs) were identified and summarized to the function including Ca2+ signaling, ABA signaling, jasmonate signaling, fatty acids biosynthesis, flavonoid biosynthesis, and other biological processes. The integrated dataset analyses of metabolome and transcriptome revealed that jasmonate signaling, auxin signaling, very-long-chain fatty acids and flavonoid biosynthesis pathways might contribute to P. nutans acclimating to cold stress. Overall, these observations provide insight into Antarctic moss adaptations to polar habitats and the impact of global climate change on Antarctic plants.

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Section: Discussionmentioning

confidence: 99%

Metabolic profiling and gene expression analyses provide insights into cold adaptation of an Antarctic moss Pohlia nutans

Liu

Fang

et al. 2022

Front. Plant Sci.

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“…TY871 and TY398 ( Figure 6 H) were rated as national standard high-quality rice grade two. Previous studies have shown that grain metabolites may be one of the central factors affecting the nutritional quality and eating quality of rice [ 17 , 18 , 19 ]. In this study, NEG_M341T662 metabolites were found to be highly correlated with rice quality ( Figure S6 ), and NEG_M341T662 was highly connected to the core genes Os06g0254300 and Os03g0168100 ( Figure 5 ).…”

Section: Discussionmentioning

confidence: 99%

“…Compared with their parents, hybrids may have different allelic expression levels at specific loci, opening new directions for heterosis research [ 15 ]. The transcriptome and metabolome have been widely used in rice grain development and rice quality research [ 16 , 17 ]. Metabolites in rice can broadly reflect the overall metabolic state of rice seeds [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome and Metabolome Analysis Provides Insights into the Heterosis of Yield and Quality Traits in Two Hybrid Rice Varieties (Oryza sativa L.)

Zhou

Sun

et al. 2022

IJMS

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Heterosis is a common biological phenomenon that is useful for breeding superior lines. Using heterosis to increase the yield and quality of crops is one of the main achievements of modern agricultural science. In this study, we analysed the transcriptome and metabolome of two three-line hybrid rice varieties, Taiyou 871 (TY871), and Taiyou 398 (TY398) and the parental grain endosperm using RNA-seq (three biological repeats per variety) and untargeted metabolomic (six biological repeats per variety) methods. TY871 and TY398 showed specific heterosis in yield and quality. Transcriptome analysis of the hybrids revealed 638 to 4059 differentially expressed genes in the grain when compared to the parents. Metabolome analysis of the hybrids revealed 657 to 3714 differential grain metabolites when compared to the parents. The honeydew1 and grey60 module core genes Os04g0350700 and Os05g0154700 are involved in the regulation of awn development, grain size, and grain number, as well as the regulation of grain length and plant height, respectively. Rice grain length may be an important indicator for improving the quality of three-line hybrid rice. In addition, the rice quality-related metabolite NEG_M341T662 was highly connected to the module core genes Os06g0254300 and Os03g0168100. The functions of Os06g0254300 and Os03g0168100 are EF-hand calcium binding protein and late embroideries absolute protein repeat containing protein, respectively. These genes may play a role in the formation of rice quality. We constructed a gene and metabolite coexpression network, which provides a scientific basis for the utilization of heterosis in producing high-yield and high-quality hybrid rice.

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“…We used the Xiong et al (2022 a, b) technique and used precise weights to measure the samples of head milled rice (50 mg) [ 16 , 17 ] to extract metabolites. The 50 mg head-milled rice sample was accurately weighed into a 2 mL centrifuge tube, and a 6 mm diameter bead was added.…”

Section: Methodsmentioning

confidence: 99%

“…Among them, lipid metabolites are a significant component influencing the TV of rice [ 16 ]. In addition, the important metabolites of purple glutinous rice were analyzed using metabolomics technology, and six metabolites were identified as candidate metabolite biomarkers for assessing the glutinous rice antioxidant capacity [ 17 ]. The regulatory functions of important metabolites in metabolic processes were then defined after an additional analysis of the metabolites related to the nutrients of purple glutinous rice [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Rice Quality-Related Metabolites and the Regulatory Roles of Key Metabolites in Metabolic Pathways of High-Quality Semi-Glutinous japonica Rice Varieties

Jun

Sun

et al. 2022

Foods

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We explored the related metabolites produced by different quality semi-glutinous japonica rice varieties and the modulatory role of key metabolites in metabolic mechanisms. In this study, three high-quality edible semi-glutinous rice varieties were employed as investigational materials, the metabolites of the three varieties were detected using LC–MS metabolomics technology, and the rice quality traits of the three rice varieties were determined. The taste value (TV) of Yangnongxiang 28 (YNX28H) was substantially higher than that of Hongyang 5 hao (HY5H) and Nanjing 5718 (NJ5718), and the hardness (HA) of YNX28H was significantly lower than that of HY5H and NJ5718. The HA was significantly negatively correlated with the TV. The highest chalkiness rate (CR) and chalkiness degree (CD) were observed for NJ5718, and the lowest CR and CD were observed for HY5H. HY5H had a substantially lower protein content (PC) than YNX28H and NJ5718 and a markedly higher amylose content (AC) than those two varieties. Overall, 188 differential metabolites (DMs) were recognized between HY5H and NJ5718. A total of 136 DMs were detected between YNX28H and NJ5718, and 198 DMs were recognized between HY5H and YNX28H. The metabolites with a strong correlation with rice quality were mainly associated with amino acid metabolism, lipid metabolism and the citrate cycle. The key metabolites in the metabolic pathway include lipid metabolites (sagittariol, glycerophosphocholine, gamma-eudesmol rhamnoside, goshonoside F1, diosbulbinoside F, and corchorifatty acid F), amino acid metabolites (pantothenic acid, L-serine, L-proline, L-aspartic acid, L-glutamate, L-asparagine, and glutathione) and carbohydrate metabolites (sucrose, levan, D-maltose, and amylose). These key metabolites play important regulatory roles in metabolic mechanisms, providing a theoretical basis for breeding new high-quality edible rice varieties.

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Metabolomics and biochemical analyses revealed metabolites important for the antioxidant properties of purple glutinous rice

Cited by 33 publications

References 19 publications

Metabolic profiling and gene expression analyses provide insights into cold adaptation of an Antarctic moss Pohlia nutans

Metabolic profiling and gene expression analyses provide insights into cold adaptation of an Antarctic moss Pohlia nutans

Transcriptome and Metabolome Analysis Provides Insights into the Heterosis of Yield and Quality Traits in Two Hybrid Rice Varieties (Oryza sativa L.)

Rice Quality-Related Metabolites and the Regulatory Roles of Key Metabolites in Metabolic Pathways of High-Quality Semi-Glutinous japonica Rice Varieties

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