Methionine sulfoxide reductase B5 plays a key role in preserving seed vigor and longevity in rice (Oryza sativa)

Abstract

Summary Oxidation of methionine leads to the formation of methionine S‐sulfoxide and methionine R‐sulfoxide, which can be reverted by two types of methionine sulfoxide reductase (MSR): MSRA and MSRB. Though the role of MSR enzymes has been elucidated in various physiological processes, the regulation and role of MSR in seeds remains poorly understood. In this study, through molecular, biochemical, and genetic studies using seed‐specific overexpression and RNAi lines of OsMSRB5 in Oryza sativa, we demonstrate the role of OsMSRB5 in maintaining seed vigor and longevity. We show that an age‐induced reduction in the vigor and viability of seeds is correlated with reduced MSR activity and increased methionine sulfoxide (MetSO) formation. OsMSRB5 expression increases during seed maturation and is predominantly localized to the embryo. Further analyses on transgenic lines reveal the role of OsMSRB5 in modulating reactive oxygen species (ROS) homeostasis to preserve seed vigor and longevity. We show that ascorbate peroxidase and PROTEIN l‐ISOASPARTYL METHYLTRANSFERASE undergo MetSO modification in seeds that affects their functional competence. OsMSRB5 physically interacts with these proteins and reverts this modification to facilitate their functions and preserve seed vigor and longevity. Our results thus illustrate the role of OsMSRB5 in preserving seed vigor and longevity by modulating ROS homeostasis in seeds.

Arabidopsis AtMSRB5 functions as a salt-stress protector for both Arabidopsis and rice

“…Despite having high amino acid sequence similarity (83%), our study suggests that AtMSRB5 and OsMSRB5 are involved in different stress tolerance mechanisms. Hazra et al (2022) demonstrate that OsMSRB5 is involved in maintaining seed vigor and longevity. This finding also supports our prediction.…”

Methionine biosynthetic genes and methionine sulfoxide reductase A are required for Rhizoctonia solaniAG1‐IA to cause sheath blight disease in rice

“…The growth defects of buffer‐treated and Rs_MET13 ‐silenced R. solani on 10 mM H 2 O 2 plates were fully restored upon methionine, but not with methionine sulfoxide (MetSO, an oxidized form of methionine) supplementation (Figures 3A and S5 ). Considering that organisms utilize methionine sulfoxide reductases (Msr) to repair oxidized methionine (Hazra et al., 2022 ; Moskovitz & Smith, 2021 ), we explored whether R. solani utilizes Msr to manage oxidative stress during infection in rice.…”

“…It will be important to identify R. solani proteins that are subject to MetSO accumulation during oxidative stress and investigate Rs_MsrAmediated repair of such damage. We anticipate that similar to plants (Hazra et al, 2022), various antioxidant enzymes (such as catalase and ascorbate peroxidase) of R. solani may be prone to MetSO-accumulation under oxidative stress and Rs_MsrA-mediated repair of these enzymes may be important for causing disease. In recent years, the spray-induced gene silencing (SIGS) approach has gained impetus to control plant diseases (McRae et al, 2023;Mitter et al, 2017;Qiao et al, 2021;Wang & Jin, 2017) and we emphasize that a suitable formulation for dsRNA-mediated silencing of Rs_MsrA will be an effective approach to control sheath blight disease in rice.…”

The association of protein-bound methionine sulfoxide with proteomic basis for aging in beech seeds

“…The fact that these proteins were not identified in Table 1 might be related to the presence of 1891 “uncharacterized proteins” in the list of identified proteins (Table S1 ), and further types and isoforms of Msrs might be hidden under this term. Recently, MsrB5 was found to maintain vigor and longevity in rice seeds [ 78 ]. Protein A0A2N9F0L3, assigned as MsrB5, increased in abundance in aged beech seeds (Table 1 ).…”