Methionine analogues HMB and HMBi increase the abundance of cellulolytic bacterial representatives in the rumen of cattle with no direct effects on fibre degradation

Martin, Cécile; Mirande, Caroline; Morgavi, Diego; Forano, Evelyne; Devillard, Estelle; Mosoni, Pascale

doi:10.1016/j.anifeedsci.2013.03.008

Cited by 29 publications

(43 citation statements)

References 37 publications

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“…These feedstuffs were incubated in nylon bags that allowed monitoring at the same time colonization by microbial populations and consequences in terms of substrate degradation in the rumen. Fibrobacter succinogenes was found to colonize less the feedstuffs studied here than corn materials previously investigated in another study using the same quantification method but different qPCR primers (Martin et al 2013). However, it remained predominant on most of the substrates compared with R. flavefaciens and B. fibrisolvens, in agreement with other studies (Mosoni et al 2011;Saro et al 2012).…”

Section: Discussionmentioning

confidence: 56%

“…Fibrobacter succinogenes was found to colonize less the feedstuffs studied here than corn materials previously investigated in another study using the same quantification method but different qPCR primers (Martin et al . ). However, it remained predominant on most of the substrates compared with R. flavefaciens and B. fibrisolvens, in agreement with other studies (Mosoni et al .…”

Section: Discussionmentioning

confidence: 97%

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Live yeasts enhance fibre degradation in the cow rumen through an increase in plant substrate colonization by fibrolytic bacteria and fungi

et al. 2016

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Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 97%

Live yeasts enhance fibre degradation in the cow rumen through an increase in plant substrate colonization by fibrolytic bacteria and fungi

et al. 2016

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“…Seymour et al () showed content of milk fat had a positive association with rumen acetate‐to‐propionate ratio. Martin et al () reported that HMB supplementation increased VFA concentrations in the rumen as well as the ruminal abundance of F. succinogenes and R. flavefaciens . VFAs are the end products of rumen microbial fermentation and represent the main supply of metabolizable energy for ruminants, suggesting increased energy supply to support milk production or BW gain.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies indicated 40‐50% ruminal escape of HMB (Vázquez‐Añón et al ; Koenig et al ), which showed a significant amount of HMB was metabolized in the rumen. Because of the ruminal degradability, HMB could affect microbial fermentation by a direct stimulatory effect of Met on certain cellulolytic bacteria or through an indirect effect on the noncellulolytic species in the rumen (Martin et al ). The exact mode of action of HMB in the rumen is not clear, but may be probably related to the stimulatory effect on microbial growth, particularly of some cellulolytic bacteria.…”

Section: Discussionmentioning

confidence: 99%

Effects of addition of Aspergillus oryzae culture and 2‐hydroxyl‐4‐(methylthio) butanoic acid on milk performance and rumen fermentation of dairy cows

Sun

Wang³

et al. 2016

Animal Science Journal

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To investigate effects of Aspergillus oryzae culture (AOC) and 2-hydroxy-4-(methylthio) butanoic acid (HMB) on milk performance and rumen fermentation of dairy cows. Sixty-four multiparous Chinese Holstein cows were randomly allocated into four experimental diets: (i) Control diet; (ii) AOC diet: 5 g AOC/day per head; (iii) HMB diet: 25 g HMB/day; and (iv) AH diet: 5 g AOC plus 25 g HMB/day. Added HMB tended to increase the yield of milk protein (P = 0.06) and 3.5% fat-corrected milk (P = 0.08) and milk fat content (P = 0.09). Milk fat yield (P = 0.03) and the contents of milk protein (P = 0.05) were increased by adding HMB. The cows fed on AOC diet had a tendency for higher body weight (BW) gain (P = 0.08). Addition of AOC, HMB and AH increased content of microbial protein (MCP) and total volatile fatty acids (VFA) (P < 0.01) in rumen fluid. Populations of rumen fungi, Fibrobacter succinogenes and Ruminococcus flavefaciens relative to total bacterial 16S rDNA (P ≤ 0.03) and activity of carboxymethylcellulase (CMCase) (P < 0.01) were increased with added AOC or HMB. It is inferred that added AOC or HMB can increase the contents of MCP and total VFA potentially by stimulating rumen microbe populations and CMCase activity.

