Methanol extract from Vietnamese Caesalpinia sappan induces apoptosis in HeLa cells

Hung, Tran Manh; Dang, Nguyen Hai; Dat, Nguyen Tien

doi:10.1186/0717-6287-47-20

Cited by 14 publications

(9 citation statements)

References 24 publications

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“…Our results were agreed with the previous reports. The extract and isolated compounds, especially phenolic compounds, from C. sappan showed an ability to inhibit the growth of several cancer cells [13,23,24]. Therefore, this study presents that brazilin (1) could be a potential anticancer property against leukemia cells and reveals the first analysis of isolated compounds from C. sappan with cytotoxicity on leukemic cell lines.…”

Section: Discussionmentioning

confidence: 99%

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Phytochemical and Cytotoxic Investigations of the Heartwood of Caesalpinia Sappan Linn

Ngernnak

Panyajai

Anuchapreeda

et al. 2018

Asian J Pharm Clin Res

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Objective: The purpose of this study was to identify the chemical constituents in the dichloromethane extract by gas chromatography-mass spectrometry (GC-MS) analysis and evaluate the cytotoxic effects on leukemia cells of isolated compounds from Caesalpinia sappan Linn.Methods: Dichloromethane extract obtained from the heartwood of C. sappan was investigated by GC-MS and column chromatography. Cytotoxic effects on leukemia cells of the isolated compounds were examined using MTT assay. Results:The GC-MS analysis of dichloromethane extract from C. sappan revealed the presence of 14 compounds. Linoleic acid and β-sitosterol were found to be the major compounds presenting in 14% and 13%, respectively. The separation of the dichloromethane extract led to the isolation of brazilin (1) as a major compound, together with lupeol (2), and a mixture of β-sitosterol (3), and stigmasterol (4). Their structures were elucidated based on spectroscopic methods. Brazilin (1) showed a cytotoxic effect on human acute myeloid leukemia cell (KG1) and human acute myeloid leukemia stem cell (KG1a) with inhibitory concentration at 50% growth (IC 50 ) values of 13.30 ± 0.49 and 12.24 ± 1.08 µg/ml, respectively. Conclusion:Many groups of phytochemical compounds in the dichloromethane extract of C. sappan were detected by GC-MS analysis. Some of them have been reported to possess various biological activities. Moreover, brazilin (1) isolated compound from C. sappan shows cytotoxicity on leukemia cells, which could be a potential anticancer property.

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Section: Discussionmentioning

confidence: 99%

“…Among these compounds, brazilin (1), a homoisoflavonoid, showed the most cytotoxic effect on both leukemic cell lines. Hung et al [23] revealed that the methanol extract of C. sappan exhibited cytotoxic activity against several of the cancer cell lines.…”

Section: Discussionmentioning

confidence: 99%

Phytochemical and Cytotoxic Investigations of the Heartwood of Caesalpinia Sappan Linn

Ngernnak

Panyajai

Anuchapreeda

et al. 2018

Asian J Pharm Clin Res

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“…Caspase-3 activation is needed for some typical characteristic of apoptosis and is absolutely neccesary for apoptotic chromatin condensation, DNA fragmentation, and finally is one of the determinants in cancer growth and development. 17 Cancer research on microenvironment, especially the extracellular matrix is arising as a key points bringing great impact on cancer cells progression. MMPs are shown in almost all human cancers.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic and MMPs inhibitory activities of Sappan Wood (Caesalpiniasappan L.): various extracts on 4T1 breast cancer cell line

