Metallothionein immunostaining in adenoid cystic carcinomas of the salivary glands

Alves, Sérgio Melo; Cardoso, Sérgio Vitorino; Bernardes, Vanessa Fátima; Machado, Vinícius C.; Mesquita, Ricardo Alves; Carmo, María Auxiliadora Vieira do; Aguiar, Maria Cássia Ferreira de

doi:10.1016/j.oraloncology.2006.03.012

Cited by 14 publications

(11 citation statements)

References 25 publications

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“…The methods used for determination of MT in clinical samples are often based on relatively time-consuming and not enough sensitive immunohistochemistry [27,38,39]. There can be used also spectrometric [40-42], chromatographic [43-45] or electrochemical techniques for determination of MT [46-49].…”

Section: Introductionmentioning

confidence: 99%

Utilizing of Adsorptive Transfer Stripping Technique Brdicka Reaction for Determination of Metallothioneins Level in Melanoma Cells, Blood Serum and Tissues

Křížková

Fabrik

Adam

et al. 2008

Sensors

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In the paper we utilized the adsorptive transfer stripping differential pulse voltammetry Brdicka reaction for the determination of metallothioneins (MT) in melanoma cells, animal melanoma tissues (MeLiM miniature pig) and blood serum of patients with malignant melanoma. Primarily we attempted to investigate the influence of dilution of real sample on MT electrochemical response. Dilution of samples of 1 000 times was chosen the most suitable for determination of MT level in biological samples. Then we quantified the MT level in the melanoma cells, the animal melanoma tissues and the blood serum samples. The MT content in the cells varied within the range from 4.2 to 11.2 μM. At animal melanoma tissues (melanomas localized on abdomen, back limb and dorsum) the highest content of MT was determined in the tumour sampled on the back of the animal and was nearly 500 μg of MTs per gram of a tissue. We also quantified content of MT in metastases, which was found in liver, spleen and lymph nodes. Moreover the average MT level in the blood serum samples from patients with melanoma was 3.0 ± 0.8 μM. MT levels determined at melanoma samples were significantly (p < 0.05) higher compared to control ones at cells, tissues and blood serum.

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Section: Introductionmentioning

confidence: 99%

Utilizing of Adsorptive Transfer Stripping Technique Brdicka Reaction for Determination of Metallothioneins Level in Melanoma Cells, Blood Serum and Tissues

Křížková

Fabrik

Adam

et al. 2008

Sensors

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“…MT were shown to be expressed in normal salivary glands as well as in benign and malignant tumours mainly in cells resembling the phenotype of myoepithelial cells (Sunardhi-Widyaputra et al, 1995;Gao et al, 1997;Hecht et al, 2002;Ogawa, 2003;Alves et al, 2007). As in the case of OSCC, the majority of studies concerning the role of MT expression in salivary glands were based on immunohistochemistry (Sunardhi-Widyaputra et al, 1995;Gao et al, 1997;Ogawa, 2003;Alves et al, 2007).…”

Section: Metallothionein Expression In Normal Salivary Gland and Its mentioning

confidence: 99%

“…As in the case of OSCC, the majority of studies concerning the role of MT expression in salivary glands were based on immunohistochemistry (Sunardhi-Widyaputra et al, 1995;Gao et al, 1997;Ogawa, 2003;Alves et al, 2007).…”

Section: Metallothionein Expression In Normal Salivary Gland and Its mentioning

confidence: 99%

“…Normal salivary glands and its tumours exert a broad expression of MT-1/2 in myoepithelial cells (Sunardhi-Widyaputra et al, 1995;Gao et al, 1997;Ogawa, 2003;Alves et al, 2007, Prasad et al, 2008. In the study of Sunardhi-Widyaputra et al, performed on 21 benign and 4 malignant lesions of salivary glands, MT expression was compared with parathyroid hormone-related peptide (PTHrP) expression.…”

Section: Mt Expression In Normal Salivary Gland Tissues and Neoplasiasmentioning

confidence: 99%

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Expression of Metallothionein in Oral Cancer

Dzięgiel¹,

Puła²,

Podhorska-Okołów³

2012

Oral Cancer

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“…Considering the histopathological similarities between both tumor types, it is possible that MT is also present and participates in the tumorigenesis of MEC. Moreover, this protein has been linked to the protection of tumor cells against chemotherapy and radiation, which develop a neoplastic resistance to these treatments [10]. Recent studies confirmed that the presence of different MT isoforms, mostly metallothionein 2A (MT-2A), correlates with cell proliferation and clinicopathological behavior in some cancers [11,12].…”

Section: Introductionmentioning

confidence: 99%

Metallothionein Expression and its Influence on the In Vitro Biological Behavior of Mucoepidermoid Carcinoma

Aquime

Zampieri

Kataoka

et al. 2020

Cells

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Mucoepidermoid carcinoma (MEC) is the most common tumor in the salivary glands, often presenting with recurrence and metastasis due to its high invasive capacity. Metallothionein (MT), a zinc storage protein that supplies this element for protease activity, is probably related to mucoepidermoid carcinoma behavior. This prompted us to characterize a cell line derived from mucoepidermoid carcinoma and to correlate metallothionein expression with transforming growth factor-α (TGF-α), tumor necrosis factor-α (TNF-α) and matrix metalloproteinases (MMPs). Transcriptomic analysis and cytogenetic assays were performed to detect the expression of genes of interest and cellular chromosomal alterations, respectively. MEC cells with a depleted metallothionein 2A (MT2A) gene were subjected to Western blot to correlate metallothionein expression with growth factors and MMPs. Additionally, cells with depleted MT were subjected to migration and invasion assays. The transcriptomic study revealed reads mapped to cytokeratins 19 and AE1/AE3, α-smooth muscle actin, vimentin, and fibronectin. Cytogenetic evaluation demonstrated structural and numerical alterations, including the translocation t(11;19)(q21;p13), characteristic of MEC. Metallothionein depletion was correlated with the decreased expression of TGF-α and MMP-9, while TNF-α protein levels were augmented. Migration and invasion activity were diminished after metallothionein silencing. Our findings suggest an important role of MT in MEC invasion, through the regulation of proteins involved in this process.

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Metallothionein immunostaining in adenoid cystic carcinomas of the salivary glands

Cited by 14 publications

References 25 publications

Utilizing of Adsorptive Transfer Stripping Technique Brdicka Reaction for Determination of Metallothioneins Level in Melanoma Cells, Blood Serum and Tissues

Utilizing of Adsorptive Transfer Stripping Technique Brdicka Reaction for Determination of Metallothioneins Level in Melanoma Cells, Blood Serum and Tissues

Expression of Metallothionein in Oral Cancer

Metallothionein Expression and its Influence on the In Vitro Biological Behavior of Mucoepidermoid Carcinoma

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