Metal-enhanced intrinsic fluorescence of nucleic acids using platinum nanostructured substrates

Akbay, Nuriye; Mahdavi, F.; Lakowicz, Joseph R.; Ray, Krishanu

doi:10.1016/j.cplett.2012.08.020

Cited by 17 publications

(12 citation statements)

References 26 publications

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“…The cyclodextrin spectrum is dominated by C–O, C–C–O, and C–O–C stretching vibration modes of the glucopyranose units in the range of 1200–900 cm –1 . 42 The light gray spectrum, corresponding to the antibiotic cast film, is characterized by the IR features typical of the macrocyclic lactone, and in particular by the C=O stretching mode of the cyclic ester absorption located at 1721 cm –1 , and of the oligosaccharide moieties, typically C–O stretching modes in the 1200–900 cm –1 interval. A cast film of an aqueous solution containing both cyclodextrin and antibiotic in the proper molar ratio (18 × 10 –8 and 6 × 10 –8 mol, respectively) was further obtained on the ATR plate (gray spectrum, Figure 3 A).…”

Section: Results and Discussionmentioning

confidence: 99%

“…41 Fourier-transform infrared (FT-IR) spectroscopy measurements were carried out to characterize the methyl-β-cyclodextrin@spiramycin I complex and individuate the chemical groups involved in the binding mechanism (Figure 3). Dropcasted films starting from an aqueous solution containing 18 × 10 −8 mol of methyl-β-cyclodextrin and 6 × 10 −8 mol of spiramycin I were obtained directly on the attenuated total reflectance (ATR) plate, and the spectra were recorded after water evaporation, black and light gray spectra in Figure 3A 42 The light gray spectrum, corresponding to the antibiotic cast film, is characterized by the IR features typical of the macrocyclic lactone, and in particular by the CO stretching mode of the cyclic ester absorption located at 1721 cm −1 , and of the oligosaccharide moieties, typically C−O stretching modes in the 1200−900 cm −1 interval. A cast film of an aqueous solution containing both cyclodextrin and antibiotic in the proper molar ratio (18 × 10 −8 and 6 × 10 −8 mol, respectively) was further obtained on the ATR plate (gray spectrum, Figure 3A).…”

Section: ■ Introductionmentioning

confidence: 99%

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Stimulatory Effects of Methyl-β-cyclodextrin on Spiramycin Production and Physical–Chemical Characterization of Nonhost@Guest Complexes

Calcagnile¹,

Bettini²,

Damiano³

et al. 2018

ACS Omega

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Spiramycin is a macrolide antibiotic and antiparasitic that is used to treat toxoplasmosis and various other infections of soft tissues. In the current study, we evaluated the effects of α-cyclodextrin, β-cyclodextrin, or methyl-β-cyclodextrin supplementation to a synthetic culture medium on biomass and spiramycin production by Streptomyces ambofaciens ATCC 23877. We found a high stimulatory effect on spiramycin production when the culture medium was supplemented with 0.5% (w/v) methyl-β-cyclodextrin, whereas α-cyclodextrin or β-cyclodextrin weakly enhanced antibiotic yields. As the stimulation of antibiotic production could be because of spiramycin complexation with cyclodextrins with effects on antibiotic stability and/or efflux, we analyzed the possible formation of complexes by physical–chemical methods. The results of Job plot experiment highlighted the formation of a nonhost@guest complex methyl-β-cyclodextrin@spiramycin I in the stoichiometric ratio of 3:1 while they excluded the formation of complex between spiramycin I and α- or β-cyclodextrin. Fourier-transform infrared spectroscopy measurements were then carried out to characterize the methyl-β-cyclodextrin@spiramycin I complex and individuate the chemical groups involved in the binding mechanism. These findings may help to improve the spiramycin fermentation process, providing at the same time a new device for better delivery of the antibiotic at the site of infection by methyl-β-cyclodextrin complexation, as it has been well-documented for other bioactive molecules.

