Selection of Human Ovarian Carcinoma Cells with High Dissemination Potential by Repeated Passage of the Cells in vivo into Nude Mice, and Involvement of Le^x‐determinant in the Dissemination Potential

Alterations in the glycolipid composition and cellular properties of ovarian carcinoma‐derived RMG‐1 cells on transfection of the α1,2‐fucosyltransferase gene

“…(17,18) With regard to resistance against anticancer drugs, similar results to the authors' have been obtained for rat colon carcinoma cells transfected with either the α1,2-fucosyltransferase gene or its antisense sequence; the H-2 antigen, which was increased in the transfectants, was reported to be involved in the drug resistance. (19) However, our results did not reveal the possible involvement of the H-2 antigen (IV 2 Fuc-nLc 4 Cer), the concentration of which was not increased by transfection.…”

HER2 Is Frequently Over‐expressed in Ovarian Clear Cell Adenocarcinoma: Possible Novel Treatment Modality Using Recombinant Monoclonal Antibody against HER2, Trastuzumab

“…Culture conditions of human ovarian CCA cell lines, RMG-1, HAC-II and KK Cell lines KK, 16) which was kindly provided by Dr. H. Kikuchi, Department of Obstetrics and Gynecology, National Defense Medical College, RMG-1, 17) which was kindly provided by Dr. S. Nozawa, Department of Obstetrics and Gynecology, Keio University, and HAC-II, 18) which was also kindly provided by Dr. M. Nishida, Department of Obstetrics and Gynecology, University of Tsukuba, were cultured in Dulbecco's modified Eagle's medium (DMEM, Nissui, Tokyo) supplemented with 10% fetal calf serum (Mitsubishi Chemicals Co., Tokyo). All of these cell lines were proved to be resistant to known anti-cancer drugs in our previous study.…”

Enhancive effects of Lewis y antigen on CD44-mediated adhesion and spreading of human ovarian cancer cell line RMG-I

“…Ovarian cancer mostly has changes of type II glycosylated antigens, such as Lewis x, Lewis y and H antigens, which mainly depend on the α1, 2-FT-catalyzed fucosylation of galactose residues at the non-reducing terminal [19]. Our previous study showed that ovarian cancer cell line RMG-I mainly expressed Lewis × antigen, and confirmed that the enhanced adhesion of Lewis × antigen-overexpressed cells to peritoneal mesothelia was weakened after Lewis × antigen blocking in nude mouse experiments, suggesting that Lewis × antigen is related to the intraperitoneal dissemination of RMG-I cells [20]. We transfected wild type α1,2-FT gene into ovarian cancer cell line RMG-I to establish the α1,2-FT-overexpressed cell line RMG-I-H, and found that the activity of α1,2-FT in RMG-I-H cells was enhanced by 20 to 30 times[5].…”