Methanogenic community during the anaerobic digestion of different substrates and organic loading rates

Kong, Dewang; Zhang, Keqiang; Ji, Liang; Gao, Wenxuan; Du, Lei

doi:10.1002/mbo3.709

Cited by 26 publications

(10 citation statements)

References 40 publications

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“…Archaeoglobus and Pyroccoccus were identified only in treated wastewater (S5). This result described above was consistent with the findings from literature [ 32 , 33 ].…”

Section: Resultssupporting

confidence: 94%

“…Also, Methanosaeta , Methanococcus and Methanobrevibacter were detected with lower average values 4.1%, 3.4% and 2.9%, respectively. Methanosarcina and Methanosaeta are well-known for utilizing acetate for methanogenesis while Methanospirillium , Methanoculleus and Methanobrevibacter are hydrogenotrophic methanogens [ 32 ]. Both, acetoclastic and hydrogenotrophic pathways are popular among the methanogens.…”

Section: Resultsmentioning

confidence: 99%

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Insights into the microbial diversity and structure in a full-scale municipal wastewater treatment plant with particular regard to Archaea

et al. 2021

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Due to limited description of the role and diversity of archaea in WWTPs, the aim of the study was to analyze microbial community structures and diversities with particular regard to Archaea in the samples taken from different stages of the full-scale municipal wastewater treatment plant and effluent receiving water (upstream and downstream discharge point). Our study was focused on showing how the treatment processes influenced the Eubacteria and Archaea composition. Alpha and Beta diversity were used to evaluate the microbial diversity changes in the collected samples. Proteobacteria was the largest fraction ranging from 28% to 67% with 56% relative abundance across all samples. Archaea were present in all stages of WWTP ranged from 1 to 8%. Among the Archaea, two groups of methanogens, acetoclastic (Methanosarcina, Methanosaeta) and hydrogenotrophic methanogens (Methanospirillium, Methanoculleus, Methanobrevibacter) were dominant in the technological stages. The obtained results indicate that the treated wastewater did not significantly affect eubacterial and archaeal composition in receiving water. However, differences in richness, diversity and microbial composition of Eubacteria and Archaea between the wastewater samples taken from the primary and secondary treatment were observed.

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“…Archaeoglobus and Pyroccoccus were identified only in treated wastewater (S5). This result described above was consistent with the findings from literature [ 32 , 33 ].…”

Section: Resultssupporting

confidence: 94%

Section: Resultsmentioning

confidence: 99%

Insights into the microbial diversity and structure in a full-scale municipal wastewater treatment plant with particular regard to Archaea

et al. 2021

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“…By contrast, Methanosarcinales was only marginally inhibited, in that its copies increased to 9.44 × 10 6 copies/g TS, thus suggesting acetotrophic methanogenesis became the dominant bioconversion pathway in the final OLR. During the digestions of pig manure, maize straw, and mixture feedstock, significant shifts in the methanogen community also occurred, with the OLR increasing to 4 g/(L⋅d), but the hydrogenotrophic and aceticlastic methanogens were only found in approximately equal contents for the digestion of maize straw ( Kong et al, 2018 ). Its corollary is that the reduced hydrogen produced during the hydrolysis process may not be used by methanogens in a timely manner, causing propionate to accumulate in the reactor.…”

Section: Resultsmentioning

confidence: 99%

Applying EEM- PARAFAC Analysis With Quantitative Real-Time PCR to Monitor Methanogenic Activity of High-Solid Anaerobic Digestion of Rice Straw

Deng

Ruan

et al. 2021

Front. Microbiol.

