Methenamine silver staining quantitative digital histochemistry in chronic allograft nephropathy

Sarıoğlu, Sülen; Celik, Ali; Şakar, Mustafa; Sönmez, Devrim; Tekis, D.

doi:10.1016/j.transproceed.2004.10.043

Cited by 11 publications

(17 citation statements)

References 10 publications

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“…Images were processed with MEDISCOPE IMAGE ANALYSIS software (Mediscope, Dokuz Eylül University, Clinical Engineering Department, İzmir, Turkey) as previously described [21]. In each case, ten high-power fields were selected, and acquisition and digitalization were performed.…”

Section: Tissue Preparationmentioning

confidence: 99%

“…The system then selected the areas with the same degree and configuration of staining and determined the area of selection as a percentage of the whole cortical region within each field, including any structural component, glomeruli, tubule, interstitium or vascular structure. The mean of ten fields was determined and denoted the stained area percentage (SAP) [21,22].…”

Section: Tissue Preparationmentioning

confidence: 99%

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Protective effect of Epo on oxidative renal injury in rats with cyclosporine nephrotoxicity

et al. 2008

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The aim of our study was to determine the effect of recombinant human erythropoietin (rhEPO) on cyclosporine (CsA) nephrotoxicity. Twenty-six female Wistar rats were injected with 15 mg/kg subcutaneous CsA and intraperitoneal saline/rhEPO for 28 days. Four groups were formed: Group 1 (n = 5), a control group; Group 2 (n = 7), CsA + saline; Group 3 (n = 7), CsA + low dose (20 U/kg per day) rhEPO; Group 4 (n = 7), CsA + high dose (100 U/kg per day) rhEPO. Body weights, creatinine clearance, urinary protein/creatinine, hematocrit, serum creatinine levels, histopathological parameters, apoptosis and lipid peroxidation tests were compared between the three groups. Body weights and renal functions were similar in Groups 2, 3 and 4 rats but significantly lower than the values found for the control group at the end of the study. The hematocrit was significantly different between the four groups, showing a positive association with the strength of the injected rhEPO doses. Tubular and arteriolar damage was significantly lower in Groups 3 and 4 rats than in Group 2 rats, while chronic changes were similar between the three groups. TUNEL-positive cells and thiobabarbituric acid reacting substance (TBARS) levels were significantly higher in Group 2 rats, whereas superoxide dismutase levels were significantly lower in Group 2 rats than in those of the other three groups. Low or high dose rhEPO had no significant protective effects on body weight, renal functions, chronic fibrotic changes, but both doses reduced tubular and arteriolar changes, apoptotis and oxidative stress.

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Section: Tissue Preparationmentioning

confidence: 99%

Section: Tissue Preparationmentioning

confidence: 99%

Protective effect of Epo on oxidative renal injury in rats with cyclosporine nephrotoxicity

et al. 2008

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“…Additionally, H. pylori was searched in every section (10,11,12). In the sections of the whole gastric mucosa, painted hematoksilen eosin was processed and researched.…”

Section: Microscopic Evaluation Of Gastric Mucosamentioning

confidence: 99%

“…The stained mucosal areas in the captured images were quantified by measuring the high power fields using a software (Mustafa Sakar, ‹zmir, Turkey). The magenta color was selected; the image analysis program calculated the stained area percentage (SAP), as described previously (11)(12)(13). On the image when the magenta color was selected, neighbors to that and designated color neighbors were covered by the analysis programme automatically, and was expressed as the proportion of the selected area to the total area ( Fig.…”

Section: Determination Of Gastric Mucus By Quantitative Digital Histomentioning

confidence: 99%

A study on the protective activity of kefir against gastric ulcer

Orhan

Karagözlü

Sarıoğlu³

et al. 2012

Turk J Gastroenterol

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“…Images were processed with Mediscope Image Analysis Software (Mediscope, Dokuz Eylul University, Clinical Engineering Department, Izmir, Turkey) (Kavukcu et al 2003;Demiral et al 2004;Sarioglu et al 2004;Sis et al 2005). For each case, after detection of the most severely scarred areas by eye, 5 high power fields were selected for digital acquisition (total area: 79,390 μ m 2 ).…”

Section: Image Analysismentioning

confidence: 99%

Glutamine Administration Enhances the Healing of Lung Parenchymal Injuries and Reduces Air Leakage in Rats

Şanli

Önen

Sarıoğlu

et al. 2006

Tohoku J. Exp. Med.

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Beneficial effects of glutamine on wound healing are well known. Parenchymal injuries in the lung cause air leakage that resolves with wound healing. We aimed to determine the effect of glutamine on the healing of lung injuries. Wistar albino female rats were randomized in three groups. One group (control, n = 7) received intraperitoneal injection of 0.9% sodium chloride (1.5 ml /day), while other group (GLN, n = 7) received glutamine (1.5 g/kg/day), beginning two days prior to the operation for total four days. After thoracotomy, a lung parenchymal lesion was made with a scalpel in the right upper lobe. Only thoracotomy was performed to sham group (n = 4). Air leakage was observed in the isolated lungs of control group, but not GLN and sham groups, at 5 cm H 2 O of positive airway pressure ( p < 0.001). The threshold of positive airway pressure for air leakage was 4.85 ± 0.37 and 19.42 ± 4.54 cm H 2 O for control and GLN groups, respectively ( p < 0.001). For measurement of collagen content in the healing parenchyma, digital images were processed to calculate the stained area percentage (SAP). SAP for immature collagen, a marker for wound healing, was 0.36 ± 0.18% and 1.48 ± 0.83% ( p = 0.02) in control and GLN groups, respectively, but no significant difference was noted in SAP for mature collagen. The grade of inflammation was not significantly different between control and GLN groups. We conclude that glutamine enhances lung parenchymal healing by increasing immature collagen secretion.glutamine; collagen; air leakage; lung

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Methenamine silver staining quantitative digital histochemistry in chronic allograft nephropathy

Cited by 11 publications

References 10 publications

Protective effect of Epo on oxidative renal injury in rats with cyclosporine nephrotoxicity

Protective effect of Epo on oxidative renal injury in rats with cyclosporine nephrotoxicity

A study on the protective activity of kefir against gastric ulcer

Glutamine Administration Enhances the Healing of Lung Parenchymal Injuries and Reduces Air Leakage in Rats

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