Method Development and Validation of Forced Degradation Studies of Carvedilol by Using Uv Spectroscopy

Abstract

INTRODUCTION:3-(9H-carbazol-4-yloxy)-2-hydroxypropyl [2-(2methoxyphenoxy) ethyl] amine (Figure1) Carvedilol is used to treat heart failure and hypertension (high blood pressure). It is also used after a heart attack that has caused your heart not to pump as well. Mechanism of action Carvedilol is a racemic mixture in which nonselective beta-adrenoreceptor blocking activity is present in the S (-) enantiomer and alpha-adrenergic blocking activity is present in both R (+) and S (-) enantiomers at equal potency. Carvedilol's beta-adrenergic receptor blocking ability decreases the heart rate, myocardial contractility, and myocardial oxygen demand [1][2][3][4]. Carvedilol also decreases systemic vascular resistance via its alpha adrenergic receptor blocking properties. Carvedilol and its metabolite BM-910228 (a less potent beta blocker, but more potent antioxidant) have been shown to restore the inotropic responsiveness to Ca 2+ in OHfree radical-treated myocardium. Carvedilol and its metabolites also prevent OHradical-induced decrease in sarcoplasmic reticulum Ca 2+ -ATPase activity. Therefore, carvedilol and its metabolites may be beneficial in chronic heart failure by preventing free radical damage [4][5][6][7]. The main object of the work is to develop a new method development and validation of forced degradation Studies of carvedilol by using UV spectroscopy [7][8][9][10].