Metabonomics study on the hepatoprotective effect mechanism of polysaccharides from different processed products of Angelica sinensis on layer chickens based on UPLC–Q/TOF–MS/MS, multivariate statistical analysis and conjoint analysis

Abstract

Chicken colibacillosis is one of the most severe diseases in the poultry industry.Ceftiofur sodium (CS) is often used to treat it in clinical practice and lipopolysaccharide (LPS) accumulates in the chicken's body. Previous experimental studies found that CS combined with LPS could induce liver injury in layer chickens, and polysaccharides from charred Angelica sinensis(CASP) had a better hepatoprotective effect than polysaccharides from unprocessed Angelica sinensis(UASP). However, the intervention mechanism was unclear. Thus, UPLC-Q/TOF-MS/MS-based metabonomics and transcriptomics were used in this study to clarify the hepatoprotective effect mechanism of CASP and UASP in layer chickens. Transcriptomics and enzyme-linked immunosorbent assay were used for biological verification of some critical mutual metabolic pathways screened with metabonomics. The comprehensive analysis results showed that in a layer chicken liver injury model built with LPS and CS, 12 critical metabolic pathways were disturbed, involving 10 important differential metabolites. The hepatoprotective effect mechanism of CASP is related to the arachidonic acid metabolism and mTOR signaling pathways, involving nine important differential metabolites. In contrast, the hepatoprotective effect mechanism of UASP is related to the arachidonic acid metabolism pathway, involving six important differential metabolites.layer chickens, liver injury, LPS, metabonomics, polysaccharides from charred Angelica sinensis (CASP), polysaccharides from unprocessed Angelica sinensis(UASP)Abbreviations: ALT, alanine aminotransferase; AS, Angelica sinensis; AST, aspartate aminotransferase; CASP, polysaccharides from charred Angelica sinensis; CC, chicken colibacillosis; CS, ceftiofur sodium; FC, fold change; LPS, lipopolysaccharide; PLS-DA, partial least squares-discriminant analysis; UASP, polysaccharides from unprocessed AS; UPLC-Q/TOF-MS/MS, ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry/mass spectrometry; VIP, variable importance in projection; VN, Venn.

Study on the Hepatoprotective Effect Mechanism of Polysaccharides from Charred Angelica sinensis on the Layer Chickens Based on the Detection of the Intestinal Floras and Short-Chain Fatty Acids of Cecal Contents and Association Analysis

“…According to the analysis result, Faecalibacterium may be related to the enhancement of liver metabolism and growth promotion [ 35 ]. The KEGG function prediction results showed that the liver injury was mainly enriched in metabolic diseases, cell growth, and death, and the intervention effect on the liver injury of CASP was mainly enriched in amino acid metabolism, cell growth, and death, and the immune system, which was consistent with the previous conclusions [ 2 ].…”

“…It is confirmed that the liver protective effect of polysaccharides from charred Angelica sinensis (CASP) is accurate, and its intervention mechanism is related to arachidonic acid metabolic and mTOR pathway [ 2 ], but it is not clear whether it is related to the changes of intestinal floras and the short-chain fatty acids (SCFAs) content in the cecum.…”

Screening of polysaccharides from the differently processed products of Angelica sinensis with the best liver protection effect on chicken and the intervention mechanism study based on tandem mass tag proteomics and multiple reaction monitoring approach

“…ASP exhibits various biological activities such as liver protection (Wu, 2022), antioxidant properties (Ai et al, 2013), antitumor effects (Wang et al, 2018), and immune regulation (Meng, Wang, et al, 2022). Extensive literature reports and experimental evidence support the liver protection-related basic research on ASP, demonstrating its reparative effects on animal liver injury (Wang et al, 2020).…”