Monomolecular films of an Oligo(phenylene)ethynylene (OPE) derivative [2isocyano-1,3-dimethylbenzene][4-(4′-amino-phenylethynyl)-phenylethynyl]-gold, 1, containing a gold atom in the molecule backbone have been prepared by the Langmuir-Blodgett (LB) method in order to study how the electrical properties can be modulated in monolayers of OPEs by the incorporation of a gold center in their structures. UV-vis reflection spectra of Langmuir monolayers of 1 at the air-water interface reveal strong aurophilic interactions between neighbouring molecules that increase upon compression. Monolayer Langmuir-Blodgett (LB) films were readily fabricated by the transfer of Langmuir films of 1 onto solid substrates. Quartz crystal microbalance (QCM) experiments conclusively demonstrate the formation of monolayer LB films with a high surface coverage. The morphology of these films was analysed by atomic force microscopy (AFM), revealing the formation of homogeneous layers with an optimum surface pressure of transference of 6 mN•m-1. Film homogeneity and integrity was confirmed by cyclic voltammetry, with efficient blocking of gold electrodes by these well-formed monolayers of 1. The electrical properties of LB films of 1 were investigated by scanning tunnelling microscopy (STM) using a 'tip-to-contact' method. Symmetrical, sigmoidal-shaped I-V curves were observed, with analysis of the pseudolinear (Ohmic) region giving a conductance value G = 3.9 10-5 G 0 , which is relatively high for an OPE derivative and may indicate a beneficial role of metal atom incorporation within the wire-like-system.