Methadone ameliorates multiple-low-dose streptozotocin-induced type 1 diabetes in mice

Amirshahrokhi, Keyvan; Dehpour, Ahmad Reza; Hadjati, Jamshid; Sotoudeh, Masoud; Ghazi‐Khansari, Mahmoud

doi:10.1016/j.taap.2008.06.020

Cited by 45 publications

(37 citation statements)

References 44 publications

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“…CT-1 appears to protect mice from STZ-induced diabetes in vivo, as Ct1 −/− mice show higher susceptibility to develop diabetes and greater loss in insulin content upon STZ-induced damage compared with WT mice based on both acute [32] and chronic [33] diabetic mouse models. Ct1 −/− mice also present mild glucose intolerance compared with WT mice, accompanied by reduced insulin secretion in response to the glucose load (measured 10 min after injection), although glycaemia was also normalised at later time points in Ct1 −/− mice.…”

Section: Discussionmentioning

confidence: 99%

Cardiotrophin 1 protects beta cells from apoptosis and prevents streptozotocin-induced diabetes in a mouse model

et al. 2013

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Aims/hypothesis Cardiotrophin 1 (CT-1) is a recently described cytokine originally isolated from the heart where it has been shown to play an important role in apoptotic protection of cardiomyocytes and heart hypertrophy. Its beneficial properties have also been described in other organs such as liver and neuromuscular tissue. In the present study, we investigated whether CT-1 can confer protection against pro-apoptotic stimuli in pancreatic beta cells, and its role in insulin secretion and diabetes development.Methods The effects of CT-1 on apoptosis and function were studied using MIN6B1 cells and freshly isolated murine pancreatic islets. The impact on the development of diabetes was evaluated in Ct1-null (Ct1 −/− ) mice (the gene Ct1 is also known as Ctf1) using two streptozotocin (STZ)-induced models of diabetes. Results CT-1 has a protective effect in MIN6B1 cells and murine islets under the pro-apoptotic stimulus of serum deprivation, which correlates with the expression of B cell lymphoma-extra large, or following exposure to a mixture of cytokines. In addition, CT-1 enhances glucose-stimulated insulin secretion in MIN6B1 cells and this was repressed by inhibitors of phospholipase C. Furthermore, Ct1 −/− mice were more prone to develop diabetes, and their glucose tolerance test showed impaired plasma glucose clearance which correlated with decreased pancreatic insulin secretion. Conclusions/interpretation The results obtained from both in vitro and in vivo experiments show that CT-1 improves beta cell function and survival, and protects mice against STZ-induced diabetes.

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Section: Discussionmentioning

confidence: 99%

Cardiotrophin 1 protects beta cells from apoptosis and prevents streptozotocin-induced diabetes in a mouse model

et al. 2013

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“…Faktor kedua adalah penurunan berat badan pada keadaan diabetes membutuhkan waktu sampai dengan masa kompensasi kekurangan energi belum tercapai pada rentang waktu penelitian ini. Bila tahap ini akan tercapai maka kekurangan energi ini menyebabkan terjadinya proses glukoneogenesis masive di hepar, sehingga menyebabkan hilangnya masa otot [19].…”

Section: Hewan Cobaunclassified

“…Selanjutnya perubahan gula darah akan diawali dengan peningkatan gula darah setelah dilakukan injeksi STZ. Peningkatan ini dapat menjadi indikasi keadaan diabetes bila gula darah terperiksa  200 mg/dl [19].…”

Section: Kadar Gula Darah Hewan Cobaunclassified

Kadar NF- Kβ Pankreas Tikus Model Type 2 Diabetes Mellitus dengan Pemberian Tepung Susu Sapi