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Section: Results and Discussionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Stimulatory Effects of Methyl-β-cyclodextrin on Spiramycin Production and Physical–Chemical Characterization of Nonhost@Guest Complexes

Calcagnile¹,

Bettini²,

Damiano³

et al. 2018

ACS Omega

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“…Our synthesized PtNPs do not exert fluorescence themselves (data not shown). However, nanoparticles produced by other scientists may have such capabilities 26 , 27 . Figure 2B shows spectra of PtNPs (primary concentration: 32 µg/mL) titrated with increasing amounts of ICR-191 (concentration range: 13–123 µM), while Fig.…”

Section: Resultsmentioning

confidence: 99%

Interactions of newly synthesized platinum nanoparticles with ICR-191 and their potential application

Borowik

Banasiuk

Derewońko

et al. 2019

Sci Rep

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One of the greatest challenges of modern medicine is to find cheaper and easier ways to produce transporters for biologically active substances, which will provide selective and efficient drug delivery to the target cells, while causing low toxicity towards healthy cells. Currently, metal-based nanoparticles are considered a successful and viable solution to this problem. In this work, we propose the use of novel synthesis method of platinum nanoparticles (PtNPs) connected with their precise biophysical characterization and assessment of their potential toxicity. To work as an efficient nanodelivery platform, nanoparticles should interact with the desired active compounds spontaneously and non-covalently. We investigated possible direct interactions of PtNPs with ICR-191, a model acridine mutagen with well-established biophysical properties and mutagenic activity, by Dynamic Light Scattering, fluorescence spectroscopy, and Isothermal Titration Calorimetry. Moreover, to determine the biological activity of ICR-191-PtNPs aggregates, we employed Ames mutagenicity test, eukaryotic cell line analysis and toxicity test against the model organism Caenorhabditis elegans. PtNPs’ interesting physicochemical properties associated to the lack of toxicity in a tested range of concentrations, as well as their ability to modulate ICR-191 biological activity, suggest that these particles successfully work as potential delivery platforms for different biologically active substances.

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“…Surface plasmons were also used for enhancing fluorescence at the DUV excitation. Al, In, and Pt have been used. The detection of nucleic acids and proteins were discussed in literature.…”

Section: Duv Plasmon‐enhanced Molecular Sensing and Imagingmentioning

confidence: 99%

Deep‐Ultraviolet Biomolecular Imaging and Analysis

Kumamoto

Taguchi

Kawata

2018

Advanced Optical Materials

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Deep‐ultraviolet light, 200–300 nm in wavelength, interacts with nucleic acids and proteins strongly compared to visible and infrared light. In this article, the interaction between deep‐ultraviolet photons and biomolecules is discussed. Especially, the absorption and autofluorescence of biomolecules by the deep‐ultraviolet excitation are examined. Applications of deep‐ultraviolet absorption and autofluorescence to label‐free biomolecular imaging and analysis of cells and tissues are shown. Resonant Raman scattering at nucleotide bases and aromatic amino acids as another important topic of deep‐ultraviolet photonics is reviewed in biomolecular imaging and analysis. The discussion extends to the recent progress in the development of deep‐ultraviolet microscopes as well as a variety of deep‐ultraviolet optical devices such as light sources, detectors, and objectives. The issue of photodamage due to deep‐ultraviolet irradiation on cells and tissues is also discussed. It has been recently suppressed with use of lanthanide ions. The experimental results of the photodamage suppression are shown. For surface‐enhanced resonant Raman scattering, autofluorescence enhancement, and tip‐enhanced resonant Raman scattering microscopy, plasmonic materials applicable in the deep‐ultraviolet range are discussed.

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Metal-enhanced intrinsic fluorescence of nucleic acids using platinum nanostructured substrates

Cited by 17 publications

References 26 publications

Stimulatory Effects of Methyl-β-cyclodextrin on Spiramycin Production and Physical–Chemical Characterization of Nonhost@Guest Complexes

Stimulatory Effects of Methyl-β-cyclodextrin on Spiramycin Production and Physical–Chemical Characterization of Nonhost@Guest Complexes

Interactions of newly synthesized platinum nanoparticles with ICR-191 and their potential application

Deep‐Ultraviolet Biomolecular Imaging and Analysis

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