Metal Ion Homeostasis Mediated by NRAMP Transporters in Plant Cells - Focused on Increased Resistance to Iron and Cadmium Ion

Sano, Takeshi; Yoshihara, Toshihiro; Handa, Koichi; Sato, Masa H.; Nagata, Toshiyuki; Hasezawa, Seiichiro

doi:10.5772/30905

Cited by 11 publications

(19 citation statements)

References 56 publications

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“…6). These results are in agreement with the study on BY-2 tobacco cells that indicated down-regulation of NtZIP1 by Fe excess (Sano et al , 2012), and with known regulation of AtVTL1 by Fe deficiency in Arabidopsis (Gollhofer et al , 2014). Thus enhanced expression of NtZIP1 , NtZIP2 , and NtMTP1A and decreased expression of NtVTL in the apical segments of roots grown at 4 µM Cd might suggest a low Zn and/or Fe status at the cellular level, although in these root fragments a decrease in the total metal concentration was noted only for Fe, and only in segments from roots exposed to 4 µM Cd+0.5 µM Zn (Fig.…”

Section: Discussionsupporting

confidence: 92%

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The ratio of Zn to Cd supply as a determinant of metal-homeostasis gene expression in tobacco and its modulation by overexpressing the metal exporter AtHMA4

Barabasz

Klimecka

Kendziorek

et al. 2016

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Section: Discussionsupporting

confidence: 92%

“…In tobacco their function remains largely unknown. The only up to date study was performed on NtZIP1 and suggests its involvement in Fe transport (Sano et al , 2012). The most extensive analysis has been performed on Arabidopsis.…”

Section: Discussionmentioning

confidence: 99%

The ratio of Zn to Cd supply as a determinant of metal-homeostasis gene expression in tobacco and its modulation by overexpressing the metal exporter AtHMA4

Barabasz

Klimecka

Kendziorek

et al. 2016

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“…Accordingly, here we found two copies of NtZIP5: NtZIP5A and NtZIP5B (Additional file 2). Detailed bioinformatics analysis showed that NtZIP5A has a nucleotide sequence identical with NtZIP1 previously identified by Sano et al [31], and here we provided data based on which NtZIP1 was renamed NtZIP5A.…”

Section: Isolation Of Ntzip5b and Bioinformatics Analysissupporting

confidence: 71%

“…NtZIP5A/B -a novel Zn deficiency-inducible transporter involved in Zn and Cd uptake NtZIP1 (AB505626 / NM_001325745.1) was first cloned by Sano et al [31] from tobacco BY-2 cells. However, he focused primarily on NtNRAMP1, and only an initial study on NtZIP1 was performed.…”

Section: Discussionmentioning

confidence: 99%

Zn/Cd status-dependent accumulation of Zn and Cd in root parts in tobacco is accompanied by specific expression of ZIP genes

et al. 2020

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Background: Root-to-shoot translocation of zinc (Zn) and cadmium (Cd) depends on the concentrations of both metals in the medium. A previous study on tobacco (Nicotiana tabacum) pointed to the contribution of NtZIP1, NtZIP2, NtZIP4 and NtIRT1-like in the regulation of this phenomenon. To learn more, Zn and Cd accumulation, root/ shoot distribution and the expression of ZIP genes were investigated in the apical, middle and basal root parts. Results: We show that Zn/Cd status-dependent root-shoot distribution of both metals was related to distinct metal accumulation in root parts. At low Zn and Cd in the medium, the apical part contained the highest metal level; at higher concentrations, the middle and basal parts were the major sink for excess metal. The above were accompanied by root part-specific expression pattern modifications of ZIPs (NtZIP1-like, NtZIP2, NtZIP4A/B, NtZIP5A/B, NtZIP5-like, NtZIP8, NtZIP11, NtIRT1, and NtIRT1-like) that fell into four categories with respect to the root part. Furthermore, for lower Zn/Cd concentrations changes were noted for NtZIP5A/B and NtZIP5-like only, but at higher Zn and Cd levels for NtZIP1-like, NtZIP5-like, NtZIP8, NtZIP11, NtIRT1, and NtIRT1-like. NtZIP1, here renamed to NtZIP5B, was cloned and characterized. We found that it was a zinc deficiency-inducible transporter involved in zinc and cadmium uptake from the soil solution primarily by the middle root part. Conclusions: We conclude that regulation of the longitudinal distribution of Zn and Cd is highly specific, and that the apical, middle and basal root parts play distinct roles in Zn/Cd status-dependent control of metal translocation efficiency to shoots, including the stimulation of Zn translocation to shoots in the presence of Cd. These results provide new insight into the root part-specific unique role of NtZIP5B and other ZIP genes in the longitudinal distribution of zinc and cadmium and their contribution to the regulation of root-to-shoot translocation.

