Metabonomic analysis of hepatitis B virus-induced liver failure: identification of potential diagnostic biomarkers by fuzzy support vector machine

Abstract

Hepatitis B virus (HBV)-induced liver failure is an emergent liver disease leading to high mortality. The severity of liver failure may be reflected by the profile of some metabolites. This study assessed the potential of using metabolites as biomarkers for liver failure by identifying metabolites with good discriminative performance for its phenotype. The serum samples from 24 HBV-induced liver failure patients and 23 healthy volunteers were collected and analyzed by gas chromatography-mass spectrometry (GC-MS) to generate metabolite profiles. The 24 patients were further grouped into two classes according to the severity of liver failure. Twenty-five commensal peaks in all metabolite profiles were extracted, and the relative area values of these peaks were used as features for each sample. Three algorithms, F-test, k-nearest neighbor (KNN) and fuzzy support vector machine (FSVM) combined with exhaustive search (ES), were employed to identify a subset of metabolites (biomarkers) that best predict liver failure. Based on the achieved experimental dataset, 93.62% predictive accuracy by 6 features was selected with FSVM-ES and three key metabolites, glyceric acid, cis-aconitic acid and citric acid, are identified as potential diagnostic biomarkers.

Response to weaning and dietary l-glutamine supplementation: metabolomic analysis in piglets by gas chromatography/mass spectrometry

“…Metabolomics is a useful system approach in the understanding of global changes of metabolites in animals in response to alterations in nutrition, genetics, environment, and gut microbiota (Nicholson et al, 2004;Mao et al, 2008). However, it has not been extensively used in the field of swine study.…”

“…Metabolomics, an important branch of systems biology, is defined as the quantitative measurement of small molecular metabolites in a biological sample (Mao et al, 2008;Nicholson and Lindon, 2008). It has offered novel insights into dietary effects by measuring and mathematically modeling changes in metabolic products (He et al, 2009).…”

Serum metabolomics analysis of patients with chikungunya and dengue mono/co-infections reveals distinct metabolite signatures in the three disease conditions

“…Among the various tools used for metabolomics, nuclear magnetic resonance (NMR) spectroscopy has emerged as an useful and powerful method due to its noninvasiveness, rapidity, reproducibility, need for small sample volumes, and high accuracy in metabolic profiling2324. NMR based metabolomics approach has been used to understand the metabolic dysregulation in humans upon viral infections like hepatitis252627 and HIV2829.…”

Development and validation of a liquid chromatography–mass spectrometry metabonomic platform in human plasma of liver failure caused by hepatitis B virus

“…So plasma was believed to provide a rich pool of disease-associated metabolites. Metabonomics, the unbiased identification and quantification of all the metabolites present in a specific biological sample (from an organism) [27], is very important in liver disease research [28,29].…”