Metallic Nanostructures Based on DNA Nanoshapes

Shen, Boxuan; Tapio, Kosti; Linko, Veikko; Kostiainen, Mauri A.; Toppari, J. Jussi

doi:10.3390/nano6080146

Cited by 23 publications

(19 citation statements)

References 108 publications

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“…For instance, Sakiyama-Elbert et al have designed a heparin-based system to immobilize neurotrophin-3 in the fibrin gel by non-covalent interaction to rein in the diffusion-based release of neurotrophin-3 [9]. In addition, the combination of DNA with some nanostructures has also been getting a lot of attention [12][13][14][15][16][17][18]. Although those molecules can be used as a connector for many biologics, many problems still exist, such as low specificity, low affinity, and high toxicity.…”

Section: Introductionmentioning

confidence: 99%

Aptamer Functionalized DNA Hydrogel for Wise-Stage Controlled Protein Release

et al. 2018

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With the simple functionalization method and good biocompatibility, an aptamer-integrated DNA hydrogel is used as the protein delivery system with an adjustable release rate and time by using complementary sequences (CSs) as the biomolecular trigger. The aptamer-functionalized DNA hydrogel was prepared via a one-pot self-assembly process from two kinds of DNA building blocks (X-shaped and L-shaped DNA units) and a single-stranded aptamer. The gelling process was achieved under physiological conditions within one minute. In the absence of the triggering CSs, the aptamer grafted in the hydrogel exhibited a stable state for protein-specific capture. While hybridizing with the triggering CSs, the aptamer is turned into a double-stranded structure, resulting in the fast dissociation of protein with a wise-stage controlled release program. Further, the DNA hydrogel with excellent cytocompatibility has been successfully applied to human serum, forming a complex matrix. The whole process of protein capture and release were biocompatible and could not refer to any adverse factor of the protein or cells. Thus, the aptamer-functionalized DNA hydrogel will be a good candidate for controlled protein delivery.

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Section: Introductionmentioning

confidence: 99%

Aptamer Functionalized DNA Hydrogel for Wise-Stage Controlled Protein Release

et al. 2018

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“…These features can be used in controlling chemical reactions [26][27][28], creating tunable plasmonic systems [29,30] and building carriers for drug delivery [12,[31][32][33][34][35]. Precise and addressable DNA origami can also be used in metrology and optical super-resolution imaging [36], forming crystals and nanoparticle superlattices [37,38] and creating metallic nanostructures [39]. Recently, it has also been observed that DNA origami structures are more resilient than previously understood [40] and that the mass production of DNA origami is affordable [41].…”

Section: Introductionmentioning

confidence: 99%

Dynamic DNA Origami Devices<strong> </strong>

Ijäs¹,

Nummelin²,

Shen³

et al. 2018

Preprint

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Structural DNA nanotechnology provides an excellent foundation for diverse nanoscale shapes that can be used in various bioapplications and materials research. From all existing DNA assembly techniques, DNA origami has proven to be the most robust one for creating custom nanoshapes. Since its invention in 2006, building from the bottom up using DNA has drastically advanced, and therefore, more and more complex DNA-based systems have become accessible. So far, vast majority of the demonstrated DNA origami frameworks are static by nature, but interestingly, there also exist dynamic DNA origami devices that are increasingly coming into view. In this review, we discuss DNA origami nanostructures that perform controlled translational or rotational movement triggered by predefined DNA strands, various molecular interactions and/or other external stimuli such as light, pH, temperature and electromagnetic fields. The rapid evolution of such dynamic DNA origami tools will undoubtedly have a significant impact on molecular scale precision measurements, targeted drug delivery and diagnostics, but they can also play a role in development of optical/plasmonic sensors, nanophotonic devices and nanorobotics for numerous different tasks.

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“…Importantly, DNA origami can be used to arrange different materials such as enzymes (6), metal nanoparticles (7), and carbon nanotubes (8). The authors have recently demonstrated how DNA origami shapes can be utilized in creating enzymatic nanoreactors (9) and metallic nanostructures (10), and how the structures can be efficiently positioned and anchored on different substrates (11,12).…”

Section: Introductionmentioning

confidence: 99%

Packaging DNA Origami into Viral Protein Cages

Linko

Mikkilä

Kostiainen

2018

Methods in Molecular Biology

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The DNA origami technique is a widely used method to create customized, complex, spatially well-defined two-dimensional (2D) and three-dimensional (3D) DNA nanostructures. These structures have huge potential to serve as smart drug-delivery vehicles and molecular devices in various nanomedical and biotechnological applications. However, so far only little is known about the behavior of these novel structures in living organisms or in cell culture/tissue models. Moreover, enhancing pharmacokinetic bioavailability and transfection properties of such structures still remains a challenge. One intriguing approach to overcome these issues is to coat DNA origami nanostructures with proteins or lipid membranes. Here, we show how cowpea chlorotic mottle virus (CCMV) capsid proteins (CPs) can be used for coating DNA origami nanostructures. We present a method for disassembling native CCMV particles and isolating the pure CP dimers, which can further bind and encapsulate a rectangular DNA origami shape. Owing to the highly programmable nature of DNA origami, packaging of DNA nanostructures into viral protein cages could find imminent uses in enhanced targeting and cellular delivery of various active nano-objects, such as enzymes and drug molecules.

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Metallic Nanostructures Based on DNA Nanoshapes

Cited by 23 publications

References 108 publications

Aptamer Functionalized DNA Hydrogel for Wise-Stage Controlled Protein Release

Aptamer Functionalized DNA Hydrogel for Wise-Stage Controlled Protein Release

Dynamic DNA Origami Devices<strong> </strong>

Packaging DNA Origami into Viral Protein Cages

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