The ratio of Zn to Cd supply as a determinant of metal-homeostasis gene expression in tobacco and its modulation by overexpressing the metal exporter AtHMA4

Barabasz, Anna; Klimecka, Maria; Kendziorek, Maria; Weremczuk, Aleksandra; Ruszczyńska, Anna; Bulska, Ewa; Antosiewicz, Danuta Maria

doi:10.1093/jxb/erw389

Cited by 33 publications

(36 citation statements)

References 40 publications

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“…dependence of the cadmium uptake upon zinc variations in soil was hardly observed. Mutual correlations between zinc and cadmium in soil and plant environment are well recognized and documented in the scientific literature 21,48 . However, the stability of the cadmium uptake upon zinc variation in soil has not been reported so far.…”

Section: Resultsmentioning

confidence: 98%

Combined cadmium-zinc interactions alter manganese, lead, copper uptake by Melissa officinalis

Adamczyk-Szabela

Lisowska

Romanowska-Duda

et al. 2020

Sci Rep

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farmland soil typical for the polish rural environment was used in pot experiment to estimate the impact of cadmium and zinc on the manganese, lead and copper uptake by lemon balm (Melissa officinalis L). Bioavailable and total forms of investigated metals in soil and metal concentrations in plants were determined by atomic absorption spectrometry. the plant photosynthesis indicators were also examined. Intensification of photosynthesis upon the high zinc and cadmium soil supplementation was observed. This effect was not detected at low metal concentrations. ANOVA proved that cadmium and zinc treatments influenced manganese, lead and copper transfer from soil and their concentration in plants. Zinc uptake and accumulation in either roots or above-ground parts in plant was inversely proportional to cadmium concentration in soil. Manganese concentration in roots decreased upon the soil supplementation with either zinc or cadmium. it suggests that the latter ions are transported via symplastic pathways and compete with manganese for similar transporters. the opposite situation was observed for lead and copper. Soil supplementation with cadmium and zinc affects manganese, lead and copper concentrations and photosynthesis intensity in lemon balm plant. the following combined interactions in either normal or stress conditions are important indicators of the migration pathways.

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Section: Resultsmentioning

confidence: 98%

Combined cadmium-zinc interactions alter manganese, lead, copper uptake by Melissa officinalis

Adamczyk-Szabela

Lisowska

Romanowska-Duda

et al. 2020

Sci Rep

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“…2d). Competitive interactions between Zn and Cd modify the root-to-shoot translocation efficiency of both metals [9,[40][41][42], however the underlying mechanisms remain unknown. Two basic processes are decisive in the regulation of metal root/shoot partitioning; (i) sequestration in root cells, which determines the availability of the metal for xylem loading, (ii) efficiency of loading into xylem [43][44][45][46][47][48].…”

Section: Discussionmentioning

confidence: 99%

“…Mineralization and determination of Zn and Cd concentrations by Atomic Absorption Spectrophotometry (AAS) were performed according to Barabasz et al [53]. To compare the efficiency of Zn and Cd root-to-shoot translocation between plants grown at the applied medium compositions, the Translocation Factor (TF) was determined as the ratio of shoot/root Zn and Cd concentrations [9].…”

Section: Determination Of Metal Concentrationsmentioning

confidence: 99%

“…There are strong competitive interactions between Zn and Cd. At the whole plant level these relationships are manifested, among others, by changes in Zn and Cd uptake and translocation to shoots in a manner dependent on the concentration of both metals in the medium [8,9]. For example, excess Zn affects the uptake and root/shoot distribution of Cd.…”

Section: Introductionmentioning

confidence: 99%

“…The focus on ZIP genes results from SSH (Suppression Subtractive Hybridization)-based identification of tobacco NtZIP1, NtZIP2, NtZIP4 and IRT1-like as genes having different expression under combinations of low to high Zn/Cd concentrations in the medium. This accompanied changes in the root/shoot partitioning of both metals [9]. Proteins from the ZIP family mediate the transport of a range of metals, including Zn and Cd, to the cytoplasm either from the extracellular space or from internal stores [16].…”

Section: Introductionmentioning

confidence: 99%

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Zn/Cd status-dependent accumulation of Zn and Cd in root parts in tobacco is accompanied by specific expression of ZIP genes

et al. 2020

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Background: Root-to-shoot translocation of zinc (Zn) and cadmium (Cd) depends on the concentrations of both metals in the medium. A previous study on tobacco (Nicotiana tabacum) pointed to the contribution of NtZIP1, NtZIP2, NtZIP4 and NtIRT1-like in the regulation of this phenomenon. To learn more, Zn and Cd accumulation, root/ shoot distribution and the expression of ZIP genes were investigated in the apical, middle and basal root parts. Results: We show that Zn/Cd status-dependent root-shoot distribution of both metals was related to distinct metal accumulation in root parts. At low Zn and Cd in the medium, the apical part contained the highest metal level; at higher concentrations, the middle and basal parts were the major sink for excess metal. The above were accompanied by root part-specific expression pattern modifications of ZIPs (NtZIP1-like, NtZIP2, NtZIP4A/B, NtZIP5A/B, NtZIP5-like, NtZIP8, NtZIP11, NtIRT1, and NtIRT1-like) that fell into four categories with respect to the root part. Furthermore, for lower Zn/Cd concentrations changes were noted for NtZIP5A/B and NtZIP5-like only, but at higher Zn and Cd levels for NtZIP1-like, NtZIP5-like, NtZIP8, NtZIP11, NtIRT1, and NtIRT1-like. NtZIP1, here renamed to NtZIP5B, was cloned and characterized. We found that it was a zinc deficiency-inducible transporter involved in zinc and cadmium uptake from the soil solution primarily by the middle root part. Conclusions: We conclude that regulation of the longitudinal distribution of Zn and Cd is highly specific, and that the apical, middle and basal root parts play distinct roles in Zn/Cd status-dependent control of metal translocation efficiency to shoots, including the stimulation of Zn translocation to shoots in the presence of Cd. These results provide new insight into the root part-specific unique role of NtZIP5B and other ZIP genes in the longitudinal distribution of zinc and cadmium and their contribution to the regulation of root-to-shoot translocation.

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Genetic Engineering for Heavy Metal/Metalloid Stress Tolerance in Plants

Hossain,

Tahjib‐Ul‐Arif,

Jahin

et al. 2023

Heavy Metal Toxicity and Tolerance in Plants

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The ratio of Zn to Cd supply as a determinant of metal-homeostasis gene expression in tobacco and its modulation by overexpressing the metal exporter AtHMA4

Abstract: HighlightModifications of endogenous metal-homeostasis traits, in particular co-ordinated regulation of NtZIP1, NtZIP4, NtIRT1-like, and NtVTL, contribute to the generation of the phenotype of AtHMA4-expressing tobacco.

Cited by 33 publications

References 40 publications

Combined cadmium-zinc interactions alter manganese, lead, copper uptake by Melissa officinalis

Combined cadmium-zinc interactions alter manganese, lead, copper uptake by Melissa officinalis

Zn/Cd status-dependent accumulation of Zn and Cd in root parts in tobacco is accompanied by specific expression of ZIP genes

Genetic Engineering for Heavy Metal/Metalloid Stress Tolerance in Plants

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