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“…Study of Cailliatte et al ( 2009 ) demonstrated that Arabidopsis transgenic plants overexpressing AtNRAMP6 gene were hypersensitive to Cd, although plant Cd content remained unchanged, thereby indicating that modification in expression pattern of AtNRAMP6 affects distribution and availability of Cd within the cell. However, Sano et al ( 2012 ) have shown that Nicotiana tabacum NRAMP1 gene ( NtNRAMP1 ), a plasma membrane transporter, when overexpressed in tobacco BY-2 cells increases resistance of the cells to both Fe and Cd, and suggested that NtNRAMP1 moderates Fe-uptake and prevents toxicity resulting from excess Fe or Cd application. Tiwari et al ( 2014 ) also demonstrated that OsNRAPM1 , localized on plasma membrane of endodermis and pericycle cell, when expressed in Arabidopsis provides tolerance against As and Cd with their enhanced accumulation in root and shoot, and proposed that modification in this gene may be helpful in reducing the risk of food chain contamination by these toxic heavy metals.…”

Section: “Omics” Toolsmentioning

confidence: 99%

Heavy Metal Tolerance in Plants: Role of Transcriptomics, Proteomics, Metabolomics, and Ionomics

et al. 2016

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Heavy metal contamination of soil and water causing toxicity/stress has become one important constraint to crop productivity and quality. This situation has further worsened by the increasing population growth and inherent food demand. It has been reported in several studies that counterbalancing toxicity due to heavy metal requires complex mechanisms at molecular, biochemical, physiological, cellular, tissue, and whole plant level, which might manifest in terms of improved crop productivity. Recent advances in various disciplines of biological sciences such as metabolomics, transcriptomics, proteomics, etc., have assisted in the characterization of metabolites, transcription factors, and stress-inducible proteins involved in heavy metal tolerance, which in turn can be utilized for generating heavy metal-tolerant crops. This review summarizes various tolerance strategies of plants under heavy metal toxicity covering the role of metabolites (metabolomics), trace elements (ionomics), transcription factors (transcriptomics), various stress-inducible proteins (proteomics) as well as the role of plant hormones. We also provide a glance of some strategies adopted by metal-accumulating plants, also known as “metallophytes.”

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Metal Ion Homeostasis Mediated by NRAMP Transporters in Plant Cells - Focused on Increased Resistance to Iron and Cadmium Ion

Cited by 11 publications

References 56 publications

The ratio of Zn to Cd supply as a determinant of metal-homeostasis gene expression in tobacco and its modulation by overexpressing the metal exporter AtHMA4

The ratio of Zn to Cd supply as a determinant of metal-homeostasis gene expression in tobacco and its modulation by overexpressing the metal exporter AtHMA4

Zn/Cd status-dependent accumulation of Zn and Cd in root parts in tobacco is accompanied by specific expression of ZIP genes

Heavy Metal Tolerance in Plants: Role of Transcriptomics, Proteomics, Metabolomics, and Ionomics

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