Metagenomic analysis of individually analyzed ticks from Eastern Europe demonstrates regional and sex-dependent differences in the microbiota of Ixodes ricinus

Batool, Maliha; Blazier, John C.; Rogovska, Yuliya V.; Wang, Jiangli; Liu, Shilin; Nebogatkin, Igor; Rogovskyy, Artem S.

doi:10.1016/j.ttbdis.2021.101768

Cited by 16 publications

(28 citation statements)

References 53 publications

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“…41 Moreover, Batool and colleagues found that in Ukraine, that is a neighboring country to Hungary with an area of around 6.5 times as big, the alpha diversity analyses demonstrated differences in tick microbiota patterns of various administrative regions. 42 As of the dissimilarity of the samples’ core bacteriome genus profiles (beta diversity), inter-regional comparison of developmental stages (females or nymphs) produced significant differences, while developmental stage-wise testing of climatic condition associated localization did not. Similarly, Batool and colleagues described that tick sex comparisons resulted in significant differences on various beta-diversity tests regardless of the area of origin.…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, Batool and colleagues described that tick sex comparisons resulted in significant differences on various beta-diversity tests regardless of the area of origin. 42 While these results are interesting to compare, different study models and testing methods should be taken into account.…”

Section: Discussionmentioning

confidence: 99%

“…Certain studies presented relatively low rickettsial genome fragment counts. 42 The reason for these alterations is that the appearance rates of Rickettsia spp. vary between the geographical populations of ticks.…”

Section: Discussionmentioning

confidence: 99%

“…60,61 Even though, certain bacteria, such as cutibacteria and staphylococci may be considered as bacterial contaminants from the sample processing steps, 15 according to a recent study, the effects of a possible, minor level contamination are not noticeable in the overall relative bacterial abundance. 42 Nevertheless, it is possible, that some environmental genera were only present at the cuticle of the collected ticks regardless of the repeated laboratory washing steps with 99.8% alcohol.…”

Section: Discussionmentioning

confidence: 99%

“…also appears to be dependent on the region of tick collection 68 and, as described earlier, staphylococcal hits may also derive from contamination. 42…”

Section: Discussionmentioning

confidence: 99%

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Ixodes ricinustick bacteriome alterations based on a climatically representative survey in Hungary

Tóth

Farkas

Papp

et al. 2022

Preprint

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Background: The microbial communities of disease vectors may represent a key feature in several biological functions, and thus deserve special attention in light of climate change and the consequent need for the development of novel control strategies. Nevertheless, vector-borne microbial networks are still poorly understood. Assessing the microbial interactions and climatic dependencies of vectors may contribute to better estimating pathogen transmission characteristics, public health risks and the urge for control steps. Results: After the collection of Ixodes ricinus ticks from a climatically representative set of sampling points in Hungary and the next generation sequencing based acquisition of metagenomic shotgun sequencing datasets. Furthermore, reports of the core bacteriome constituting of higher agglomerated genus-level relative abundance read counts than 1% in at least one of the samples, along with and β-diversity and the statistically significant differences and correlations of the genus-level bacterial relative abundances were presented in various developmental stage tick groups. Besides these, the identified pathogenic bacteria were also listed. Genera constituting the core bacteriome (Arsenophonus, Bacillus, Candidatus Midichloria, Curtobacterium, Cutibacterium, Mycobacteroides, Pseudomonas, Rhodococcus, Rickettsia, Sphingomonas, Staphylococcus, Stenotrophomonas and Wolbachia) showed several significant relative abundance differences and correlations among developmental stages and at sampling points with different climatic conditions. Reads of pathogenic bacteria from the genera of Anaplasma, Borrelia, Borreliella, Ehrlichia, Rickettsia were associated with the developmental stage and the sampling geolocation of the host ticks. Conclusions: The composition and correlations of vector-borne microbiota members showed significant alterations at sampling points with different temperature and precipitation histories and at the various developmental stages of the tick hosts. Our findings not only pave the way towards the understanding of tick-borne bacterial networks and interdependencies, but also raise light to the high potential for the presence of a possible biological tick control species, the tick parasitoid, Ixodiphagus hookeri based on related bacteriome patterns. The results of conscious tick microbiome assessment studies may contribute to the precision tick control strategies of the future.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…also appears to be dependent on the region of tick collection 68 and, as described earlier, staphylococcal hits may also derive from contamination. 42…”

Section: Discussionmentioning

confidence: 99%

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