Methicillin-resistant &lt;i&gt;Staphylococcus aureus&lt;/i&gt; isolates from Iranian restaurant food samples: Panton-Valentine Leukocidin, SCCmec phenotypes and antimicrobial resistance

Ranjbar, Reza; Shahreza, Mohammad Hossein Sakhaei; Rahimi, Ebrahim; Jonaidi-Jafari, Nematollah

doi:10.4314/tjpr.v16i8.26

Cited by 10 publications

(7 citation statements)

References 19 publications

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“…Most of them reported the con rmation of phenotypic pattern of antibiotic resistance by the presence of diverse antibiotic encoding genes [16,66,76,77] High distribution of blaZ, mecA, tetK, aacA-D, msrA, ermA, gyrA, dfrA1, and blaCTX-M antibiotic resistance encoding genes in the S. aureus bacteria isolated from diverse kinds of food samples and also human clinical infections have been reported from Iran [66,76,78], India [79], Georgia [72], Nigeria [80], Germany [81], Egypt [82], and Switzerland [83]. Comparable to our research, higher prevalence of msrA than msrB [66,76,78,84], ermA than ermB [66,76,78,84,85], tetK than tetM [66,76,78,84] vatA than vatB [66,76,78,84], vanA than vanB [66,76,78,84,85] and gyrA than grlA [66,76,84] antibiotic resistance encoding genes has been reported in recent years. Our ndings were also disclosed a higher prevalence of phenotypic pro le of resistance than genotypic pro le.…”

Section: Discussionsupporting

confidence: 76%

Antimicrobial Resistance Properties of Staphylococcus Aureus Isolates From Powdered Packaged Medicinal Plants and Bottle Herbal Distillates

Mashak

Tavakoli‐Far

2020

Preprint

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BackgroundHuman involvement in the production and processing of medicinal plants and herbal distillates caused a potential risk of microbial contamination, particularly with Staphylococcus aureus. The present research was performed to assess the prevalence and phenotypic and genotypic properties of antibiotic resistance of S. aureus bacteria isolated from diverse kinds of powdered packaged medicinal plant and bottle herbal distillate samples. MethodsThree-hundred different powdered packaged medicinal plant and bottle herbal distillate samples produced in traditional conditions were collected and examined by the culture method. Phenotypic and genotypic patterns of antibiotic resistance of S. aureus isolates were examined using disk diffusion and PCR techniques. ResultsThirty out of three-hundred (10%) powdered packaged medicinal plant and bottle herbal distillate samples were contaminated with S. aureus. The prevalence of S. aureus amongst the powdered packaged medicinal plant and bottle herbal distillate samples were 8.33% and 11.11%, respectively. A. citrodora (10%) and R. damascene (10%) powdered packed medicinal plants and A. maurorum (16.66%) bottle herbal distillate had the highest contamination rate with S. aureus. S. aureus isolates harbored the highest prevalence of resistance toward penicillin (93.33%), tetracycline (90%), gentamicin (86.66%), erythromycin (70%), trimethoprim-sulfamethoxazole (63.33%) and ciprofloxacin (53.33%). Totally, 13.33% of the S. aureus isolates harbored resistance toward more than 7 antibiotic agents. blaZ (63.33%), tetK (60%), ermA (46.66%), msrA (43.33%), aacA-D (43.33%), and mecA (43.33%) were the most frequent antibiotic resistance genes. ConclusionsPowdered packaged medicinal plant and bottle herbal distillate samples may be sources of multidrug resistant-S. aureus, which poses a hygienic threat concerning the consumption of these therapeutic options in Iran. Nevertheless, further research is compulsory to understand other epidemiological features of S. aureus in powdered packaged medicinal plant and bottle herbal distillate samples.

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Section: Discussionsupporting

confidence: 76%

Antimicrobial Resistance Properties of Staphylococcus Aureus Isolates From Powdered Packaged Medicinal Plants and Bottle Herbal Distillates

Mashak

Tavakoli‐Far

2020

Preprint

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“…Our ndings addressed the higher distribution of tetK (80.55%) than tetM (66.66%) antibiotic resistance genes. Similarly, higher incidence of tetK than tetM antibiotic resistance genes was also reported previously [5,6,8,10,40,46,50,51]. Higher incidence of antibiotic resistance genes reported in some previously published papers is due to the fact that they examined the distribution of antibiotic resistance genes amongst the methicillin-resistant S. aureus (MRSA) strains.…”

Section: Discussionsupporting

confidence: 68%

“…These genes are mainly encoded resistance against quinupristindalfopristin and streptogramin antibiotic agents. R Ranjbar, MHS Shahreza, E Rahimi and N Jonaidi-Jafari [50]reported that the total incidence of vatA, vatB and vatC antibiotic resistance genes amongst the S. aureus strains isolated from foods with animal origins were 38.55%, 19.27% and 9.63%, respectively.…”

