Metagenomic profiles of soil microbiota under two different cropping systems detected by STRs-based PCR

Abstract

In this research, soil microbial structures under a wheat triennial monoculture and horse beanwheat-horse bean succession were evidenced using a metagenomic approach. Polymorphism analysis of DNA extracted from soil samples collected at the end of the third year of the two crop successions, was performed by PCR, carried-out with six different primers designed on simple tandem repeats sequences. Readable profiles were obtained with M13 primer, from which no polymorphisms were detected, and with the primer (GACA) 4 , that gave distinctive patterns. Experimental findings suggest that metagenomic analysis performed by (GACA) 4 primer may be an easy and suitable method to discriminate microbial diversity of different crop successions. (GACA) 4 PCR-pattern indicate that soil microbiota changes are well correlated with crop succession.

Developing lactic acid bacteria starter cultures for wholemeal rye flour bread with improved functionality, nutritional value, taste, appearance and safety

“…Two oligonucleotides, B10 (5'-CTGCTGCTGGGAC-3') [29,30] and GACA (5'-GACAGA-CAGACAGACA-3') [31,32] microsatellite primers were used for isolates biotyping. Amplification reactions were performed in a 30 μl reaction volume containing: 3 μl of 10x ExTaq buffer, 250 μmol l -1 each of dATP, dCTP, dGTP and dTTP, 1 μmol l -1 primer, 100 ng genomic DNA and 2 U ExTaq DNA polymerase (TaKaRa Bio inc, Kusatsu, Japan).…”