Metabolomics and proteomics reveal the toxicological mechanisms of florfenicol stress on wheat (Triticum aestivum L.) seedlings

Chen, Hanmei; Jin, Jiaojun; Hu, Shuhao; Shen, Luoqin; Zhang, Ping; Li, Zhiheng; Fang, Zhiguo; Liu, Huijun

doi:10.1016/j.jhazmat.2022.130264

Cited by 27 publications

(8 citation statements)

References 51 publications

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“…Under propofol exposure, the levels of citric and isocitric acids were significantly increased in zebrafish, which are indicative of oxidation of propofol in the TCA cycle and impaired mitochondrial enzyme activity. The dysfunction of enzyme function induced inefficient enzymatic reactions, leading to the accumulation of TCA cycle intermediates . The accumulation of metabolites in the TCA cycle can also cause oxidative stress and increased levels of ROS, leading to mitochondrial dysfunction, which is consistent with our findings at the biochemical level.…”

Section: Resultssupporting

confidence: 91%

“…The dysfunction of enzyme function induced inefficient enzymatic reactions, leading to the accumulation of TCA cycle intermediates. 48 The accumulation of metabolites in the TCA cycle can also cause oxidative stress and increased levels of ROS, leading to mitochondrial dysfunction, 49 which is consistent with our findings at the biochemical level. As structural components of DNA and RNA molecules, purine nucleotides are the source of adenosine triphosphate (ATP) and play a crucial role in energy supply, metabolic regulation, and other aspects.…”

Section: Multilayered Molecular Alterationssupporting

confidence: 86%

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Environmentally Relevant Concentrations of Propofol-Induced Metabolic Dysfunction in Zebrafish: Mechanistic Insights into Integrated Hepatic Transcriptomic and Metabolomic Analyses

Jiang,

Li,

Zhang

et al. 2024

ACS EST Water

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Propofol is increasingly perceived as an emerging threat to aquatic ecosystems, although the ecotoxicity and underlying mechanisms of propofol on aquatic life have not yet been clearly established. In this study, zebrafish (Danio rerio) was exposed to different concentrations of propofol (0.008, 0.04, and 0.2 mg•L −1 ) to probe the effect of propofol on functional metabolism and the ecological response mechanism in aquatic organisms. Propofol induced severe hepatic damage, including increased interstitial spaces and nuclear malformations at the tissue level. Reactive oxygen species (ROS) production and lipid peroxidation in zebrafish were induced after 28 days of exposure, while the activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were substantially increased, suggesting that propofol may influence hepatic tissue injury through exacerbating oxidative damage. Exacerbated levels of triglyceride (TG) and high-density lipoprotein cholesterol (HDL-C) were indicative of dysregulated lipid metabolism. Comprehensive transcriptomics and metabolomics analyses showed that propofol mainly changed the glycerophospholipid metabolism, citric acid cycle, and purine metabolism, and the disorders of these pathways can aggravate lipid accumulation and oxidative damage. Overall, this study revealed the mechanisms of hepatotoxicity and metabolic dysfunction of propofol on zebrafish, providing constructive insights for a comprehensive assessment of the toxicity risk posed by propofol to aquatic environmental health.

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Section: Resultssupporting

confidence: 91%

Section: Multilayered Molecular Alterationssupporting

confidence: 86%

Environmentally Relevant Concentrations of Propofol-Induced Metabolic Dysfunction in Zebrafish: Mechanistic Insights into Integrated Hepatic Transcriptomic and Metabolomic Analyses

Jiang,

Li,

Zhang

et al. 2024

ACS EST Water

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“…In the research by Chen et al, metabolomics and proteomics combined analysis showed that florfenicol stress greatly affected the tricarboxylic acid (TCA) cycle in wheat seedlings. Proteomic analysis showed that florfenicol destroyed the photosynthetic pathway, while metabolomic analysis revealed that florfenicol stress stimulated multiple metabolic pathways . The multiomics analysis revealed the harmful effects of Cd on wheat plants.…”

Section: Discussionmentioning

confidence: 99%

“…Proteomic analysis showed that florfenicol destroyed the photosynthetic pathway, while metabolomic analysis revealed that florfenicol stress stimulated multiple metabolic pathways. 50 The multiomics analysis revealed the harmful effects of Cd on wheat plants. Metabolomic data demonstrated the impact of Cd on lipid and amino acid metabolism, and the regulation of glutathione, α-linolenic acid metabolism, and phenylpropanoid biosynthesis pathways by Cd was shown by proteome data.…”

