Metabonomics Application on Screening Serum Biomarkers of Golden Hamsters with Nonalcoholic Steatohepatitis Induced by High-Fat Diet

Abstract

Background: Nonalcoholic steatohepatitis (NASH) is a common liver injury which will develop into advanced fibrosis and cirrhosis. This study was designed to identify the different serum metabolites of NASH hamsters and predict the diagnosis biomarkers for NASH. background: Nonalcoholic steatohepatitis (NASH) is the second leading cause of liver transplantation for hepatocellular carcinoma. In this study, metabonomics was used to identify the serum differential metabolites to study the relationship between metabolites and pathogenesis of NASH. Material and methods: Golden hamsters were randomly divided into a control group that received a normal diet and a NASH group that received a high-fat diet (HFD). After 12 weeks of feeding, the body and liver weight of the hamsters were monitored. Serum biochemical parameters and liver histopathological changes were analyzed. Moreover, an untargeted metabolomics analysis based on a GC-TOF/MS system was performed to identify the serum differential metabolites between the NASH and control groups. Results: The liver weight was increased in the NASH group, accompanied by significantly higher levels of serum TC, TG, ALT, AST, LDL-C, and lower HDL-C. HE, Masson, and oil red O staining showed the hepatocyte structure destroyed, lipid droplets accumulated, and fibers proliferated in the NASH group. Furthermore, 63 differential metabolites were identified by metabolomic analysis. Lipids and fatty acids were significantly up-regulated in the NASH group. The top 9 differential metabolites included cholesterol, methyl phosphate, taurine, alpha-tocopherol, aspartic acid, etc. Metabolites were mainly involved in amino acid metabolism (glycine, cysteine, taurine), spermine, fatty acid biosynthesis, urea cycle, bile acid metabolism pathways, etc. Conclusion: Metabonomics analysis identified 63 differential metabolites in the serum of NASH hamsters; among them, lipids and fatty acids had a key role and may be used as biomarkers for the early diagnosis of NASH. other: None.

PPARG and the PTEN-PI3K/AKT Signaling Axis May Cofunction in Promoting Chemosensitivity in Hypopharyngeal Squamous Cell Carcinoma

“…Fructus Choerospondiatis (FC) components were found to regulate the PPAR signaling pathway, indicating a potential relationship between PPARG and AKT1 in the treatment of coronary heart disease [8]. Additionally, studies involving interventions such as RES, Zhenwu decoction, Chinese angelica (CHA), and Fructus aurantii (FRA) all highlight PPARG and AKT1 as common targets, emphasizing their potential therapeutic significance in various conditions including diabetic kidney disease and colorectal cancer [19][20][21].…”