Metal–Organic Framework-Based Biosensor for Detecting Hydrogen Peroxide in Plants through Color-to-Thermal Signal Conversion

Abstract

Plant biotic or abiotic stresses, such as pathogens, mechanical damage, or high temperature, can increase intracellular H2O2 concentration, damaging proteins, lipids, and DNA. Most current H2O2 detection methods require the separation or grinding of plant samples, inducing plant stresses, and the process is complicated and time-consuming. This paper constructed a metal–organic framework (MOF)-based biosensor for real-time, remote, and in situ detection of exogenous/endogenous H2O2 in plant organs through color-to-thermal signal conversion. By simply spraying horseradish peroxidase, 2,2′-azino-bis­(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and the precursor of zeolite imidazolate frameworks-8 (ZIF-8), ZIF-8 biosensors were formed in situ on a plant root, petiole, or leaf. This biosensor could report sub-micromolar H2O2 in plants since the oxidation products, ABTS• +, emitted heat when they absorbed energy from near-infrared (NIR) light. Due to the plant’s low absorption in the NIR region, the ZIF-8 biosensor allowed for remote thermal sensing of H2O2 transport or biotic/abiotic stresses in plants with a high signal-to-noise ratio combining NIR laser and thermometer. Our biosensor can be used for the future development of plant sensors for monitoring plant signaling pathways and metabolism that are nondestructive, minimally invasive, and capable of real-time, in situ analysis.

Bio‐Inspired Antioxidant Heparin‐Mimetic Peptide Hydrogel for Radiation‐Induced Skin Injury Repair

“…ABTS reacts with potassium persulfate will form stable cationic radicals, and the addition of antioxidant substances to them will reduce the absorbance of the radicals at the maximum absorption wavelength of 734 nm. [ 25 ] Therefore, we further evaluated the in vitro antioxidant capacity of KY hydrogel by the ABTS method. The absorption spectral curve (Figure S8, Supporting Information) showed that the KY peptide significantly reduced the absorbance of the ABTS solution at 734 nm in comparison to the KF peptide.…”

Porous Resistive Heater-Supported BixSb2–xTe3 Thermoelectric Heterogeneous Catalysts for H2O2 Production

“…The produced H 2 O 2 was quantified using the HRP-ABTS assay based on the oxidation of ABTS to ABTS + by H 2 O 2 and the resulting color change caused by the emergence of visible-range absorption peaks at 418 and 645 nm (Figure S5). − The absorbance of the reference H 2 O 2 solution was used for quantitation.…”

Nanobiotechnology to advance stress resilience in plants: Current opportunities and challenges

“…They are also used to monitor rhizosphere soil health by detecting different gases, trace heavy metals, micronutrients, etc. The resulting data helps to protect crops against pathogenic diseases and boost yield by providing the right nutrition to the soil [ 65 , 66 ]. The proper utilization of nano-sensors can result in an augmented crop production thus facilitating the farmer [ 67 ].…”