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“…Limited data have suggested a possible effect of HM-TBa on rumen fermentation, fiber degradability, and microbial protein synthesis. With a reported ruminal degradability of 50% or more (Koenig et al, 1999(Koenig et al, , 2002Noftsger et al, 2005), HMTBa could affect microbial fermentation by a direct stimulatory effect of Met on certain cellulolytic bacteria or through an indirect effect on the noncellulolytic species in the rumen (Martin et al, 2013). Vazquez-Añon et al (2001) reported, for example, a quadratic increase in bacterial N outflow and the efficiency of bacterial N synthesis in continuous culture fermenters.…”

Section: Introductionmentioning

confidence: 99%

Effect of 2-hydroxy-4-methylthio-butanoic acid on ruminal fermentation, bacterial distribution, digestibility, and performance of lactating dairy cows

Lee

Hristov

et al. 2015

Journal of Dairy Science

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The objective of this experiment was to test the effect of a Met analog, 2-hydroxy-4-methylthio-butanoic acid (HMTBa), on ruminal fermentation and microbial protein synthesis, nutrient digestibility, urinary N losses, and performance of dairy cows. Eight multiparous lactating Holstein dairy cows were assigned to 4 levels of HMTBa [0 (control), 0.05, 0.10, and 0.15% (dry matter basis)] in a replicated 4×4 Latin square trial. Experimental periods were 28 d, including 21 d for adaptation. Ruminal ammonia and microbial N were labeled through a 6-d intraruminal infusion of (15)NH4Cl, and microbial protein synthesis in the rumen was estimated using the reticular sampling technique. Treatment had no effect on dry matter intake (28.4 to 29.8kg/d), milk yield (44.1 to 45.3kg/d), feed efficiency, and milk composition. Total-tract apparent digestibility of nutrients was generally not affected by treatment, except digestibility of crude protein and starch decreased quadratically with HMTBa supplementation. Fecal, but not urinary, and total excreta N losses were increased quadratically by HMTBa. Ruminal pH, ammonia concentration, protozoal counts, and the major volatile fatty acids were not affected by treatment. Microbial N outflow from the rumen was linearly increased by HMTBa. 2-Hydroxy-4-methylthio-butanoic acid linearly increased the proportion of Fecalibacterium and quadratically decreased the proportion of Eubacterium in ruminal contents. Of the individual bacterial species, HMTBa increased or tended to increase Prevotella loescheii and Prevotella oralis. 2-Hydroxy-4-methylthio-butanoic acid linearly increased the concentration (and yield) of 15:0 in milk fat. In the conditions of this crossover experiment, HMTBa had no effect on feed intake and performance of dairy cows, decreased dietary crude protein digestibility, and increased microbial N outflow from the rumen.

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Methionine analogues HMB and HMBi increase the abundance of cellulolytic bacterial representatives in the rumen of cattle with no direct effects on fibre degradation

Cited by 29 publications

References 37 publications

Live yeasts enhance fibre degradation in the cow rumen through an increase in plant substrate colonization by fibrolytic bacteria and fungi

Live yeasts enhance fibre degradation in the cow rumen through an increase in plant substrate colonization by fibrolytic bacteria and fungi

Effects of addition of Aspergillus oryzae culture and 2‐hydroxyl‐4‐(methylthio) butanoic acid on milk performance and rumen fermentation of dairy cows

Effect of 2-hydroxy-4-methylthio-butanoic acid on ruminal fermentation, bacterial distribution, digestibility, and performance of lactating dairy cows

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