Metagenomic analysis of microbial community of a parasitoid wasp Megaphragma amalphitanum

Nedoluzhko, Artem; Sharko, Fedor; Tsygankova, Svetlana; Boulygina, Eugenia S.; Sokolov, Alexey; Расторгуев, С. М.; Kadnikov, Vitaly V.; Mardanov, Andrey V.; Ravin, Nikolai V.; Mazur, Alexander; Polilov, Alexey A.; Gruzdeva, Natalia; Prokhortchouk, Egor; Skryabin, K. G.

doi:10.1016/j.gdata.2016.12.007

Cited by 10 publications

(10 citation statements)

References 11 publications

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“…Only a handful of papers reported the characterization of parasitoids' microbiome by molecular methods 43–46 . In those, a diverse community of bacteria was found, most of which was attributed to environmental microbes, associated with gut activity or transient.…”

Section: Discussionmentioning

confidence: 99%

Wolbachia influence on the fitness of Anagyrus vladimiri (Hymenoptera: Encyrtidae), a bio‐control agent of mealybugs

Izraeli

Lalzar

Netanel

et al. 2020

Pest Management Science

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BACKGROUND Like numerous other animals, biocontrol agents (BCAs) of arthropod pests carry various microorganisms that may have diverse effects on the biology of their eukaryote hosts. We postulated that it is possible to improve the efficacy of BCAs by manipulating the composition of their associated microbiota. The parasitoid wasp Anagyrus vladimiri (Hymenoptera: Encyrtidae) from a mass‐rearing facility was chosen for testing this hypothesis. RESULTS High‐throughput sequencing analysis indicated that fungal abundance in A. vladimiri was low and variable, whereas the bacterial community was dominated by the endosymbiont Wolbachia. Wolbachia was fixed in the mass‐rearing population, whereas in field‐collected A. vladimiri Wolbachia's prevalence was only approximately 20%. Identification of Wolbachia strains from the two populations by Multi Locus Sequence Typing, revealed two closely related but unique strains. A series of bioassays with the mass‐rearing Wolbachia‐fixed (W+) and a derived antibiotic‐treated Wolbachia‐free (W–) lines revealed that: (i) Wolbachia does not induce reproductive manipulations; (ii) W– females have higher fecundity when reared individually, but not when reared with conspecifics; (iii) W+ females outcompete W– when they share hosts for oviposition; (iv) longevity and developmental time were similar in both lines. CONCLUSIONS The findings suggest that W+ A. vladimiri have no clear fitness benefit under mass‐rearing conditions and may be disadvantageous under lab‐controlled conditions. In a broader view, the results suggest that augmentative biological control can benefit from manipulation of the microbiome of natural enemies.

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Section: Discussionmentioning

confidence: 99%

Wolbachia influence on the fitness of Anagyrus vladimiri (Hymenoptera: Encyrtidae), a bio‐control agent of mealybugs

Izraeli

Lalzar

Netanel

et al. 2020

Pest Management Science

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“…Little attention has been given to the general bacterial community of parasitoids. Next-generation sequencing and diagnostic PCR have been used to characterize the bacterial community present in Nasonia species (10), Asobara tabida (119), Megaphragma amalphitanum (69), the Eretmocerus species Eretmocerus mundus and Eretmocerus eremicus, and Encarsia formosa (22). In Nasonia species, species of the bacterial genera Providencia and Acinetobacter were most frequently recorded (10).…”

Section: Symbiont Diversity and Transmission In Parasitoids Diversitymentioning

confidence: 99%

Microbial Symbionts of Parasitoids

Dicke

Cusumano

Poelman

2020

Annu. Rev. Entomol.

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Parasitoids depend on other insects for the development of their offspring. Their eggs are laid in or on a host insect that is consumed during juvenile development. Parasitoids harbor a diversity of microbial symbionts including viruses, bacteria, and fungi. In contrast to symbionts of herbivorous and hematophagous insects, parasitoid symbionts do not provide nutrients. Instead, they are involved in parasitoid reproduction, suppression of host immune responses, and manipulation of the behavior of herbivorous hosts. Moreover, recent research has shown that parasitoid symbionts such as polydnaviruses may also influence plant-mediated interactions among members of plant-associated communities at different trophic levels, such as herbivores, parasitoids, and hyperparasitoids. This implies that these symbionts have a much more extended phenotype than previously thought. This review focuses on the effects of parasitoid symbionts on direct and indirect species interactions and the consequences for community ecology.

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“…No traces of Wolbachia infection or other representatives of the Rickettsiaceae family were found in M . amalphitanum individuals [62], while the sequenced T . pretiosum carries the Wolbachia symbiont [63]; the sequenced Nasonia strain was maintained on antibiotics to cure it of infection.…”

Section: Resultsmentioning

confidence: 99%

A partial genome assembly of the miniature parasitoid wasp, Megaphragma amalphitanum

Sharko¹,

Nedoluzhko²,

Lê³

et al. 2019

PLoS ONE

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Body size reduction, also known as miniaturization, is an important evolutionary process that affects a number of physiological and phenotypic traits and helps animals conquer new ecological niches. However, this process is poorly understood at the molecular level. Here, we report genomic and transcriptomic features of arguably the smallest known insect–the parasitoid wasp, Megaphragma amalphitanum (Hymenoptera: Trichogrammatidae). In contrast to expectations, we find that the genome and transcriptome sizes of this parasitoid wasp are comparable to other members of the Chalcidoidea superfamily. Moreover, compared to other chalcid wasps the gene content of M. amalphitanum is remarkably conserved. Intriguingly, we observed significant changes in M. amalphitanum transposable element dynamics over time, in which an initial burst was followed by suppression of activity, possibly due to a recent reinforcement of the genome defense machinery. Overall, while the M. amalphitanum genomic data reveal certain features that may be linked to the unusual biological properties of this organism, miniaturization is not associated with a large decrease in genome complexity.

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Metagenomic analysis of microbial community of a parasitoid wasp Megaphragma amalphitanum

Cited by 10 publications

References 11 publications

Wolbachia influence on the fitness of Anagyrus vladimiri (Hymenoptera: Encyrtidae), a bio‐control agent of mealybugs

Wolbachia influence on the fitness of Anagyrus vladimiri (Hymenoptera: Encyrtidae), a bio‐control agent of mealybugs

Microbial Symbionts of Parasitoids

A partial genome assembly of the miniature parasitoid wasp, Megaphragma amalphitanum

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