INTRODUCTION:3-(9H-carbazol-4-yloxy)-2-hydroxypropyl [2-(2methoxyphenoxy) ethyl] amine (Figure1) Carvedilol is used to treat heart failure and hypertension (high blood pressure). It is also used after a heart attack that has caused your heart not to pump as well. Mechanism of action Carvedilol is a racemic mixture in which nonselective beta-adrenoreceptor blocking activity is present in the S (-) enantiomer and alpha-adrenergic blocking activity is present in both R (+) and S (-) enantiomers at equal potency. Carvedilol's beta-adrenergic receptor blocking ability decreases the heart rate, myocardial contractility, and myocardial oxygen demand [1][2][3][4]. Carvedilol also decreases systemic vascular resistance via its alpha adrenergic receptor blocking properties. Carvedilol and its metabolite BM-910228 (a less potent beta blocker, but more potent antioxidant) have been shown to restore the inotropic responsiveness to Ca 2+ in OHfree radical-treated myocardium. Carvedilol and its metabolites also prevent OHradical-induced decrease in sarcoplasmic reticulum Ca 2+ -ATPase activity. Therefore, carvedilol and its metabolites may be beneficial in chronic heart failure by preventing free radical damage [4][5][6][7]. The main object of the work is to develop a new method development and validation of forced degradation Studies of carvedilol by using UV spectroscopy [7][8][9][10].

In the present study we are reporting  dissolution, method development and validation of water soluble vitamins B1, B2 & B6 in bulk and tablet dosage form by HPTLC method. The method is based on separation of the three vitamins using HPTLC. Thin layer chromatographic plates coated with silica gel 60F254 as the stationary phase and acetonitrile:water (6:4 v/v) as mobile phase. The chromatographic analysis was carried out in the reflectance and absorbance mode at 280 nm. The method was validated with respect to linearity, accuracy and precision, limit of detection and limit of quantitation. It was then applied for analysis of vitamins B1, B2 & B6 in combined tablet dosage form. The above method developed was reproducible with good resolution and the results of analysis have been validated with correlation coefficient of 0.9990

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Around 3500 laboratory-confirmed cases of monkeypox infection, from 42 non-endemic countries have been reported to World Health Organization (WHO), during the current multi-country monkeypox outbreak. As of now, patients with monkeypox infection are managed symptomatically with supportive care. Hence, our current review focuses on the analysis of the pharmacotherapeutic potentials of black seeds (N. sativa). The literature was searched in web-based databases including web of science, Medline/PMC/PubMed, Embase, EBSCO, Google Scholar, Science Direct, and reference lists to identify relevant publications. Several clinical, animal, in-vivo, in-vitro, and in-silico studies have confirmed the antiviral efficacy of N. sativa against various viruses. In addition, previous research have demonstrated the analgesic, anti-inflammatory, antibacterial, antioxidant, and immunomodulatory effects of Black seeds (N. sativa), which would help to alleviate the signs, symptoms, and complications associated with monkeypox infection. In consequence, black seeds (N. sativa) could be added as an adjuvant therapy along with repurposed/investigational antiviral drugs and supportive care in the management of patients with monkeypox infection in early stages to prevent inflammatory conditions and secondary bacterial infections. The safety and efficacy of Black seeds (N. sativa) in the management of monkeypox infection would further be established by future randomized controlled clinical trials.