Methicillin resistance of staphylococci isolated from the skin of dogs with pyoderma

Kania, Stephen A.; Williamson, Nicola; Frank, Linda A.; Wilkes, Rebecca P.; Jones, Rebekah D.; Bemis, David A.

doi:10.2460/ajvr.2004.65.1265

Cited by 95 publications

(108 citation statements)

References 16 publications

(19 reference statements)

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“…The frequency of MRSP detected in the present study (94.3%) was much higher than those observed in similar studies (Medleau et al, 1986;Kania et al, 2004;Sasaki et al, 2007;Griffeth et al, 2008;Kawakami et al, 2010), which leads to an important concern in Veterinary Medicine in Brazil. Kawakami, et al (2010) also found a high percentage of MRSP isolates (66.5%) derived from dogs with pyoderma through the investigation of the mecA gene.…”

Section: Discussioncontrasting

confidence: 54%

“…The Staphylococcus genus is characterized by its easy development of antimicrobial resistance (Guardabassi et al, 2004;Weese and van Duijkeren, 2010 Methicillin-resistant S. aureus (MRSA) and methicillin-resistant S. pseudintermedius (MRSP) are of great concern in veterinary medicine and public health (Weese and van Duijkeren, 2010). In dogs with pyoderma, the occurrence of MRSP may vary between 0 and 66% (Medleau et al, 1986;Kania et al, 2004;Sasaki et al, 2007;Griffeth et al, 2008;Kawakami et al, 2010).…”

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confidence: 99%

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Description of Methicillin-resistant Staphylococcus pseudintermedius from canine pyoderma in Minas Gerais state, Brazil

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Corsini

et al. 2016

Arq. Bras. Med. Vet. Zootec.

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Methicillin-resistant Staphylococcus pseudintermedius (MRSP) is of worldwide concern in veterinary medicine. The identification of resistant strains is necessary for proper treatment and the prevention of its propagation among animals. This study aimed to identify S. pseudintermedius isolated from canine pyoderma and evaluate their resistance profiles. Lesions from 25 dogs with pyoderma were sampled. Bacterial isolates were subjected to phenotypic and genotypic analysis for identification of the causative agent. S. pseudintermedius isolates were subjected to SmaI macrorestriction analysis and PFGE for genetic grouping, and PCR to identify the presence of the mecA gene. Their resistance profiles against 12 antimicrobials were also assessed. According to the microbiological analysis, 70 of the 75 isolates obtained were S. pseudintermedius. The isolates presented PFGE patterns, with similarity varying between 84.6 and 100%, and were grouped into 19 clusters. Despite a high frequency of mecA-positive isolates (66 out 70), only 12 presented resistances to oxacillin. Multi-resistance was identified in 29 isolates. The high frequency of MRSP isolated in this study highlights the relevance of identifying resistant strains to lead proper clinical treatment.Keywords: Staphylococcus pseudintermedius, canine pyoderma, mecA, PFGE, antimicrobial resistance RESUMO Staphylococcus pseudintermedius meticilina-resistente (MRSP) é de preocupação mundial na medicina veterinária. A identificação de cepas resistentes é necessária a um tratamento adequado e à prevenção da

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Section: Discussioncontrasting

confidence: 54%

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confidence: 99%

Description of Methicillin-resistant Staphylococcus pseudintermedius from canine pyoderma in Minas Gerais state, Brazil

Bourguignon

Viçosa

Corsini

et al. 2016

Arq. Bras. Med. Vet. Zootec.

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“…The mecA gene sequences found in S. pseudintermedius are essentially identical to those found in methicillin-resistant S. aureus, 14 and penicillin binding protein 2a has been demonstrated by immunologic methods in methicillin-resistant S. pseudintermedius (MRSP). 4,11 It is presumed that expression of mecA in S. pseudintermedius has the same potential to confer broad clinical resistance to all beta-lactam drugs (penicillins, cephalosporins, carbapenems) as it does in S. aureus and other Staphylococcus species; however, this requires further study.…”

