Methanobactin, a Copper-Acquisition Compound from Methane-Oxidizing Bacteria

Kim, Hyung J.; Graham, David W.; DiSpirito, Alan A.; Alterman, Michail A.; Galeva, Nadezhda A.; Larive, Cynthia K.; Asunskis, D.J.; Sherwood, Peter M. A.

doi:10.1126/science.1098322

Cited by 316 publications

(378 citation statements)

References 29 publications

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“…Furthermore, Fe can also be stored within cells inside proteins, such as ferritins, for retrieval in times of external Fe stress (or to compartmentalize the Fe during dormancy to protect it from reacting with O 2 , thereby generating products potentially toxic to DNA). There are no such sequestering or storage systems yet known for copper in bacteria with the exception of some methanotrophic bacteria that excrete Cu-chelating compounds [62]. Hence, copper availability to the cell depends on the concentrations of Cu in the local external environment as well as on its state of chelation within soils.…”

Section: Nitrous Oxide Emissions From Soilsmentioning

confidence: 99%

Biological sources and sinks of nitrous oxide and strategies to mitigate emissions

Thomson

Giannopoulos

Pretty

et al. 2012

Phil. Trans. R. Soc. B

433

282

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Nitrous oxide (N 2 O) is a powerful atmospheric greenhouse gas and cause of ozone layer depletion. Global emissions continue to rise. More than two-thirds of these emissions arise from bacterial and fungal denitrification and nitrification processes in soils, largely as a result of the application of nitrogenous fertilizers. This article summarizes the outcomes of an interdisciplinary meeting, ‘Nitrous oxide (N 2 O) the forgotten greenhouse gas’, held at the Kavli Royal Society International Centre, from 23 to 24 May 2011. It provides an introduction and background to the nature of the problem, and summarizes the conclusions reached regarding the biological sources and sinks of N 2 O in oceans, soils and wastewaters, and discusses the genetic regulation and molecular details of the enzymes responsible. Techniques for providing global and local N 2 O budgets are discussed. The findings of the meeting are drawn together in a review of strategies for mitigating N 2 O emissions, under three headings, namely: (i) managing soil chemistry and microbiology, (ii) engineering crop plants to fix nitrogen, and (iii) sustainable agricultural intensification.

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Section: Nitrous Oxide Emissions From Soilsmentioning

confidence: 99%

Biological sources and sinks of nitrous oxide and strategies to mitigate emissions

Thomson

Giannopoulos

Pretty

et al. 2012

Phil. Trans. R. Soc. B

433

282

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“…In that study, however, appropriate standards containing Cu(I) or Cu(II) bound to N,S ligands were not examined by XPS. 14 To further investigate the copper oxidation state in methanobactin, we collected Cu K edge XAS data on Cu(II)-loaded samples. The XANES spectrum (Figure 2, top) clearly indicates the presence of Cu(I), as shown by the presence of a 1s → 4p transition at 8985 eV and edge features resembling four coordinate cuprous models.…”

mentioning

confidence: 99%

The Copper Chelator Methanobactin from Methylosinus trichosporium OB3b Binds Copper(I)

et al. 2005

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The oxidation state of copper bound to methanobactin, a small siderophore-like molecule from the methanotroph Methylosinus trichosporium OB3b, was investigated. Purified methanobactin loaded with Cu(II) exhibits a weak EPR signal probably due to adventitious Cu(II). The EPR signal intensity increases significantly upon addition of the strong oxidant nitric acid. Features of the X-ray absorption near edge spectrum, including a 1s → 4p transition at 8985 eV, further indicate the presence of Cu(I). EXAFS data were best fit using a multiple scattering model generated from previously reported crystallographic parameters. These results establish definitively that M. trichosporium OB3b methanobactin binds Cu(I) and suggest that methanobactin itself reduces Cu(II) to Cu(I).

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“…Two copper-uptake systems have been observed in M. capsulatus, one is through methanobactin; a copper-binding, siderophore-like, chromopeptide that is produced a number of methanotrophs [29]- [32] and the other via the outer membrane protein; MopE [33], [34] Both of them presumably contribute to copper homeostasis in M. capsulatus, however, their interaction with CopA has yet to be investigated. To our knowledge this is the first study investigating the disruption of a P-type ATPase from M. capsulatus.…”

Section: ) Bioinformatic Analyses and Protein Topology Of Copa Protementioning

confidence: 99%

Mutagenesis of a Copper P-Type ATPase Encoding Genein Methylococcus capsulatus (Bath) Results inCopper-Resistance

Khalifa¹

2013

IJBBB

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Abstract-Copper is an essential micronutrient for all living cells, however, it is extremely toxic at high concentrations and thus copper homeostasis is required to be tightly regulated. copA encodes for a copper-translocating P-type ATPase (CopA) and plays a vital role in copper homeostasis and is involved in copper transport across membranes of many organisms. Little is known about copper homeostasis in Methylococcus capsulatus although copper has a significant physiological role in this methanotroph. In this study we investigated the disruption of a CopA1 homologue (MCA2072; copA1) in M. capsulatus (Bath) by insertional inactivation mutagenesis. The resulting mutant, M. capsulatus ΔcopA1, was copper resistant to elevated copper concentrations (100 µM) than the wild-type strain (80 µM). Furthermore, the intracellular copper measurements revealed that ΔcopA1 accumulated half the amount of copper when compared with the wild-type. No observed phenotypic difference between the mutant strain and wild-type related to growth at different silver concentrations. These observations suggest that M. capsulatus CopA1 has a key role in copper homeostasis.

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Methanobactin, a Copper-Acquisition Compound from Methane-Oxidizing Bacteria

Cited by 316 publications

References 29 publications

Biological sources and sinks of nitrous oxide and strategies to mitigate emissions

Biological sources and sinks of nitrous oxide and strategies to mitigate emissions

The Copper Chelator Methanobactin from Methylosinus trichosporium OB3b Binds Copper(I)

Mutagenesis of a Copper P-Type ATPase Encoding Genein Methylococcus capsulatus (Bath) Results inCopper-Resistance

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