In order to investigate the anthropogenic and natural sources of metals in the Gulf of Prigi, Indonesia, it was analyzed their metal contents and Pb isotopes of surface seawater together with sediment, grouper fish, mosh, seaweed, soil, and sharp grass for protecting the environmental pollution of the study area. Samples were leached using a microwave digestion technique. Metal (Ca, Fe, Mn, Cu and Pb) concentrations and 206 Pb, 207 Pb and 208 Pb in the leached solutions were analyzed using inductively coupled plasma-mass spectrometry (ICP-MS). The level concentrations of Ca (491436 μg kg-1), Fe (12123 μg kg-1), Mn (37.2 μg kg-1), Cu (84.0 μg kg-1) and Pb (34.5 μg kg-1) in the surface seawater were low compared with those of background seawater levels issued by World Health Organization (WHO). The range of Pb isotope ratios (207 Pb / 206 Pb = 0.7966-0.9945 and 208 Pb / 206 Pb = 2.2600-2.8243) in surface seawater was higher in the plotted Pb growth curve suggesting unpolluted Pb reflected by natural sources such as mineral sources or lithogenic from marine sediment.