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The methanogenic activity is an important indicator to assess the efficiency of high-solid anaerobic digestion. However, it is not yet elucidated clearly how to detect the parameter rapidly and reliably in the rice straw feeding reactor. Co-inoculated with ruminal digesta and anaerobic sludge, the digestion performance was studied at three different organic loading rates (OLRs). The excitation emission matrix–parallel factor analysis (EEM–PARAFAC) was used to detect dynamic changes in the characteristic of fluorescence components. Our results revealed that CH4 productivity reached 280.90 mL/g volatile solid (VS) with a 54.39% CH4 content under the OLR of 2.26 g/(L⋅d), which amount to 80.29% of its theoretical value. At the OLR of 2.47 g/(L⋅d), the average accumulated NH4+ concentration was 1082.63 mg/L, which resulted in the hydrogenotrophic Methanobacteriales decreasing from 1.70 × 109 to 1.04 × 106 copies/g in the solid residues, whereas the acetotrophic Methanosarcinales increased from 7.89 × 106 to 9.44 × 106 copies/g. The dynamics of the methanogenic community consequently influenced the bioconversion efficiency of rice straw, and CH4 productivity was reduced to 256.54 mL/g VS. The three fluorescent components, at the excitation/emission wavelength of 420 nm/470 nm, 340 nm/430 nm, and 280 nm/340 nm, were decomposed by PARAFAC model in the digestate. Fluorescence intensities of coenzyme F420 and NADH reflected the dynamic changes of CH4-producing activity and anaerobic digestion efficiency, respectively. The coenzyme F420, unique to hydrogenotrophic methanogens, was correlated with methane yield, suggesting they played a dominant role in the anaerobic reactor. This study demonstrates that the EEM–PARAFAC combined with Q-PCR can be used to characterize methanogenic activity variation during the high-solid anaerobic digestion of rice straw with 15% total solid (TS).

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“…AD is a biological process widely used for converting organic materials into biogas through a series of tandem biochemical reactions, including hydrolysis, acidogenesis, acetogenesis, syntrophic acetate oxidation, homoacetogenesis, and methanogenesis (Li et al, 2019; Liebetrau et al, 2019). Among the various parameters that affect AD system stability, such as pH (Logan et al, 2021), organic loading rate (OLR) (Kong et al, 2019), C/N ratio (Xue et al, 2020) and hydraulic retention time (HRT) (Liu et al, 2018), temperature is an important factor that profoundly affects the metabolism of the microbial community and subsequently influences the efficiency and stability (Nie et al, 2021). These effects can be reflected on cumulative gas production intuitively.…”

Section: Introductionmentioning

confidence: 99%

Influence of organic loading rate and temperature fluctuation caused by solar energy heating on food waste anaerobic digestion

2022

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Anaerobic digestion, one of the most currently remarkable techniques for biogas production, has provided a method of high organic solid waste disposal. Operating temperature, especially in the winter of northern city, makes biomass degradation less efficient. The microorganisms that take on the role of gas production are greatly affected by temperature. In our study, solar energy was selected for anaerobic digestion and winter was selected as the experimental environment. Anaerobic digestion was performed with solar heating and electric heating separately. Parameters were tested (pH, soluble chemical oxygen demand, total ammonia nitrogen, total volatile fatty acids), and microbial structure was monitored. The volume of methane produced was measured over 60 days. The methane yield differed by 15.92% under different conditions. It is clearly shown that methane yield can be improved by a steady temperature environment. Nevertheless, dominant bacteria and microbial structure did not seem to be much different. This study may provide more energy-saving ideas for winter anaerobic digestion projects in northern regions.

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Methanogenic community during the anaerobic digestion of different substrates and organic loading rates

Cited by 26 publications

References 40 publications

Insights into the microbial diversity and structure in a full-scale municipal wastewater treatment plant with particular regard to Archaea

Insights into the microbial diversity and structure in a full-scale municipal wastewater treatment plant with particular regard to Archaea

Applying EEM- PARAFAC Analysis With Quantitative Real-Time PCR to Monitor Methanogenic Activity of High-Solid Anaerobic Digestion of Rice Straw

Influence of organic loading rate and temperature fluctuation caused by solar energy heating on food waste anaerobic digestion

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