Metastatic capacity and differentiation in murine melanoma cell lines

Maiorana, Antonino; Cavallari, Vittorio; Maiorana, Mariarosa; Fano, Rita Adriana; Scimone, S.; Fante, Ryan J.; Garbisa, Spiridione

doi:10.1016/s0344-0338(11)80075-0

Cited by 4 publications

(5 citation statements)

References 18 publications

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“…In the only other report we have found in the literature, the volume fraction of melanosomes in another murine melanoma cell line (B16) was reported to be 0.9-1.2% (Maiorana et al, 1992) against the range of 2.34% that we found, but it should be noted that the Cloudman S91 subline that we studied, Me1 l l a , was originally cloned for its high degree of melanization.…”

Section: Discussioncontrasting

confidence: 44%

“…Stereological studies of melanosomes first appeared in 1992. The melanosome volume density (VV), surface density (&), numerical density (Nv), and mean area ( S ) were estimated to evaluate metastatic capacity and differentiation in B16 murine melanoma cells (Maiorana et al, 1992) but no data on the Vvof melanosomes were provided. Goodall et al (1994) estimated the size of melanosomes in B16 melanoma cells, but only by reference to their diameter, while the length and diameter of melanosomes from 17 tis-sues, including Cloudman S91 melanoma cells, have been reported by Hach et al (1993).…”

Section: Introductionmentioning

confidence: 99%

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Application of the New Stereological Probes to the Study of the Melanosome in Cloudman S91 Melanoma Cells

Dong

Dockery

1997

Pigment Cell Research

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The relationship between melanosome size and number and melanin content has been investigated in Cloudman S91 melanoma cells growing in vitro using both "model-based" and "design-based" stereological procedures. Cells were cultured for 4 days, harvested at daily intervals, and resin-embedded for light and electron microscopy; one aliquot of each sample of cells was assayed to determine its melanin content. By comparing their volume-weighted mean nuclear volume and their number-weighted mean nuclear volume, we have found that the nuclei of Cloudman melanoma cells form a fairly homogeneous population. The volume fraction and absolute volume of premelanosomes (VVpm, cell and Vpm) and mature melanosomes (VVmm, cell and Vmm) were all found to decrease progressively throughout the period of culture as did the number of premelanosomes (Npm) and mature melanosomes (Nmm). Whilst the volume-weighted mean volume of individual stage I and stage II premelanosomes, (VVipm), remained fairly constant at about 10 nm3, the volume of individual stage III and IV mature melanosomes showed significant variation ranging between about 13 nm3 and 32 nm3. The melanin content of the cells decreased progressively over the 4 days of culture. There were, however, considerable variations in both the average melanin content per unit volume of mature melanosomes, in the range 170-600 fg/micron3, and in the melanin content per individual mature melanosome, in the range 3-12 fg. Our findings show that stereological techniques can provide unbiased and sensitive tools for the study of the morphological basis of melanogenesis; their value will become even more evident when they are combined with techniques for the localization of melanogenic enzymes and their substrates.

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Section: Discussioncontrasting

confidence: 44%

Section: Introductionmentioning

confidence: 99%

Application of the New Stereological Probes to the Study of the Melanosome in Cloudman S91 Melanoma Cells

Dong

Dockery

1997

Pigment Cell Research

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“…(17), have been used to investigate the cell biological mechanisms of metastasis and the inhibitory action of agents on the metastasis of melanoma cells at the biochemical or the molecular levels in many laboratories. Accordingly, many characteristics associated with their different meta-static abilities have been analysed and published (1)(2)(3)(4)(5)(6). In order to further clarify the differences in the cell-and immune-biological properties between these two cell lines, we focused our study on the levels of expression of proto-oncogene c-fos, the sensitivities to LAK cells and/or IL-2 in vivo, and the components of gangliosides in vitro and in vivo.…”

Section: Analysis Of Ganglioside Components Of Cultured B16-f1 and Flmentioning

confidence: 99%

“…Cell biological properties of murine melanoma B16 variants have been extensively investigated in order to clarify the mechanisms of their different metastatic potentials (1)(2)(3)(4)(5)(6). However, there have been no definite conclusions about which properties or factors are responsible for the differences in metastatic potentials between the two cell lines.…”

Section: Introductionmentioning

confidence: 99%

Differential Cell‐ and Immuno‐Biological Properties of Murine B16‐F1 and F10 Melanomas: Oncogene c‐fos Expression, Sensitivity to LAK Cells and/or IL‐2, and Components of Gangliosides

