Metallothionein induction and cadmium uptake in Bufo arenarum embryos following an acclimation protocol

Pérez-Coll, Cristina S.; Herkovits, Jorge; Fridman, Osvaldo; Dániel, P.; D'Eramo, José L.

doi:10.1016/s0269-7491(99)00096-2

Cited by 8 publications

(7 citation statements)

References 14 publications

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“…The decline of amphibian populations and the large number of malformations found in many geographic regions has caused increasing concern [4]. Some studies indicate that this could be related to their high susceptibility to contaminants, particularly during early life stages [5], in that this susceptibility has been reported with diverse physicochemical agents such as metals [6,7], pesticides [8,9], industrial [10] and pharmaceutical [11] chemicals, and ultraviolet B radiation [12,13]. In addition, the risk for adverse effects might be enhanced by the preference to breed in shallow, lentic, or ephemeral water bodies in which pollutants might be concentrated.…”

Section: Introductionmentioning

confidence: 99%

Stage‐dependent susceptibility to copper in Rhinella arenarum embryos and larvae

Aronzon

Sandoval

Herkovits

et al. 2011

Enviro Toxic and Chemistry

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Copper toxicity in different embryonic and larval stages of the common South American toad Rhinella arenarum was evaluated by means of continuous and 24-h pulse treatments in 12 different developmental stages. Lethal concentrations (LC) of 10, 50, and 90% of continuous treatment with Cu from early blastula (S.4), complete operculum (S.25), and hind limb bud (S.28) stages were plotted from 24 to 168 h, resulting from S.4 in a 24-h LC50 of 137 µg Cu(2+) /L and a 168-h LC50 of 19.5 µg Cu(2+) /L. This result was in agreement with pulse treatments that showed a high resistance to Cu at blastula and gastrula stages, whereas the organogenic period, between muscular response (S.18) and open mouth (S.21), was very susceptible to this metal. Continuous treatments from S.25 showed no significant differences along exposure time (168-h LC50 = 51 µg Cu(2+) /L), but in the case of S.28 toxicity increased slightly from a 24-h LC50 of 138.6 µg Cu(2+) /L to a 168-h LC50 of 104 µg Cu(2+) /L, pointing out that, although the larval period was significantly more resistant to Cu, there was also a remarkable stage-dependent susceptibility to this metal. Copper teratogenic potential was approximately two, and main adverse effects were reduced body size, axial flexure, microcephaly, acephaly, mouth malformations, agenesis of or underdeveloped gills, agenesis of or underdeveloped tail, and hydropsy. The results are discussed considering Cu toxicity mechanisms, an evolutionary perspective, and environmental protection.

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Section: Introductionmentioning

confidence: 99%

Stage‐dependent susceptibility to copper in Rhinella arenarum embryos and larvae

Aronzon

Sandoval

Herkovits

et al. 2011

Enviro Toxic and Chemistry

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“…In the present investigation, the amphibians Xenopus laevis (anuran) and Pleurodeles waltl (urodele) were used because of their ecotoxicological relevance which has already been well established in a large array of studies on biomarkers such as induction of biotransformation enzyme activities (Bekaert et al, 2002;Gauthier et al, 2004), micronucleus (Ferrier et al, 1998) or DNA adduct formation (Bekaert et al, 2002), comet induction following DNA damage (Mouchet et al, 2005a(Mouchet et al, ,b, 2006b, or teratogenic malformations (Chenon et al, 2002;Prati et al, 2002;Bonfanti et al, 2004). Moreover, even though the toxic effects of Cd on aquatic organisms have been well documented, notably in amphibians (Pérez-Coll et al, 1999;Flament et al, 2003;Mounaji et al, 2003;James et al, 2004), less or no data on genotoxic effects are, to our knowledge, available for amphibians.…”

Section: Introductionmentioning

confidence: 99%

Comparative evaluation of the toxicity and genotoxicity of cadmium in amphibian larvae (Xenopus laevis and Pleurodeles waltl) using the comet assay and the micronucleus test

Mouchet

Gauthier

Baudrimont

et al. 2007

Environmental Toxicology

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The toxic and genotoxic potential of Cadmium (CdCl(2)) were evaluated by the micronucleus test (MNT) and comet assay (CA) using amphibian larvae (Xenopus laevis and Pleurodeles waltl). Acute toxicity results showed that Cd is toxic to Xenopus larvae exposed from 2 to 50 mg/L and to Pleurodeles from 5 to 50 mg/L, depending on the nature of the water (reconstituted water containing mineral salts or mineral water MW (Volvic)). The MNT results obtained in MW showed that Cd (2 mg/L) is genotoxic to Xenopus, whereas it was not genotoxic to Pleurodeles at all concentrations tested. The CA established that the genotoxicity of Cd to Xenopus and Pleurodeles larvae depends on the concentration, the exposure times, and the comet parameters (Tail DNA, ETM, OTM, and TL). The CA and MNT results were compared for their ability to detect genotoxic effects, considering the concentrations of Cd applied and the exposure time. The CA showed Cd to be genotoxic from the first day of exposure. In amphibians, the CA appears to be a sensitive and suitable method for detecting genotoxicity such as that caused by Cd.

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“…This analysis could provide a contribution to define more accurately the concept of “low level exposure” for both experimental and environmental scenarios. In the case of this acclimation study, the copper concentration was gradually increased in order to enhance the response of amphibian embryos to copper as it was demonstrated in the case of cadmium [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

“…In fact, a decreased uptake of another heavy metal, cadmium, due to changes in the membrane permeability was associated to acclimation phenomena both in “in vitro” and “in vivo” experiments in kidney cells [ 52 ]. On the other hand, the acclimation processes seem to include the increased synthesis and accumulation of “stress proteins” [ 53 ] like heat shock proteins [ 54 ] and metallothioneins (MTs), the last ones usually associated with heavy metal exposure [ 51 , 55 ]. In the case of Bufo arenarum embryos exposed to low level Cu concentration, an increase in MTs was also reported [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Acclimation to Low Level Exposure of Copper in Bufo arenarum Embryos: Linkage of Effects to Tissue Residues

Herkovits

Pérez-Coll

2007

IJERPH

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The acclimation possibilities to copper in Bufo arenarum embryos was evaluated by means of three different low level copper exposure conditions during 14 days. By the end of the acclimation period the copper content in control embryos was 1.04 ± 0.09 μg.g−1 (wet weight) while in all the acclimated embryos a reduction of about 25% of copper was found. Thus copper content could be considered as a biomarker of low level exposure conditions. Batches of 10 embryos (by triplicate) from each acclimation condition were challenged with three different toxic concentrations of copper. As a general pattern, the acclimation protocol to copper exerted a transient beneficial effect on the survival of the Bufo arenarum embryos. The acclimation phenomenon could be related to the selection of pollution tolerant organisms within an adaptive process and therefore the persistence of information within an ecological system following a toxicological stressor.

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Metallothionein induction and cadmium uptake in Bufo arenarum embryos following an acclimation protocol

Cited by 8 publications

References 14 publications

Stage‐dependent susceptibility to copper in Rhinella arenarum embryos and larvae

Stage‐dependent susceptibility to copper in Rhinella arenarum embryos and larvae

Comparative evaluation of the toxicity and genotoxicity of cadmium in amphibian larvae (Xenopus laevis and Pleurodeles waltl) using the comet assay and the micronucleus test

Acclimation to Low Level Exposure of Copper in Bufo arenarum Embryos: Linkage of Effects to Tissue Residues

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