Metagenomic Analysis Exploring Taxonomic and Functional Diversity of Soil Microbial Communities in Sugarcane Fields Applied with Organic Fertilizer

Li, Ruoyu; Pang, Ziqin; Zhou, Yongmei; Fallah, Nyumah; Hu, Chaohua; Lin, Wenxiong; Yuan, Zhaonian

doi:10.1155/2020/9381506

Cited by 20 publications

(12 citation statements)

References 56 publications

(51 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, fertilizer concentrations significantly affect the germination percentage and need to be adjusted to optimum levels. The percentage of germinated seeds is in accordance with results described by other authors, who mention that organic fertilizers are nutritional supplements that improve the yield and growth of agricultural crops ( Gómez-Álvarez et al, 2008 ; Li et al, 2020 ). The reason for this is the increase in the availability of nutrients that are not easily assimilated by the plant under natural conditions and thus improve soil degradation.…”

Section: Discussionsupporting

confidence: 90%

Valorization as a biofertilizer of an agricultural residue leachate: Metagenomic characterization and growth promotion test by PGPB in the forage plant Medicago sativa (alfalfa)

Mora

Pastrana²,

Lobo³

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

The abuse of chemical fertilizers in intensive agriculture has turned out in the contamination of ground and the soil on which they are applied. Likewise, the generation, storage, and destruction of plant residues from the agri-food industry poses a threat to the environment and human health. The current situation of growing demand for food implies the urgent need to find sustainable alternatives to chemical fertilizers and the management of agricultural waste. Valorization of this plant residue to produce natural biofertilizers using microbiological treatments is presented as a sustainable alternative. The microbial activity allows the transformation into simple molecules that are easily absorbed by plants, as well as the stimulation of plant growth. This double direct and indirect action induced significant increases against the variables of germination, viability, and biomass (dry weight). To guarantee biosafety, it is necessary to use new bio-technological tools, such as metagenomics, which allow the taxonomic analysis of microbial communities, detecting the absence of pathogens. In the present paper, a physicochemical and metagenomic characterization of a fertilizer obtained from agricultural plant waste valorization is carried out. Likewise, fertigation treatments were tested to which the Plant Growth Promoting Bacteria (PGPB) Pseudomonas agronomica and Bacillus pretiosus were added, both independently and in consortium. Metagenomic analysis has identified taxa belonging to the kingdoms Bacteria and Archaea; 10 phyla, 25 families, 32 genera and 34 species, none of them previously described as pathogenic. A 1/512 dilution of the fertilizer increased the germination rate of Medicago sativa (alfalfa) by 16% at 144 h, compared to the treatment without fertilizer. Both the fertilizer and the addition of PGPB in a double direct and indirect action induced significant increases against the variables of germination, viability, and biomass (dry weight). Therefore, the use of an agricultural residue is proposed, which after the addition of two new species is transformed into a biofertilizer that significantly induces plant growth in Mendicago sativa plants.

show abstract

Section: Discussionsupporting

confidence: 90%

Valorization as a biofertilizer of an agricultural residue leachate: Metagenomic characterization and growth promotion test by PGPB in the forage plant Medicago sativa (alfalfa)

Mora

Pastrana²,

Lobo³

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…In contrast, 27 substances were increased by OF but not CF, including arabitol, threitol, inosine, glycerol, oleic acid, palmitic acid, dodecanol, thymine, 2-hydroxyvaleric acid, xylose, glucose-1-phosphate, glutaric acid, N-acetyl-D-hexosamine, deoxycholic acid, zymosterol, threonic acid, guanidinosuccinate, 2' -deoxyguanosine, citraconic acid, vanillic acid, glucosamine, phthalic acid, phytanic acid, inulotriose, inositol-4-monophosphate, malonic acid, and adipic acid were significantly increased (Table 7). In agreement with the result of this study, the application of bioorganic fertilizer caused the increase in the relative content of glucoheptulose, palmitic acid, phytanic acid, and deoxycholic acid [56,57]. Among the metabolites, inosine is reported to be essential for the growth of plants, which is involved in phosphatidylinositol signal transduction, auxin storage and transportation, phytic acid biosynthesis, cell wall biosynthesis and plant response to stress [58], and it can protect the cell membrane system by reducing the damage of membrane lipid peroxidation and enhancing the activity of antioxidant enzymes [34,54].…”

Section: The Effect Of Compound/bio-organic Fertilizer On Rhizosphere Soil Metabolomicssupporting

confidence: 92%

Effect of Two Kinds of Fertilizers on Growth and Rhizosphere Soil Properties of Bayberry with Decline Disease

