Methicillin-resistant food-related Staphylococcus aureus: a review of current knowledge and biofilm formation for future studies and applications

Doulgeraki, Agapi I.; Ciccio, Pierluigi Aldo Di; Ianieri, Adriana; Nychas, George‐John E.

doi:10.1016/j.resmic.2016.08.001

Cited by 105 publications

(88 citation statements)

References 182 publications

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“…In addition, PIA expression is repressed by tcaR, a transcriptional regulator of the teicoplanin-associated locus. The protein regulator of biofilm formation augments ica gene expression, PIA production and biofilm formation (37–39). In addition, Pamp et al (40) demonstrated that Spx, a global regulator of stress response genes, inhibits biofilm formation, potentially by modulating icaR.…”

Section: Discussionmentioning

confidence: 99%

Preliminary study on the effect of brazilin on biofilms of Staphylococcus aureus

et al. 2018

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Biofilms significantly enhance antibiotic resistance by inhibiting penetration of antibiotics and are shielded from the immune system via the formation of an extracellular polymeric matrix. Innovative and novel approaches are required for the inhibition of biofilm formation and treatment of biofilm-associated infectious diseases. In the current study, a biofilm model of Staphylococcus aureus was established in vitro to explore inhibitory effects of brazilin (BN) on biofilm formation and to evaluate damaging effects of BN in the presence and absence of vancomycin (VCM) on the biofilm. Antibiofilm-infection mechanisms of BN were observed. In these experiments, the clinical strain of S. aureus C-4-4 was isolated for biofilm formation. Crystal violet staining and fluorescence microscopy revealed that BN inhibited biofilm formation in vitro and the best effect was observed with two times the minimum inhibitory concentration of BN following 48 h incubation. Additionally, the results demonstrated that BN in combination with VCM enhanced the damage to biofilms, whereas VCM alone did not. The results of the reverse transcription-quantitative polymerase chain reaction analyses demonstrated that BN downregulated gene expression of intercellular adhesion (ica)A and upregulated icaR and the quorum-sensing (QS) system regulator accessory gene regulator A. In summary, BN inhibited S. aureus biofilm formation and destroyed biofilms, while simultaneously increasing permeability to VCM. BN was able to reduce production of the extracellular polymeric matrix and inhibited the QS system. These results support the use of BN as a novel drug and treatment strategy for S. aureus biofilm-associated infections.

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Section: Discussionmentioning

confidence: 99%

Preliminary study on the effect of brazilin on biofilms of Staphylococcus aureus

et al. 2018

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“…Recently, there is an increasing report of human colonization and infection with LA-MRSA. Even though this is commonly reported among veterinarians and other personnel working in close contact with animals, LA-MRSA colonization of individuals without prior contact with animals have been reported (Doulgeraki et al, 2017). The major clonal complex commonly shared between animals and humans is the CC 398 which was predominantly reported in pigs.…”

Section: Acquisition Of Resistances Determinants and The Evolution Ofmentioning

confidence: 99%

“…Host colonization provides the basis for initiation of disease process, MRSA are frequent colonizers of the nostrils (Gordon and Lowy, 2008;Doulgeraki et al, 2017). Nasal carriage contributes to dissemination of MRSA to other body parts, this was evident when topical mupirocin was used to treat nasal colonization of MRSA which was followed by a subsequent decolonization of other body parts.…”

Section: Acquisition Of Resistances Determinants and The Evolution Ofmentioning

confidence: 99%

“…Thus, indicating the persistent nature of the HA-MRSA in the health care setting. The formation of biofilms by some strains of MRSA deficient in agr was also observed to have exacerbated the magnitude of infection within the biofilm matrix; MRSA can withstand the therapeutic effect of some antibiotics and also evade the host defence system by adhering to indwelling catheters, implants, and prosthetic heart devices through the formation of biofilm (Liu, 2009;Doulgeraki et al, 2017). Virulent species of MRSA causes severe disease, which can be fatal (DeLeo FR et al, 2010) with a high morbidity and mortality.…”

Section: Advances In Animal and Veterinary Sciencesmentioning

confidence: 99%

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Dissemination of Resistance and Virulence Determinants in Methicillin-Resistant Staphylococcus aureus During Colonization and Disease: A Review

Bitrus¹,

Zunita²,

Goni³

et al. 2018

Adv. Anim. Vet. Sci.

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“…Earlier reports have clearly shown that food contamination by Staphylococci was due to the equipment and surfaces on which food is prepared (Gibson et al, 1999) and more recently, the presence of MRSA was confirmed in different retailed meat products (Kamal et al, 2013). However, the most common way for contamination of food with Staphylococcus is through contact with food workers who carry the bacteria or through contaminated milk and cheeses and/or contaminated food not properly refrigerated (Doulgeraki et al, 2017). Staphylococcal toxins can cause nausea, vomiting, stomach cramps, and diarrhea.…”

Section: Introductionmentioning

confidence: 99%

Effect of Rocket (Eruca sativa) Extract on MRSA Growth and Proteome: Metabolic Adjustments in Plant-Based Media

et al. 2017

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The emergence of methicillin-resistant Staphylococcus aureus (MRSA) in food has provoked a great concern about the presence of MRSA in associated foodstuff. Although MRSA is often detected in various retailed meat products, it seems that food handlers are more strongly associated with this type of food contamination. Thus, it can be easily postulated that any food could be contaminated with this pathogen in an industrial environment or in household and cause food poisoning. To this direction, the effect of rocket (Eruca sativa) extract on MRSA growth and proteome was examined in the present study. This goal was achieved with the comparative study of the MRSA strain COL proteome, cultivated in rocket extract versus the standard Luria-Bertani growth medium. The obtained results showed that MRSA was able to grow in rocket extract. In addition, proteome analysis using 2-DE method showed that MRSA strain COL is taking advantage of the sugar-, lipid-, and vitamin-rich substrate in the liquid rocket extract, although its growth was delayed in rocket extract compared to Luria–Bertani medium. This work could initiate further research about bacterial metabolism in plant-based media and defense mechanisms against plant-derived antibacterials.

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Methicillin-resistant food-related Staphylococcus aureus: a review of current knowledge and biofilm formation for future studies and applications

Cited by 105 publications

References 182 publications

Preliminary study on the effect of brazilin on biofilms of Staphylococcus aureus

Preliminary study on the effect of brazilin on biofilms of Staphylococcus aureus

Dissemination of Resistance and Virulence Determinants in Methicillin-Resistant Staphylococcus aureus During Colonization and Disease: A Review

Effect of Rocket (Eruca sativa) Extract on MRSA Growth and Proteome: Metabolic Adjustments in Plant-Based Media

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