Section: Discussionmentioning

confidence: 99%

Molecular typing, phenotypic and genotypic assessment of antibiotic resistance and virulence factors amongst the Staphylococcus aureus bacteria isolated from raw chicken meat

Fazel

Karimi

Momtaz

2020

Preprint

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Background: Staphylococcus aureus is an important cause of foodborne diseases due to the consumption of contaminated raw chicken meat. The present research was performed to evaluate the phenotypic and genotypic properties of antibiotic resistance, virulence factor profiles and molecular typing of S. aureus strains isolated from chicken meat. A total of 36 S. aureus strains were isolated from raw chicken meat samples. Phenotypic pattern of antibiotic resistance was assessed by disk diffusion. Distribution of antibiotic resistance and virulence genes was evaluated by PCR. Molecular typing of isolates was perform ed by the ERIC-PCR. Results:Considering the over than 80% similarity, 36 S. aureus isolates were classified in 9 different profiles with 43 to 100% similarities. S. aureus strains showed the highest incidence of resistance against penicillin (100%), tetracycline (91.66%), cephalothin (77.77%), ciprofloxacin (75%), erythromycin (75%), mupirocin (63.88%), clindamycin (61.11%) and trimethoprim/sulfamethoxazole (61.11%). The most commonly detected antibiotic resistance genes amid the S. aureus isolates were mecA (100%), tetK (80.55%), tetM (66.66%), aacA-D (61.11%), msrA (55.55%) and ermA (55.55%).Total distribution of etB, etA, tsst-1, clfA and coa virulence factors amongst the S. aureus strains was 61.11%, 58.33%, 13.88%, 75% and 100%, respectively. Conclusions:Genetic cluster of bacteria affected the antibiotic resistance and virulence characters of S. aureus strains. S. aureus strains with the same ERIC-genetic cluster had similar antibiotic resistance and virulence characters which may show their similar origins. Presence of one or more virulence factors and antibiotic resistance genes amongst the resistant-S. aureus strains signifies an important public health threat rendering the consumption of raw or undercooked chicken meat.

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“…There are only a few studies on S. aureus isolation from street vended foods from India and the studies carried out in Silchar city, Assam by Sharma and Mazumdar (2014) revealed that 14.2% street vended food samples were positive for S. aureus while in Gangtok and Nainital, it was detected in 19.5% and 33% street vended food samples (Kharel et al 2016). Various workers reported that between 2.42% and 69.74% restaurant/street food samples harbored MRSA (Rhee and Woo 2010;Rizek et al 2011;Ranjbar et al 2017), thus responsible for health implications to the consumer. In another study, enterotoxigenic S. aureus was detected in 91 (60%) samples of coriander sauce, 87 (58%) samples of coconut slices and 129 (86%) samples of readyto-eat salads in New Delhi and Patiala City (Ghosh et al 2007).…”

Section: Resultsmentioning

confidence: 99%

Virulent methicillin resistant Staphylococcus aureus (MRSA) in street vended foods

et al. 2019

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Street foods are one of the important sources of foodborne infections and Staphylococcus aureus is an important infectious agent transmitted through various sources including street foods. The methicillin-resistant S. aureus (MRSA) are of public health significance, hence the study was taken to assess the street foods as a source of MRSA, for which 430 street vended foods of animal origin (meat, milk, eggs and their products) and associated environmental samples were processed for isolation and characterization. A total of 52 (12.1%) S. aureus were isolated and resistant was observed to oxacillin (36.5%), cefoxitin (25%) and penicillin G (82.7%) by disc diffusion test. On genotypic screening, mecA and blaZ have detected in 17.3% and 69.2% isolates, respectively. The virulence typing identified nuc, coa, clfA, spA, FnbA and enterotoxin A (sea) genes in 100%, 96.2%, 30.8%, 55.8, 50% and 7.7% isolates, respectively. Genetic diversity among the isolates was observed by enterobacterial repetitive intergenic consensus PCR with a D value of 0.77. The presence of virulent MRSA in street vended foods trigger the public health concern and emphasis to educate the consumers and street food vendors about quality and safety of such foods.

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Methicillin-resistant <i>Staphylococcus aureus</i> isolates from Iranian restaurant food samples: Panton-Valentine Leukocidin, SCCmec phenotypes and antimicrobial resistance

Cited by 10 publications

References 19 publications

Antimicrobial Resistance Properties of Staphylococcus Aureus Isolates From Powdered Packaged Medicinal Plants and Bottle Herbal Distillates

Antimicrobial Resistance Properties of Staphylococcus Aureus Isolates From Powdered Packaged Medicinal Plants and Bottle Herbal Distillates

Molecular typing, phenotypic and genotypic assessment of antibiotic resistance and virulence factors amongst the Staphylococcus aureus bacteria isolated from raw chicken meat

Virulent methicillin resistant Staphylococcus aureus (MRSA) in street vended foods

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