Metabolomics Study of Whole-body Vibration on Lipid Metabolism of Skeletal Muscle in Aging Mice

Abstract

Ageing increases the occurrence and development of many diseases. Exercise is believed to be an effective way to improve ageing and skeletal muscle atrophy. However, many elderly people are unable to engage in active exercise. Whole-body vibration is a passive way of moving that is especially suitable for the elderly and people who find it inconvenient to exercise. Metabolomics is the systematic study of metabolic changes in small molecules. In this study, metabolomics studies were performed to investigate the regulatory effect of whole-body vibration on the skeletal muscles of ageing mice. After 12 weeks, we found that whole-body vibration had the most obvious effect on lipid metabolism pathways (such as linoleic acid, α-linolenic acid metabolism, glycerophospholipid metabolism pathways) in skeletal muscle of ageing mice. Through further research we found that whole-body vibration decreased the levels of triglycerides, total cholesterol, low-density lipoprotein cholesterol and very low-density lipoprotein in blood; decreased the lipid deposition in skeletal muscle; decreased the protein expression of monocyte chemoattractant protein-1 and interleukin-6; improved the protein levels of phosphorylated insulin receptor substrate-1, phosphate phosphoinositide 3-kinase and p-AKT; improved the protein levels of klotho; and decreased the protein expression of p53. These findings reveal that whole-body vibration might postpone senility by attenuating lipid deposition and reducing chronic inflammation and the insulin resistance of skeletal muscle.

Immediate Effects of Aerobic Exercise and Whole-Body Vibration on Fat Oxidation, Lipid Mobilization, and Cardiovascular Response in Individuals with Obesity

“…Interestingly, triglyceride levels were lower after completing a WBV (AMAXS AEX 2022, Chase Co., Ltd., Bangkok, Thailand) session. This finding is in agreement with previous studies showing a reduction in triglyceride levels after WBV training in obese and old mice [ 23 , 24 , 25 ]. A vibration stimulus generates force to the muscle via oscillatory motion, leading to muscle activation [ 39 ].…”

“…While this literature points out several health advantages, little is known about the effects of WBV on metabolic-related outcomes. Most previous studies conducted in animal models have evident beneficial effects of WBV on lipid mobilization, such as lowering cholesterol and triglycerides [ 23 , 24 , 25 ]. In addition, a previous study focusing on the acute effects of warm-up with vibration before (AER) on fat oxidation found that an additional WBV during warm-up had greater fat oxidation than warm-up without vibration in obese men [ 26 ].…”

Untargeted Metabolomics Reveals the Effect of Selective Breeding on the Quality of Chicken Meat

“…To acquire knowledge of muscle wasting, Senoo et al adopted a metabolomic method to explore the glycerophospholipid profile of skeletal muscle in a muscular dystrophy model using a denervation murine, and they found an apparent alteration of glycerophospholipids [ 41 ]. Jiang et al reported that whole-body vibration, which is a special exercise for elderly people, had an impact on glycerophospholipid metabolism to reduce lipid deposition in aging muscles [ 42 ].…”

Whole Body Vibration Improves Brain and Musculoskeletal Health by Modulating the Expression of Tissue-Specific Markers: FNDC5 as a Key Regulator of Vibration Adaptations

“…Understanding the biological processes responsible for physiological adaptations to WBV is a key step in the development of appropriate vibratory training protocols tailored to personal needs. Indeed, WBV could be used as a form of passive training for patients unable to exercise, to prevent and/or counteract the cognitive and motor decline associated with sedentariness and the progression of chronic degenerative diseases [ 38 ]. To the best of our knowledge, this is the first experimental study to determine whether exposure to WBV for 3 months can modulate the FNDC5 expression, together with that of tissue-specific markers such as BDNF, myostatin, and COL-1 in the brain, muscle, and bone tissue, respectively.…”