Haryanti

Widiyastuti

Rahmawati

2018

hsji

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Latar belakang: Kayu secang (Caesalpinia sappan L.) merupakan salah satu tanaman potensial dengan berbagai khasiat obat, termasuk antikanker. Gelatinase (MMP-2 dan MMP-9) merupakan matriks metaloproteinase (MMPs) dan memiliki peran penting dalam inisiasi, invasi, dan metastasis kanker. Penelitian ini bertujuan untuk menguji aktivitas sitotoksik dan penghambatan MMP dari ekstrak kayu secang pada sel kanker payudara 4T1. Metode: Serbuk kayu secang dibagi menjadi 4 bagian, masing-masing diekstraksi dengan pelarut berbeda. Etanol 96%, etanol 70%, dan metanol digunakan untuk metode maserasi, sedangkan air untuk metode infusa. Uji MTT digunakan untuk menentukan efek sitotoksisitas, dan uji gelatin zymography untuk mendeteksi aktivitas MMP-2 dan MMP-9. Profil fitokimia ekstrak diamati dengan Kromatografi Lapis Tipis (KLT). Hasil: Profil fitokimia ekstrak air menunjukkan profil KLT yang berbeda, sementara tiga ekstrak lainnya memiliki profil yang sama. Ekstrak etanol 96% kayu secang memberikan efek sitotoksik paling kuat terhadap 4T1 dengan nilai IC50 13,1 μg/mL, diikuti metanol (21,4 μg/mL), etanol 70% (22,5 μg/mL) dan air (25,5 μg/mL). Analisis gelatin zymograph dengan software ImageJ menunjukkan bahwa semua ekstrak kecuali air, menghambat aktivitas gelatinolitik MMP-9 Kesimpulan: Ekstrak kayu secang dengan berbagai pelarut polar memiliki aktivitas sitotoksik dan penghambatan MMP pada sel kanker payudara 4T1 dan berpotensi untuk dikembangkan sebagai anti kanker payudara. Kata kunci: Caesalpinia sappan, MMP, sitotoksik, 4T1 Abstract Background: Sappan wood (Caesalpinia sappan L.) is one of potential plant with wide variety of medicinal properties, including anticancer. Gelatinases (MMP-2 and MMP-9) are the member of matrix metalloproteinases (MMPs) and having a key role in cancer initiation, invasion, and metastasis. This study evaluated the cytotoxic and MMPs inhibitory activities of sappan wood in various extract on 4T1 breast cancer cell lines. Methods: Sappan wood powder were divided into 4 parts, each part was extracted with different solvent. Ethanol 96%, ethanol 70%, and methanol were used for maceration methods, while water for infusion method. MTT assay used to identify cytotoxicity effect, and gelatin zymography assay to detect the activity of MMP-2 and MMP-9. Phytochemical profiling of the extract were observed by Thin Layer Chromatography (TLC). Results: Phytocemical profiling of water extract showed different TLC profile, while three others had similar profile. The results of MTT assay showed that ethanolic 96% extract exhibited the strongest cytotoxic effect against 4T1 with the IC50 value 13,1 μg/mL, followed by methanolic (21,4 μg/mL), ethanolic 70% (22,5 μg/mL) and water (25,5 μg/mL). The analysis of gelatin zymograph bands using ImageJ software proved that all extracts except water, inhibited gelatinolytic activity of MMP-9. Conclusion: The results of the study suggest that various extract of the sappan wood have been found to posses cytotoxic and MMPs inhibitory activities. Keywords: Caesalpinia sappan, MMP’s, cytotoxic, 4T1

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“…By having this IC 50 value, both extracts have a relatively strong cytotoxic effect and could be further explored as an anti-cancer agent. Effect of sappan wood extract on HeLa cell give IC 50 value on 26.50 ug/mL (Hung, et al, 2014). Another study of C. sappan ethanolic extract give IC50 value of 37 ug/mL on MCF-7 cells (Khamsita, et al, 2012) and 25 uM/mL on MCF7/HER2 cells (Rahmawaty, et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Study in the effect of F. septica ethanolic extract with concentration of 6 µg/mL could induce MCF7 cells into apoptosis through downregulation of BCl2 (Sekti, et al, 2010). Hung, et al (2014) reported that apoptosis in HeLa cell could be induced by methanolic extract of C. sappan with IC 50 value of 26.50 µg/mL. *Corresponding author e-mail: meiyan_e@ugm.ac.id…”

Section: Introductionmentioning

confidence: 99%

Different 4T1 Cells Migration under Caesalpinia sappan L. and Ficus septica Burm.f Ethanolic Extracts

Haryanti

Murwanti

Putri

et al. 2017

IJCC

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Secang (C. sappan L.) and awar (F. septica Burm.f) are known of Indonesian traditional medicine that highly consumed throughout centuries in order to cure various diseases. Recently, researchers also concern about its effects as anti-cancer on various cell types. This study was conducted to understand the ethanolic extract of C. sappan L. (ECS) and F. septica Burm.f (EFS) effects on 4T1 cells migration at various concentrations. Firstly, we examine cell proliferation profile with MTT assay under treatment with the extracts and obtained the IC50 value of ECS (20 μg/mL) and EFS (15 μg/mL). Subsequent assay conducted was to examine the cells migration under low concentration resulting in the migration inhibitory effect of both EFS and ECS with different intensity. EFS performed better migration inhibitory effect than ECS. Finally, we conducted gelatin zymography and western blot and revealed that the migration inhibitory effect of EFS may correlate to the lowering of protein expression of MMP9 and Rac-1 after 24 hours of treatment. We conclude that both extracts are potential to be developed as anticancer agent and EFS is more potent for anti-metastasis.

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Methanol extract from Vietnamese Caesalpinia sappan induces apoptosis in HeLa cells

Cited by 14 publications

References 24 publications

Phytochemical and Cytotoxic Investigations of the Heartwood of Caesalpinia Sappan Linn

Phytochemical and Cytotoxic Investigations of the Heartwood of Caesalpinia Sappan Linn

Cytotoxic and MMPs inhibitory activities of Sappan Wood (Caesalpiniasappan L.): various extracts on 4T1 breast cancer cell line

Different 4T1 Cells Migration under Caesalpinia sappan L. and Ficus septica Burm.f Ethanolic Extracts

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