Metagenomic Next-Generation Sequencing Reveals the Profile of Viral Infections in Kidney Transplant Recipients During the COVID-19 Pandemic

Tian, Xinxin; Duan, Wenjing; Zhang, Xiulei; Wu, Xiaoqiang; Zhang, Chan; Wang, Zhiwei; Cao, Guanghui; Gu, Yue; Shao, Fengmin; Yan, Tianzhong

doi:10.3389/fpubh.2022.888064

Cited by 5 publications

(1 citation statement)

References 47 publications

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“…The species of voles and mice in which VPXV is found have minimal contact with humans, so there is believed to be a low chance for transmission [105]. VPXV has been incidentally found upon metagenomic next-generation sequencing to detect viral infections in kidney transplant recipients; however, the clinical significance is unclear [106]. Initial symptoms reported among mice infected with VPXV include skin lesions followed by decreased activity level, conjunctivitis, and facial edema.…”

Section: Clinical Manifestations and Transmissionmentioning

confidence: 99%

Ophthalmic Features and Implications of Poxviruses: Lessons from Clinical and Basic Research

et al. 2022

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Amidst the ongoing monkeypox outbreak, global awareness has been directed towards the prevention of viral transmission and case management, with the World Health Organization declaring the outbreak a public health emergency of international concern. Monkeypox virus is one of several species in the Orthopoxvirus genus, with other species of the genus including the variola, cowpox, mousepox, camelpox, raccoonpox, skunkpox, and volepox viruses. Although the nomenclature of these species is based on the animal host from which they were originally isolated, transmission from animals to humans has been reported with several species. The progression of disease, following an incubation period, typically consists of a prodromal phase with systemic flu-like symptoms. Various organ systems may be affected in addition to the formation of pathognomonic skin lesions. As monkeypox poses a continued public health concern, the ophthalmic sequelae of monkeypox virus, especially those leading to vision loss, warrant consideration as well. This review provides a comprehensive summary of the ophthalmic implications of poxviruses in clinical and laboratory settings reported in the literature, as well as areas of unmet need and future research.

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Section: Clinical Manifestations and Transmissionmentioning

confidence: 99%

Ophthalmic Features and Implications of Poxviruses: Lessons from Clinical and Basic Research

et al. 2022

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Urinary cytomegalovirus excretion: The unresolved issues

Puget,

Node,

Caël

et al. 2024

Annales Pharmaceutiques Françaises

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Comparison between metagenomic next-generation sequencing in bronchoalveolar lavage fluid and blood for the diagnostic performance of respiratory tract infection: a meta-analysis

Sun,

Liang,

Niu

2024

Front. Bacteriol.

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ObjectiveMetagenomic next-generation sequencing (mNGS) is emerging to be pivotal in infectious disease diagnosis, particularly in pulmonary infection. However, the utility of bronchoalveolar lavage fluid (BALF) versus blood mNGS remains controversial. Therefore, the current meta-analysis summarized the previous studies regarding BALF or blood mNGS in pulmonary infection, aiming to comprehensively compare the diagnostic efficiency between them.MethodsStudies reporting paired BALF and blood mNGS data for pulmonary infection diagnosis were searched in the PubMed, Web of Science, Embase, CNKI, and Wanfang from January 2016 to March 2024.ResultsEleven studies involving 346 assessed patients were eligible and analyzed. The pooled sensitivity, specificity, and area under the curve (AUC) of BALF mNGS were 0.94 [95% confidence interval (CI): 0.90–0.97], 0.27 (95% CI: 0.19–0.36), and 0.86 [standard error (SE): 0.06], respectively. Meanwhile, the pooled sensitivity, specificity, and AUC of blood mNGS were 0.64 (95% CI: 0.56–0.72), 0.69 (95% CI: 0.62–0.76), and 0.81 (SE: 0.05), respectively. By subgroup analyses, the AUCs of BALF mNGS and blood mNGS for viral detection were 0.70 (SE: 0.08) and 0.71 (SE: 0.08), respectively, while the AUCs of BALF mNGS and blood mNGS for nonviral (bacterial or fungal) detection were 0.83 (SE: 0.06) and 0.73 (SE: 0.08), respectively. Moreover, no threshold effect or publication bias existed, and sensitivity analysis revealed that the findings were generally robust.ConclusionBALF mNGS surpasses blood mNGS for total pathogen detection in pulmonary infection patients, while they share a similar efficiency for viral detection.Systematic review registrationhttps://www.crd.york.ac.uk/PROSPERO/, identifier CRD42024562740.

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Metagenomic Next-Generation Sequencing Reveals the Profile of Viral Infections in Kidney Transplant Recipients During the COVID-19 Pandemic

Cited by 5 publications

References 47 publications

Ophthalmic Features and Implications of Poxviruses: Lessons from Clinical and Basic Research

Ophthalmic Features and Implications of Poxviruses: Lessons from Clinical and Basic Research

Urinary cytomegalovirus excretion: The unresolved issues

Comparison between metagenomic next-generation sequencing in bronchoalveolar lavage fluid and blood for the diagnostic performance of respiratory tract infection: a meta-analysis

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