Metabolomics study on revealing the inhibition and metabolic dysregulation in Pseudomonas fluorescens induced by 3-carene

Shu, Huizhen; Zhang, Weimin; Yun, Yong‐Huan; Chen, Weijun; Zhong, Qiuping; Hu, Yueying; Chen, Haiming

doi:10.1016/j.foodchem.2020.127220

Cited by 39 publications

(16 citation statements)

References 42 publications

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“…Respiratory inhibition experiments indicated that limonene exerted a bacteriostatic effect in the TCA cycle of S. aureus, followed by the EMP pathway. TCA cycle plays an important role in cell metabolism, which not only provides energy for life activities, but also links the metabolism of the three major substances of sugar, lipid and protein (Shu, Zhang, Yun, Chen, & Chen, 2020). The inhibition of the TCA cycle will undoubtedly leads to the decrease of dehydrogenase activity, productivity and synthesis of NADH and key metabolites, which will eventually lead to cell death.…”

Section: T-atpase Activitiesmentioning

confidence: 99%

Antimicrobial activity and mechanism of limonene against Staphylococcus aureus

Han

Chen

Sun

2021

Journal of Food Safety

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In this study, inhibitory effect of limonene on Staphylococcus aureus (S. aureus) and its antibacterial mechanism was explored, respectively. The results showed that limonene effectively inhibited bacterial growth at a minimum inhibitory concentration (MIC) of 20 ml/L. Scanning electron microscopy (SEM), the reduction of AKP activity and fluorescence microscope observation confirmed that limonene caused the destruction of the cell morphology and cell wall integrity of S. aureus. The reduction of MFI in fluorescein diacetate staining experiment and leakage of biological macromolecules (nucleic acids and proteins) indicated that limonene damaged the cell membrane and increased membrane permeability. Besides, the reduction of membrane potential (MP) further confirmed that the damage to membrane and the reduction of respiratory metabolic activity. Furthermore, the respiratory depression test confirmed that the pathway of limonene affecting the respiratory metabolism of S. aureus is TCA pathway, followed by EMP. In addition, the disorder of succinate dehydrogenase (SDH), malate dehydrogenase (MDH), pyruvate kinase (PK), ATPase (T-ATPase, Na + -K + -ATPase, Ca 2+ -Mg 2+ -ATPase), and ATP concentration demonstrated that limonene could result in a slowdown of metabolism and metabolic dysfunction, thus inhibiting ATP synthesis. | INTRODUCTIONFood-borne pathogens are the leading cause of food-borne illness and one of the common pathogens causing food safety risks, causing great harm to human health (Qi et al., 2020;Tomao, Pirolo, Agnoletti, Pantosti, & Visca, 2020). Staphylococcus aureus (S. aureus), a Gram-positive bacterium with a arranged in spherical clusters shape under the microscope (Muniz et al., ), widely distributed in meat products (Rb et al., 2020), frozen food, and seafood, (Saklani, Lekshmi, Nayak, & Kumar, 2020). Staphylococcal enterotoxins are the causative toxins produced by S. aureus that can cause cutaneous lesions, pneumonia,

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Section: T-atpase Activitiesmentioning

confidence: 99%

Antimicrobial activity and mechanism of limonene against Staphylococcus aureus

Han

Chen

Sun

2021

Journal of Food Safety

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“…As depicted in Figure 4 B, the Na + /K + -ATPase activity of the treatment group was significantly improved. This outcome is probably due to the increase in membrane permeability and proton conversion caused by the disruption of the cell membrane structure [ 30 ].…”

Section: Resultsmentioning

confidence: 99%

Untargeted Metabolomics on Skin Mucus Extract of Channa argus against Staphylococcus aureus: Antimicrobial Activity and Mechanism

Leng

Shi

et al. 2021

Foods

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Microbial contamination is one of the most common food safety issues that lead to food spoilage and foodborne illness, which readily affects the health of the masses as well as gives rise to huge economic losses. In this study, Channa argus was used as a source of antimicrobial agent that was then analyzed by untargeted metabolomics for its antibacterial mechanism against Staphylococcus aureus. The results indicated that the skin mucus extract of C. argus had great inhibitory action on the growth of S. aureus, and the morphology of S. aureus cells treated with the skin mucus extract exhibited severe morphological damage under scanning electron microscopy. In addition, metabolomics analysis revealed that skin mucus extract stress inhibited the primary metabolic pathways of S. aureus by inducing the tricarboxylic acid cycle and amino acid biosynthesis, which further affected the normal physiological functions of biofilms. In conclusion, the antimicrobial effect of the skin mucus extract is achieved by disrupting cell membrane functions to induce an intracellular metabolic imbalance. Hence, these results conduce to amass novel insights into the antimicrobial mechanism of the skin mucus extract of C. argus against S. aureus.

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“…The metabolites were extracted according to Yuan et al [51]. Untargeted UPLC − QTOF-MS/MS was conducted according to Shu et al [52] by LipidALL Technologies Co., Ltd (Changzhou, China). Four biological replicates were performed independently.…”

Section: Metabonomic Analysismentioning

confidence: 99%

“…Data processing was performed according to Shu et al [52]. The unknown differential metabolites were qualitatively identified by the comparison with standards of HMDB, METLIN, and other database, and p < 0.05 in the one-way analysis of variance (ANOVA) and a fold change > 1.5 were considered to be the significant difference.…”

Section: Metabonomic Analysismentioning

confidence: 99%

Effect of overexpression of SNF1 on the transcriptional and metabolic landscape of baker’s yeast under freezing stress

Lü

Lin

et al. 2021

Microb Cell Fact

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Background Freezing stress is the key factor that affecting the cell activity and fermentation performance of baker’s yeast in frozen dough production. Generally, cells protect themselves from injury and maintain metabolism by regulating gene expression and modulating metabolic patterns in stresses. The Snf1 protein kinase is an important regulator of yeast in response to stresses. In this study, we aim to study the role of the catalytic subunit of Snf1 protein kinase in the cell tolerance and dough leavening ability of baker’s yeast during freezing. Furthermore, the effects of SNF1 overexpression on the global gene expression and metabolite profile of baker’s yeast before and after freezing were analysed using RNA-sequencing and untargeted UPLC − QTOF-MS/MS, respectively. Results The results suggest that overexpression of SNF1 was effective in enhancing the cell tolerance and fermentation capacity of baker’s yeast in freezing, which may be related to the upregulated proteasome, altered metabolism of carbon sources and protectant molecules, and changed cell membrane components. SNF1 overexpression altered the level of leucin, proline, serine, isoleucine, arginine, homocitrulline, glycerol, palmitic acid, lysophosphatidylcholine (LysoPC), and lysophosphatidylethanolamine (LysoPE) before freezing, conferring cells resistance in freezing. After freezing, relative high level of proline, lysine, and glycerol maintained by SNF1 overexpression with increased content of LysoPC and LysoPE. Conclusions This study will increase the knowledge of the cellular response of baker’s yeast cells to freezing and provide new opportunities for the breeding of low-temperature resistant strains.

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Metabolomics study on revealing the inhibition and metabolic dysregulation in Pseudomonas fluorescens induced by 3-carene

Cited by 39 publications

References 42 publications

Antimicrobial activity and mechanism of limonene against Staphylococcus aureus

Antimicrobial activity and mechanism of limonene against Staphylococcus aureus

Untargeted Metabolomics on Skin Mucus Extract of Channa argus against Staphylococcus aureus: Antimicrobial Activity and Mechanism

Effect of overexpression of SNF1 on the transcriptional and metabolic landscape of baker’s yeast under freezing stress

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