Nugroho¹,

Ginting

Diana

2015

IJHN

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AbstrakType 2 Diabetes Mellitus dihubungkan dengan kerusakan sel pankreas yang mempengaruhi jumlah dan fungsi insulin penderitanya. NF-K merupakan golongan protein dalam faktor transkripsi yang diduga memiliki peran penting dalam pro-apoptosis sel pankreas. NF-K merupakan pemicu pro-dan anti-apotosis sel beta pankreas dengan kecenderungan proapoptosis lebih besar dibandingkan peran anti-apoptosisnya. Vitamin D dari susu sapi bubuk adalah vitamin dengan kemampuan menghambat aktifitas NF-Kβ, melalui proses perbaikan sensitivitas dan produksi insulin, serta peningkatan pertahanan sel pankreas. Penelitian ini bertujuan untuk mengukur kekuatan efek pemberian susu sapi bubuk terhadap penurunan NF-K pankreas tikus model type 2 diabetes. Selama 90 hari penelitian, tikus dibagi menjadi 5 kelompok, terdiri dari 2 kelompok kontrol (negative = non-diabetic rat dan positive = diabetic rat) dan 3 kelompok perlakuan (P1, P2 dan P3) dengan dosis pemberian tepung susu sapi adalah 0,9 g/hr, 1,8 g/hr dan 2,7 g/hr. Pada akhir penelitian kadar NF-K pankreas tikus diukur menggunakan metode Elisa. Hasil uji Mann Whitney U terhadap kadar NF-K kelompok perlakuan dibandingkan kontrol positif menunjukkan tidak ditemukan perbedaan (p value = 1,000; 0,086; 0,248). Efek penurunan kadar NF-K yang diharapkan dari pemberian susu sapi bubuk pada keadaan diabetes tidak terbukti. Sehingga disimpulkan pemberian susu sapi bubuk tidak dapat digunakan untuk mencegah kerusakan sel pankreas melalui mekanisme penurunan NF-K.

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“…Hanya mencit dengan kadar glukosa darah ≥200 mg/dL yang digunakan dalam penelitian ini (Amirshahrokhi et al 2008).…”

Section: Penentuan Dosis Induksi Streptozotocinunclassified

“…Berdasarkan hasil penelitian Amirshahrokhi et al (2008), induksi STZ dengan dosis 65 mg/kg BB menghasilkan hewan percobaan model diabetes autoimun yang mirip dengan DM tipe-1 pada manusia. Hal ini dibuktikan dengan adanya infiltrasi sel-T dan makrofag pada sel pulau langerhans akibat kerusakan yang total secara permanen dari sel β-pankreas.…”

Section: Pembahasanunclassified

The effect of daun sendok (Plantago major) extract on the blood glucose level of Balb/C mice induced by streptozotocin

2010

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Pengaruh pemberian ekstrak herba daun sendok (Plantago major) terhadap kadar glukosa darah mencit Balb/C induksi streptozotocin . This research aimed to determine the effect of daun sendok (Plantago major L.) extract on the blood glucose level of Balb/C mice induced by streptozotocin. This study was a laboratory experimental pre and post-test control group design. The subjects used were 16 male mice. Then, mice were induced with streptozotocin by a dose of 65 mg/kg body weight intraperitoneally freshly dissolved in 0.02 M citrate saline buffer. Mice used were mice with blood glucose levels ≥200 mg/dL. Furthermore, mice were divided into 2 groups by a simple random sampling. Group 1 was given metformin in a dose of 1.3 mg/mice/day and group 2 was given daun sendok extract in a dose of 1000 mg/kg body weight/day. At the 2 nd week, it was measured for the glucose levels from the blood of mice tail by a Blood Glucose Stick Meter (Gluco DrTM). The data obtained were statistically analyzed by independent samples t-test by using SPSS Programme for Microsoft Windows release 17.0. The significance was set at p<0.05. The difference of average blood glucose levels pre vs post experiment, metformin group was -145.87 mg/dL and daun sendok group was -85.00 mg/dL (p=0.024). The experiment results showed that daun sendok extract in a dose of 1000 mg/kg body weight/day could reduce the blood glucose levels in Balb/C mice induced by streptozotocin.

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Methadone ameliorates multiple-low-dose streptozotocin-induced type 1 diabetes in mice

Cited by 45 publications

References 44 publications

Cardiotrophin 1 protects beta cells from apoptosis and prevents streptozotocin-induced diabetes in a mouse model

Cardiotrophin 1 protects beta cells from apoptosis and prevents streptozotocin-induced diabetes in a mouse model

Kadar NF- Kβ Pankreas Tikus Model Type 2 Diabetes Mellitus dengan Pemberian Tepung Susu Sapi

The effect of daun sendok (Plantago major) extract on the blood glucose level of Balb/C mice induced by streptozotocin

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