Metabolomics: On the way to an integration of biochemistry, analytical chemistry, and informatics

Гончаров, Н. В.; Ukolov, A. I.; Орлова, Т. И.; Migalovskaia, E. D.; Войтенко, Н. Г.

doi:10.1134/s2079086415040027

Cited by 8 publications

(5 citation statements)

References 79 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An ideal biomarker is characterized by a high sensitivity, specificity, and predictive value; it is reproduced in humans of different sexes and ethnic groups, and the procedure for its determination is cost-effective. However, rarely does such a marker stand alone as a single parameter; rather, it is a derivative of several original indicators [212,213]. At the same time, a complex of physiological and biochemical methods combined with an appropriate analytical platform should be relatively simple (low-invasive and noninvasive methods), universal (modular), and flexible (algorithmic).…”

Section: New Methodological Techniques For Identifying Newmentioning

confidence: 99%

“…It is important to emphasize that the methods of metabolomics are not intended to replace the methods of classical biochemistry (spectrophotometry, enzyme immunoassay, etc.). Metabolic profiling provides a unique opportunity to perform diagnosing and monitoring at a qualitatively new level only in conjunction with the methods of biochemical analysis [212].…”

Section: Oxidative Medicine and Cellular Longevitymentioning

confidence: 99%

“…Toxicometabolomics is intended to integrate our understanding of the toxicokinetics of chemicals and the toxicodynamics of physiological and biochemical response to toxic effects, as well as to specify the concepts of homeostasis, stress, and mechanisms of adaptation to the effects of foreign chemical compounds. The result of this integration can be a complete mapping of the "toxicity pathways" and creating a "toxome"-a holistic understanding of the mechanisms of action of substances and the relationship between the dose, time, and consequences of exposure [212,244]. These two scientific directions are almost ready to converge due to development of common algorithmic and computational models and tools.…”

Section: Concluding Remarks and Future Directionsmentioning

confidence: 99%

See 2 more Smart Citations

Markers and Biomarkers of Endothelium: When Something Is Rotten in the State

Гончаров

Nadeev

Jenkins

et al. 2017

Oxidative Medicine and Cellular Longevity

167

115

View full text Add to dashboard Cite

Endothelium is a community of endothelial cells (ECs), which line the blood and lymphatic vessels, thus forming an interface between the tissues and the blood or lympha. This strategic position of endothelium infers its indispensable functional role in controlling vasoregulation, haemostasis, and inflammation. The state of endothelium is simultaneously the cause and effect of many diseases, and this is coupled with modifications of endothelial phenotype represented by markers and with biochemical profile of blood represented by biomarkers. In this paper, we briefly review data on the functional role of endothelium, give definitions of endothelial markers and biomarkers, touch on the methodological approaches for revealing biomarkers, present an implicit role of endothelium in some toxicological mechanistic studies, and survey the role of reactive oxygen species (ROS) in modulation of endothelial status.

show abstract

Section: New Methodological Techniques For Identifying Newmentioning

confidence: 99%

Section: Oxidative Medicine and Cellular Longevitymentioning

confidence: 99%

Section: Concluding Remarks and Future Directionsmentioning

confidence: 99%

See 1 more Smart Citation

Markers and Biomarkers of Endothelium: When Something Is Rotten in the State

Гончаров

Nadeev

Jenkins

et al. 2017

Oxidative Medicine and Cellular Longevity

167

115

View full text Add to dashboard Cite

show abstract

“…Metabolomics using NMR methods give an efficient, with high accuracy and reproducibility screening platform that can classify new antimicrobial drugs by MoA, becoming an excellent complement to HTS evaluation, in this research field have been demonstrated using a metabolome model of Mycobacterium smegmatis the metabolomic chemical structure, biological activity and dose [43]. Finally, with the metabolomics profiles obtained is possible to predict the toxicity response in vitro and in vivo of a specific molecular target or a drug formulation [44].…”

