Metastatic Potential Is Positively Correlated with Cell Surface Sialylation of Cultured Murine Tumor Cell Lines

Yogeeswaran, Ganesa; Salk, P L

doi:10.1126/science.7233237

Cited by 390 publications

(185 citation statements)

References 32 publications

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“…The proportion of sialylated galactose and N-acetyl galactose was similar in 1G8 and 1G3. This is close to that observed by Yogeeswaran and Salk (1981) for high metastatic cells, but 4 times higher than they observed for their low metastatic cells. This suggests that these differences also vary according to the model system.…”

Section: Aggregation and Disaggregation The Results Insupporting

confidence: 90%

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Morphological and metastatic murine melanoma variants: motility, adhesiveness, cell surface and in vivo properties

Clark¹,

Js²,

Sidebottom³

1987

Br J Cancer

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The results show enhanced differences in metastatic potential of tight and loose clones after selective cloning and that there may be important differences in motility and cell-substrate interactions. Foulds (1949) introduced the term tumour progression to describe the process by which some tumours become more malignant during their growth. Nowell (1976) attributed this to genetic instability, although genetic or epigenetic processes could result in the emergence of variant cells with growth advantages which differ from the main population in the expression of specific characters (Frost & Kerbel, 1983;Schimke et al., 1985).The existence within tumours of cells heterogeneous for the expression of a wide range of in vivo and in vitro characters is well documented (for reviews see Heppner, 1984;Nicolson, 1984).We have previously reported heterogeneity of clonal morphology in a metastatic melanomaxlymphocyte hybrid tumour cell line (Clark & Sidebottom, 1984). These variants grow either as tight or loose colonies and the isolation of cell lines, stable for clonal morphology, which have low or high metastatic phenotypes has been described (Clark & Sidebottom, 1984). The morphology and patterns of growth of these variants suggests a number of ways in which these cells may differ and which might account for their behavioural differences in vitro. These include intercellular and cell-substrate adhesion and motility, which in turn are dependent upon cell surface properties. Various stages of the metastatic cascade, for example local invasion or tumourcapillary endothelium adhesion are partially dependent upon intercellular and cell-substrate adhesion and motility (Weiss, 1980;Varani et al., 1980;Nicolson et al., 1986). The cell surface has an important role in these interactions and has been extensively studied for correlations with metastasis and organ colonization (Turner, 1982;Nicolson, 1984).Observation and measurement of adhesion and motility in vivo have inherent difficulties, but these characters can be investigated in vitro and some clues about their possible significance in vivo thereby gained (Briles & Kornfeld, 1978; Hart, 1979;Vollmers & Birchmeier, 1983;McCarthy et al., 1985).We have, therefore, looked at the adhesive and motile properties of tight and loose cells and also examined their cell surfaces by lectin binding to PAGE separated glycoproteins, radio-iodination of proteins and quantitation of sialic acid content. (Sidebottom & Clark, 1983). The cell lines 1G8(6F) and 1G3(4E) were selected from F87.CI.6T2 cells for tight or loose clonal morphology respectively and their in vitro and in vivo properties have been described elsewhere (Clark & Sidebottom, 1984). Cells were maintained in MEM +10% newborn calf serum (Flow) (cMEM) and subcultured when confluent; they were regularly tested for mycoplasma and found to be negative. Wiscm cells were derived from explanted hearts from one day old Wistar rats and were maintained in DMEM + 10% foetal calf serum (Gibco) +10% tryptose phosphate broth (Difco). To prod...

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Section: Aggregation and Disaggregation The Results Insupporting

confidence: 90%

“…Between 15 and 25% of sialic acid was released by neuraminidase from both high and low metastatic cells, which is similar to some other high metastatic lines (Dennis et al, 1982;Yogeeswaran & Salk, 1981). The proportion of sialylated galactose and N-acetyl galactose was similar in 1G8 and 1G3.…”

Section: Aggregation and Disaggregation The Results Insupporting

confidence: 81%

Morphological and metastatic murine melanoma variants: motility, adhesiveness, cell surface and in vivo properties

Clark¹,

Js²,

Sidebottom³

1987

Br J Cancer

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“…Alterations of protein N-glycosylation have been associated with some carcinogenic traits, such as invasiveness and metastatic potential. [20][21][22] Deregulated enzymatic activities of proteins directly involved in N-glycosylation or the availability of potential glycosylation sites determined by the branching of N-glycans are considered to be crucial for these effects. [23][24][25][26] In the current report, we describe the epigenetic regulation of TUSC3 expression through promoter methylation in ovarian cancer and define TUSC3 as an independent prognostic factor in 2 large, independent cohorts of women with ovarian cancer.…”