Section: Discussionmentioning

confidence: 69%

Evaluation of Susceptibility Test Breakpoints Used to Predict mecA-Mediated Resistance in Staphylococcus Pseudintevmedius Isolated from Dogs

et al. 2009

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Abstract. Clinical and Laboratory Standards Institute interpretive breakpoints for in vitro susceptibility tests that predict mecA-mediated oxacillin resistance in Staphylococcus pseudintermedius isolates from animals have been changed twice in the past decade. Moreover, there are no counterpart recommendations for human isolates of S. pseudintermedius. Individual medical and veterinary laboratories variably use interpretive breakpoints identical to those recommended for use with Staphylococcus aureus or identical to those recommended for use with coagulase-negative staphylococci. The purpose of the current study was to examine correlations between oxacillin disk diffusion, oxacillin gradient diffusion, oxacillin microbroth dilution, and cefoxitin disk diffusion tests used to predict mecA-mediated resistance in S. pseudintermedius and to retrospectively estimate, from disk diffusion zone diameter measurements, the prevalence and rate of increase of oxacillin resistance among canine S. pseudintermedius isolates submitted to a veterinary teaching hospital laboratory. Oxacillin disk diffusion zone diameters of #17 mm and oxacillin minimum inhibitory concentrations of $0.5 mg/ml were highly correlated with detection of mecA in canine S. pseudintermedius isolates by polymerase chain reaction. MecA-mediated resistance among S. pseudintermedius isolates from dogs increased from less than 5% in 2001 to near 30% in 2007. More than 90% of the methicillin-resistant S. pseudintermedius isolates in 2006 and 2007 were also resistant to representatives of $4 additional antimicrobial drug classes. Cefoxitin disk diffusion with the resistance breakpoint set at #24 mm significantly underestimated the presence of mecA in S. pseudintermedius.

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“…mecA encodes an altered penicillinbinding protein 2a (PBP2a) (FRANK & LOEFFLER, 2012) and confers resistance to all beta-lactam antibiotics, including penicillins, cephalosporins, and carbapenems (KANIA et al, 2004; VAN DUIJKEREN et al, 2011;PRIYANTHAet al, 2016). In addition, methicillin-resistant S. pseudintermedius (MRSP) strains are commonly resistant to several other classes of non-beta-lactam antibiotics, including aminoglycosides, quinolones, macrolides, phenicols, sulfonamides, and tetracyclines (PAPICH, 2012).…”

Section: Introductionmentioning

confidence: 99%

Frequency and antimicrobial susceptibility of Staphylococcus pseudintermedius in dogs with otitis externa

et al. 2018

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Infections by Staphyloccocus pseudintermedius in the skin of dogs have been object of studies, since such microorganisms often present multiple resistance to antibiotics. This study aimed to identify and evaluate the antimicrobial susceptibility of Staphylococcus pseudintermedius (SP) strains isolated from dogs with otitis. Swabs from both ears of 52 dogs with otitis (n=104) were included. Bacteria were cultured using Muller-Hinton agar (supplemented with 5% equine blood and incubated at 37°C for 24 to 48 hours. All colonies underwent biochemical evaluation for identification of staphylococci. The identity of colonies as SP was confirmed by polymerase chain reaction. The antimicrobial susceptibility of SP strains was evaluated by disk diffusion. The presence of the gene mecA was evaluated in all SP isolates by PCR. Forty-four SP strains were isolated from swabs of 31 dogs (31/52, 59.6%). Seventy-five percent of the strains were susceptible to cephalexin and 93.2% to amoxicillin plus clavulanic acid. Less than 23% of the strains were susceptible to penicillin. For non-beta-lactam antimicrobials, 63.6% of the strains showed resistance to sulfamethoxazole-trimethoprim, 61.4% to tetracycline, and 38, 64% to enrofloxacin. Aminoglycoside resistance rate was 27.3% for gentamicin. Resistance to oxacillin in vitro was detected in 13 of the 44 strains (29, 55%). A total of 12 strains (27.3%) were positive for mecA gene and five of these 12 strains were susceptible to in vitro oxacillin. Twenty-six (59, 1%) strains were resistant to three or more classes of antimicrobials, and classified as multi resistant. Our results showed high frequency of SP and multi resistant isolates to antimicrobials commonly used in veterinary.

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Methicillin resistance of staphylococci isolated from the skin of dogs with pyoderma

Abstract: The latex agglutination assay for the detection of PBP2a expression coupled with the PCR assay for the mecA gene may provide new information about emerging antimicrobial resistance among staphylococcal isolates.

Cited by 95 publications

References 16 publications

Description of Methicillin-resistant Staphylococcus pseudintermedius from canine pyoderma in Minas Gerais state, Brazil

Description of Methicillin-resistant Staphylococcus pseudintermedius from canine pyoderma in Minas Gerais state, Brazil

Evaluation of Susceptibility Test Breakpoints Used to Predict mecA-Mediated Resistance in Staphylococcus Pseudintevmedius Isolated from Dogs

Frequency and antimicrobial susceptibility of Staphylococcus pseudintermedius in dogs with otitis externa

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