Section: Metabolomics and Antimicrobial Drug Discoverymentioning

confidence: 99%

Metabolomics in Antimicrobial Drug Discovery: The Success of the Chemical Diversity

Bueno¹

2015

J Microb Biochem Technol

View full text Add to dashboard Cite

“…[14][15][16][17][18][19]. At the same time, for practical purposes, it seems promising to search for simple informative indices, for example, based on the ratio of indicators that are in antiphase over a close time interval [20][21][22][23]. For example, among the well-known indices are the De Ritis ratio, the index of atherogenicity, and the body mass index.…”

Section: Introductionmentioning

confidence: 99%

Searching for New Biomarkers to Assess COVID-19 Patients: A Pilot Study

Goncharov,

Avdonin,

Voitenko

et al. 2023

Metabolites

View full text Add to dashboard Cite

During the initial diagnosis of urgent medical conditions, which include acute infectious diseases, it is important to assess the severity of the patient’s clinical state as quickly as possible. Unlike individual biochemical or physiological indicators, derived indices make it possible to better characterize a complex syndrome as a set of symptoms, and therefore quickly take a set of adequate measures. Recently, we reported on novel diagnostic indices containing butyrylcholinesterase (BChE) activity, which is decreased in COVID-19 patients. Also, in these patients, the secretion of von Willebrand factor (vWF) increases, which leads to thrombosis in the microvascular bed. The objective of this study was the determination of the concentration and activity of vWF in patients with COVID-19, and the search for new diagnostic indices. One of the main objectives was to compare the prognostic values of some individual and newly derived indices. Patients with COVID-19 were retrospectively divided into two groups: survivors (n = 77) and deceased (n = 24). According to clinical symptoms and computed tomography (CT) results, the course of disease was predominantly moderate in severity. The first blood sample (first point) was taken upon admission to the hospital, the second sample (second point)—within 4–6 days after admission. Along with the standard spectrum of biochemical indicators, BChE activity (BChEa or BChEb for acetylthiocholin or butyrylthiocholin, respectively), malondialdehyde (MDA), and vWF analysis (its antigen level, AGFW, and its activity, ActWF) were determined and new diagnostic indices were derived. The pooled sensitivity, specificity, and area under the receiver operating curve (AUC), as well as Likelihood ratio (LR) and Odds ratio (OR) were calculated. The level of vWF antigen in the deceased group was 1.5-fold higher than the level in the group of survivors. Indices that include vWF antigen levels are superior to indices using vWF activity. It was found that the index [Urea] × [AGWF] × 1000/(BChEb × [ALB]) had the best discriminatory power to predict COVID-19 mortality (AUC = 0.91 [0.83, 1.00], p < 0.0001; OR = 72.0 [7.5, 689], p = 0.0002). In addition, [Urea] × 1000/(BChEb × [ALB]) was a good predictor of mortality (AUC = 0.95 [0.89, 1.00], p < 0.0001; OR = 31.5 [3.4, 293], p = 0.0024). The index [Urea] × [AGWF] × 1000/(BChEb × [ALB]) was the best predictor of mortality associated with COVID-19 infection, followed by [Urea] × 1000/(BChEb × [ALB]). After validation in a subsequent cohort, these two indices could be recommended for diagnostic laboratories.

show abstract

Metabolomics: On the way to an integration of biochemistry, analytical chemistry, and informatics

Abstract: The review considers the development of a new science-metabolomics. The metabolome is mainly regarded as a set of primarily biochemical parameters, each of which or their ratio can serve as a potential biomarker that increases the sensitivity and/or specificity of diagnostics of diseases.

Cited by 8 publications

References 79 publications

Markers and Biomarkers of Endothelium: When Something Is Rotten in the State

Markers and Biomarkers of Endothelium: When Something Is Rotten in the State

Metabolomics in Antimicrobial Drug Discovery: The Success of the Chemical Diversity

Searching for New Biomarkers to Assess COVID-19 Patients: A Pilot Study

Contact Info

Product

Resources

About