Ren

Wang

et al. 2021

Plants

View full text Add to dashboard Cite

Decline disease causes severe damage to bayberry. However, the cause of this disease remains unclear. Interestingly, our previous studies found that the disease severity is related with the level of soil fertilizer. This study aims to explore the effect and mechanism of compound fertilizer (CF) and bio-organic fertilizer (OF) in this disease by investigating the vegetative growth, fruit characters, soil property, rhizosphere microflora and metabolites. Results indicated that compared with the disease control, CF and OF exhibited differential effect in plant healthy and soil quality, together with the increase in relative abundance of Burkholderia and Mortierella, and the reduction in that of Rhizomicrobium and Acidibacter, Trichoderma, and Cladophialophora reduced. The relative abundance of Geminibasidium were increased by CF (251.79%) but reduced by OF (13.99%). In general, the composition of bacterial and fungal communities in rhizosphere soil was affected significantly at genus level by exchangeable calcium, available phosphorus, and exchangeable magnesium, while the former two variables had a greater influence in bacterial communities than fungal communities. Analysis of GC-MS metabonomics indicated that compared to the disease control, CF and OF significantly changed the contents of 31 and 45 metabolites, respectively, while both fertilizers changed C5-branched dibasic acid, galactose, and pyrimidine metabolic pathway. Furthermore, a significant correlation was observed at the phylum, order and genus levels between microbial groups and secondary metabolites of bayberry rhizosphere soil. In summary, the results provide a new way for rejuvenation of this diseased bayberry trees.

show abstract

“…Liu et al ( 2021 ) found that intercropping alfalfa with oat provided rich nutrients for soil microorganisms and ultimately affected the change of soil microbial dominant population and quantity due to the litter and root exudates. A higher soil microbial diversity index is more conducive to improving the stability and resistance of soil ecosystems, ensuring the normal operation of soil ecosystem function (Li et al, 2020b ). The α-diversity indices, including Chao1 and ACE, reflected the richness of the soil bacterial community, and the Shannon index reflected the evenness of soil bacterial community, which was positively correlated with the diversity of the soil bacterial community (Fan et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Intercropping Pinto Peanut in Litchi Orchard Effectively Improved Soil Available Potassium Content, Optimized Soil Bacterial Community Structure, and Advanced Bacterial Community Diversity

Zhao

Yan

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

Intercropping is widely used in agricultural production due to its capability of raising land productivity and providing an opportunity to achieve sustainable intensification of agriculture. In this study, soil samples from 10 to 20 cm depth of intercropping Pinto peanut in litchi orchard and litchi monoculture mode were established to determine soil attributes, enzyme activities, as well as the effect on soil bacterial diversity. On this basis, 16S rRNA V4-V5 region of soil bacterial communities in litchi/Pinto peanut intercropping (LP) mode and litchi monoculture mode (CK) was detected by the Illumina MiSeq sequencing platform. The results showed that the content of available potassium (AK) in LP was significantly higher than that in CK by 138.9%, and the content of available nitrogen (AN) in LP was significantly lower than that in CK by 19.6%. The soil enzyme activities were higher in LP as a whole, especially sucrase (SC) and acid protease (PT) were significantly higher by 154.4 and 76.5%, respectively. The absolute abundance and alpha diversity of soil microbiota were significantly higher in the intercropping group. Most importantly, endemic species with a significant difference in LP was higher by ~60 times compared to CK treatment. In the aspect of soil bacterial community structure, the dominant phyla of the two groups were Acidobacteria, Proteobacteria, Chloroflexi, and Actinobacteria. At the genus level, the absolute abundance of Flavobacterium and Nitrososphaera was significantly higher by 79.20 and 72.93%, respectively, while that of Candidatus_Koribacter was significantly lower with an amplitude of 62.24% in LP than in CK. Furthermore, the redundancy analysis (RDA) suggested that AK, which was highly associated with the dominant genera and phyla, is the vitally dominating environmental factors in LP groups, while in CK groups, it is AN and pH. In addition, PICRUSt2 analysis indicated that intercropping improved the metabolic activity of bacteria which can be correlated to the resistance of litchi root systems to soil-borne diseases. Overall, this study is expected to provide a theoretical basis and technical support for the healthy intercropping cultivation of litchi.

show abstract

Metagenomic Analysis Exploring Taxonomic and Functional Diversity of Soil Microbial Communities in Sugarcane Fields Applied with Organic Fertilizer

Cited by 20 publications

References 56 publications

Valorization as a biofertilizer of an agricultural residue leachate: Metagenomic characterization and growth promotion test by PGPB in the forage plant Medicago sativa (alfalfa)

Valorization as a biofertilizer of an agricultural residue leachate: Metagenomic characterization and growth promotion test by PGPB in the forage plant Medicago sativa (alfalfa)

Effect of Two Kinds of Fertilizers on Growth and Rhizosphere Soil Properties of Bayberry with Decline Disease

Intercropping Pinto Peanut in Litchi Orchard Effectively Improved Soil Available Potassium Content, Optimized Soil Bacterial Community Structure, and Advanced Bacterial Community Diversity

Contact Info

Product

Resources

About