Methane overtone absorption by intracavity laser spectroscopy

Window into the mind: Advanced handheld spectroscopic eye-safe technology for point-of-care neurodiagnostic

“…Figure 1B shows the representative Raman spectra of murine brain tissue measured using an excitation wavelength of 633 nm spanning the fingerprint and high-frequency regions, from a commercial Raman instrument (Qontor InVia Renishaw). We observe an apparent enhancement of high–wave number bands, associated with the resonance of the methylene overtone at 619.68 nm ( 38 ), which normally yield relatively weak peaks with the 785-nm excitation. In addition to the strong high–wave number response, these bands suffer little interference from fluorescence, which often tends to dominate the fingerprint region.…”

“…Membranes of cells are the primary target for injury and their damage and are highly dependent on their physical properties and lipid organization, affecting membrane fluidity, which is a key property for maintaining cell functionality and depends on lipid composition and cell environment, leading to distortions, deformations, and decrease of mechanical stability (57)(58)(59). Cardiolipin undergoes oxidation during the pathophysiological cascade in TBI, with an accumulation of similar oxidation products in the region of injury (38,60). A further link has been established between the spectral changes and apoptosis via comparison to immunohistochemistry of TBI in mice using Raman spectroscopy (33).…”