Section: ■ Discussionmentioning

confidence: 99%

Multiomics Explore the Detoxification Mechanism of Nanoselenium and Melatonin on Bensulfuron Methyl in Wheat Plants

Zhou,

Miao,

Dong

et al. 2024

J. Agric. Food Chem.

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Combining nanoselenium (nano-Se) and melatonin (MT) was more effective than treatment alone against abiotic stress. However, their combined application mitigated the toxic effects of bensulfuron methyl, and enhanced wheat growth and metabolism has not been studied. Metabolomics and proteomics revealed that combining nano-Se and MT markedly activated phenylpropanoid biosynthesis pathways, elevating the flavonoid (quercetin by 33.5 and 39.8%) and phenolic acid (vanillic acid by 38.8 and 48.7%) levels in leaves and roots of wheat plants. Interstingly, beneficial rhizosphere bacteria in their combination increased (Oxalobacteraceae, Nocardioidaceae, and Xanthomonadaceae), which positively correlated with the enhancement of soil urease and fluorescein diacetate enzyme activity (27.0 and 26.9%) and the allelopathic substance levels. To summarize, nano-Se and MT mitigate the adverse effects of bensulfuron methyl by facilitating interactions between the phenylpropane metabolism of the plant and the beneficial microbial community. The findings provide a theoretical basis for using nano-Se and MT to remediate herbicidecontaminated soil.

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“…Plants can activate endogenous defense mechanisms in response to ROS-induced oxidative stress. The defense system is mainly composed of antioxidant enzymes and chelates, such as SOD, APX, and POD, which can scavenge free radicals and neutralize intermediates with oxidative toxicity to maintain plants’ homeostasis, thus reducing oxidative damage in plant cells (Chen et al, 2022). In this study, it was observed that the activity of antioxidant enzymes (SOD, APX, and POD) and the contents of GSH and soluble sugar increased significantly with the extension of Cr stress time, which was consistent with the results of previous studies.…”

Section: Discussionmentioning

confidence: 99%

Temporal physiological, transcriptomic and metabolomic analyses revealed molecular mechanism ofCanna indica’s response to Cr stress

Wei

Chen

Yang

et al. 2023

Preprint

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Chromium (Cr) can interfere with plant gene expression, change the content of metabolites and affect plant growth. However, the molecular response mechanism of wetland plants at different time sequences under Cr stress has yet to be fully understood.The results showed that Cr stress increased the activities of superoxide dismutase (SOD), ascorbate peroxidase (APX) and peroxidase (POD), the contents of glutathione (GSH), malondialdehyde (MDA), and oxygen free radical (ROS), and inhibited the biosynthesis of photosynthetic pigments, thus leading to changes in plant growth and biomass. that Cr stress mainly affected 12 metabolic pathways, involving 38 differentially expressed metabolites, including amino acids, phenylpropane, and flavonoids. A total of 16247 differentially expressed genes were identified, among which, at the early stage of stress, C. indica responds to Cr toxicity mainly through galactose, starch and sucrose metabolism. With the extension of stress time, plant hormone signal transduction and MAPK signaling pathway in C. indica in the treatment group were significantly affected. Finally, in the late stage of stress, C. indica co-defuses Cr toxicity by activating its Glutathione metabolism and Phenylpropanoid biosynthesis. In conclusion, this study revealed the molecular response mechanism of C. indica to Cr stress at different times through multi-omics methods.

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Metabolomics and proteomics reveal the toxicological mechanisms of florfenicol stress on wheat (Triticum aestivum L.) seedlings

Cited by 27 publications

References 51 publications

Environmentally Relevant Concentrations of Propofol-Induced Metabolic Dysfunction in Zebrafish: Mechanistic Insights into Integrated Hepatic Transcriptomic and Metabolomic Analyses

Environmentally Relevant Concentrations of Propofol-Induced Metabolic Dysfunction in Zebrafish: Mechanistic Insights into Integrated Hepatic Transcriptomic and Metabolomic Analyses

Multiomics Explore the Detoxification Mechanism of Nanoselenium and Melatonin on Bensulfuron Methyl in Wheat Plants

Temporal physiological, transcriptomic and metabolomic analyses revealed molecular mechanism ofCanna indica’s response to Cr stress

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