Nakayama

Urabe

Tsuchida

et al. 1995

The Journal of Dermatology

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Differential cell- and immuno-biological properties of two murine melanoma B16 variants, B16-F1 and F10, were investigated. Studies focused on the expression of proto-oncogene c-fos, sensitivities to LAK cells and/or IL-2, and modulation of the expression of ganglioside components after treatment with IL-2. Proto-oncogene c-fos was found to be highly expressed in F10 lines by an in situ hybridization technique and also in F10 lung metastatic nests by immunofluorescent staining with anti-c-fos antibody. F1 melanomas were more sensitive to local injection of IL-2. F10 melanomas hardly responded to IL-2 treatment, but successive injections of a combination of LAK cells and IL-2 did cause prolongation of survival rates, even of F10 melanoma-burdened mice. A major component of gangliosides of both F1 and F10 melanomas was GM3. Production of GM3 in F10 melanomas treated with IL-2 for 4 days increased, and, if the treatment was continued for 7 days, minor components of gangliosides, such as GM2, GM1, and GD1a, appeared only in F1 melanomas, while the increase of production of GM3 disappeared in both melanomas. These experimental results may provide clues for additional mechanisms which allow these two murine melanoma variants to show different implantation and metastasis rates.

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“…Attention has been particularly focused on nuclear morphology, DNA content and chromatin structure [6,43]. In the context of metastatic potential, comparisons of mouse B16 melanoma cell lines revealed in highly metastatic variants an increased chromatin condensation [31], heterogeneity of DNA ploidy and an increased nuclear pleomorphism [25,26]. Moreover, fibroblasts transfected by the H-ras oncogene exhibited increased levels of chromatin condensation when compared to non-transformed cells [28,29] although it was difficult in this system to correlate directly chromatin changes, ras levels or metastatic ability [27].…”

Section: Introductionmentioning

confidence: 99%

Nuclear DNA Content and Chromatin Pattern of Rat Rhabdomyosarcoma Cell Sublines with Different Metastatic Potentials

Dufer

Poupon

Yatouji

2000

Analytical Cellular Pathology

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There is a constant need of features able to characterize potentially metastatic cells among the heterogeneous cell subpopulations which constitute a tumor. Image cytometry of metastatic tumor cells give rise to variable results, partly because of a heterogeneous origin of cells, or potential drug effects. The aim of this work was to characterize nuclear changes observed in metastatic cell clones issued in vitro from the same parental cell population The nuclear phenotypes of 6 cell sublines isolated from a rat rhabdomyosarcoma cell line and differing in their metastatic ability were evaluated by image cytometry on Feulgen‐stained preparations. Densitometric [5], geometric [3] and textural [9] features were computed from each nuclear image. For each cell subline, a metastatic score, ranging from 0 to 10, was calculated on the basis of in vitro invasivity data, by measuring the number of pulmonary metastases observed after s.c. graft of tumor cells in rats. Data obtained were compared to karyotype, growth characteristics, and oncogene expressions of cell lines. The nuclear DNA content, the chromosome numbers, the cell sublines doubling times, and the distribution of cells within the cell cycle appear unrelated with this score. On the contrary, increase in metastatic ability is accompanied by changes in chromatin pattern as assessed by textural features. Progressive increase in chromatin condensation can be observed in cell sublines with increasing metastatic score. These results were confirmed by an unsupervised multivariate partitioning of rhabdomyosarcoma cells which identified two separate subsets whose distributions within the analyzed cell lines correlate with their metastatic ability. These data suggest that, in rat rhabdomyosarcoma cell sublines, metastatic ability could be associated with nuclear morphological changes at the level of chromatin texture.

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Metastatic capacity and differentiation in murine melanoma cell lines

Cited by 4 publications

References 18 publications

Application of the New Stereological Probes to the Study of the Melanosome in Cloudman S91 Melanoma Cells

Application of the New Stereological Probes to the Study of the Melanosome in Cloudman S91 Melanoma Cells

Differential Cell‐ and Immuno‐Biological Properties of Murine B16‐F1 and F10 Melanomas: Oncogene c‐fos Expression, Sensitivity to LAK Cells and/or IL‐2, and Components of Gangliosides

Nuclear DNA Content and Chromatin Pattern of Rat Rhabdomyosarcoma Cell Sublines with Different Metastatic Potentials

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