Metabolomics profiling of haloperidol and validation of thromboxane-related signaling in the early development of zebrafish

Reduction of neutral lipid reservoirs, bioconversion and untargeted metabolomics reveal distinct roles for vitamin K isoforms on lipid metabolism

“…The metabolite extraction was performed using two distinct protocols. The general extraction was adapted from Lin et al , 14 as followed: 500 μL of Ripa buffer was added, together with 125 μL of Fmoc-Pro-OH as IS1 (internal standard 1). The samples were homogenized for 2 min (Precellys, Bertin Precellys®24, Montigny-le-Bretonneux, France).…”