Two-dimensional transition-metal dichalcogenides (TMDs) have attracted widespread interests owing to their attractive physical and chemical properties. In particular, they are especially attractive in the field of electrochemical biosensors. In this study, the properties of TMDs ((molybdenum sulfide (MoS 2 ), molybdenum diselenide (MoSe 2 ) and molybdenum telluride (MoTe 2 )) on the glucose sensing were explored and compared. Exfoliated MoS 2 , MoSe 2 and MoTe 2 nanosheets with basically the same size were fabricated through controlling the ultrasonic power. The structure and morphological characteristics of MoS 2 , MoSe 2 and MoTe 2 were examined through scanning electron microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy and powder X-ray diffractometer.Electrochemical properties were analyzed through cyclic voltammetry and differential pulse voltammetry. Results showed that all the three kinds of TMDs can enhance the electroconductibility of the electrodes. When incorporated into the construction of electrochemical biosensors for glucose detection, all of the three kinds of exfoliated TMDs modified electrodes possessed achieved analytical requirements of selectivity, wide linear ranges, as well as low limits of detection and quantification. In particular, MoS 2 -based biosensor generated a higher specificity and lower detection limit than that of MoSe 2 and MoTe 2 . This is imperative to showcase the real potential of TMDs in electrochemical biosensors.