Section: Introductionmentioning

confidence: 99%

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confidence: 99%

Methylation status of TUSC3 is a prognostic factor in ovarian cancer

Pils

Horak

Vaňhara

et al. 2012

Cancer

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BACKGROUND. Current prognostic information in ovarian cancer is based on tumor stage, tumor grade, and postoperative tumor size. Reliable molecular prognostic markers are scarce. In this article, the authors describe epigenetic events in a frequently deleted region on chromosome 8p22 that influence the expression of tumor suppressor candidate 3 (TUSC3), a putative tumor suppressor gene in ovarian cancer. METHODS. Messenger RNA expression and promoter hypermethylation of TUSC3 were studied in ovarian cancer cell lines and in tumor samples from 2 large, independent ovarian cancer cohorts using polymerase chain reaction-based methods. RESULTS. The results indicated that TUSC3 expression is decreased significantly because of promoter methylation in malignant ovarian tumors compared with benign controls. Almost 33% of ovarian cancer samples had detectable TUSC3 promoter methylation. Furthermore, methylation status of the TUSC3 promoter had a significant and independent influence on progression-free and overall survival. CONCLUSIONS. TUSC3 hypermethylation predicted progression-free and overall survival in ovarian cancer. The current observations suggested a role for N-glycosylating events in ovarian cancer pathogenesis in general and identified the epigenetic silencing of TUSC3 as a prognostic factor in this disease. Cancer 2013;119:946-54. V C 2012 American Cancer Society.KEYWORDS: TUSC3, methylation, ovarian cancer, glycosylation, progression-free survival, overall survival, biomarker.. INTRODUCTIONEpithelial ovarian cancer is the most lethal gynecologic malignancy and the fourth most frequent cause of cancer-related death among women in industrialized countries. 1 Because >75% of women are diagnosed with advanced disease (International Federation of Gynecology and Obstetrics [FIGO] stages III and IV), an early diagnosis presents 1 of the challenges of this disease. Patients with advanced ovarian cancer undergo intensive multimodal therapy consisting of cytoreductive surgery and (neo)adjuvant platinum-based and taxane-based chemotherapy. Regardless of the progress made in surgical and medical therapies over recent decades, the outcome for women with advanced ovarian cancer remains grim. Innovative, hypothesis-driven approaches to biomarker development remain essential even in the era of high-throughput sequencing and various omics. Studies of promoter methylation patterns in tumor suppressor genes have yielded several promising methylated biomarker candidates. [2][3][4] In contrast to a gene or protein expression analysis, methylation can easily be detected using polymerase chain reaction (PCR)-based methods in both tumor material and body fluids. [5][6][7] TUSC3 (tumor suppressor candidate 3), originally named N33, was identified as a potential tumor suppressor gene in prostate cancer 8,9 and is located on chromosome band 8p22. Known homozygous deletions of this region in pancreatic cell lines 10,11 and prostate cancer cell lines 12,13 contain no other cancer-related genes except TUSC3, although mutations of the T...

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“…1,2) In the case of ovarian tumors, a high peritoneal dissemination potential is frequently used as an indicator of malignancy. Once the tumor cells are dispersed in the abdominal cavity, they form solid tumors that are disseminated widely in the mesenterium and the peritoneum, making treatment more difficult.…”

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confidence: 99%

Selection of Human Ovarian Carcinoma Cells with High Dissemination Potential by Repeated Passage of the Cells in vivo into Nude Mice, and Involvement of Le^x‐determinant in the Dissemination Potential

Kiguchi

Iwamori

Mochizuki

et al. 1998

Japanese Journal of Cancer Research

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Metastatic Potential Is Positively Correlated with Cell Surface Sialylation of Cultured Murine Tumor Cell Lines

Cited by 390 publications

References 32 publications

Morphological and metastatic murine melanoma variants: motility, adhesiveness, cell surface and in vivo properties

Morphological and metastatic murine melanoma variants: motility, adhesiveness, cell surface and in vivo properties

Methylation status of TUSC3 is a prognostic factor in ovarian cancer

Selection of Human Ovarian Carcinoma Cells with High Dissemination Potential by Repeated Passage of the Cells in vivo into Nude Mice, and Involvement of Le^x‐determinant in the Dissemination Potential

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Metastatic Potential Is Positively Correlated with Cell Surface Sialylation of Cultured Murine Tumor Cell Lines

Cited by 390 publications

References 32 publications

Morphological and metastatic murine melanoma variants: motility, adhesiveness, cell surface and in vivo properties

Morphological and metastatic murine melanoma variants: motility, adhesiveness, cell surface and in vivo properties

Methylation status of TUSC3 is a prognostic factor in ovarian cancer

Selection of Human Ovarian Carcinoma Cells with High Dissemination Potential by Repeated Passage of the Cells in vivo into Nude Mice, and Involvement of Lex‐determinant in the Dissemination Potential

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Selection of Human Ovarian Carcinoma Cells with High Dissemination Potential by Repeated Passage of the Cells in vivo into Nude Mice, and Involvement of Le^x‐determinant in the Dissemination Potential