Method development and validation procedure for the chromatographic assay of cocaine and its metabolites in pharmacokinetic studies

Tamisier-Karolak, L.; Tod, Michel; Petitjean, Olivier; Cardot, Philippe

doi:10.1007/bf02263895

Cited by 6 publications

(2 citation statements)

References 25 publications

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“…it is a pure peak) when the spectra in all of its points coincide with the spectrum for the compound concerned. EME is not studied because the detector does not allow its determination at wavelengths above 200 nm [17].…”

Section: Resultsmentioning

confidence: 99%

HPLC–DAD determination of opioids, cocaine and their metabolites in plasma

Fernández

Morales

Vázquez

et al. 2006

Forensic Science International

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Section: Resultsmentioning

confidence: 99%

HPLC–DAD determination of opioids, cocaine and their metabolites in plasma

Fernández

Morales

Vázquez

et al. 2006

Forensic Science International

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“…Although, this represents a decrease from the 5.7 million users in 1985, the abuse of this addictive stimulant has become a persistent problem in the USA. [4] Therefore, BE is often the analyte of choice for detecting COC use. In addition to its addictive properties, COC has been shown to be toxic to both the CNS and the cardiovascular system.…”

Section: Introductionmentioning

confidence: 99%

Rapid Determination of Selected Drugs of Abuse in Human Plasma Using a Monolithic Silica HPLC Column and Solid Phase Extraction

Caufield¹,

Stewart²

2002

Journal of Liquid Chromatography & Related Technologies

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Two new high performance liquid chromatography (HPLC) assays were developed, which utilized a 10064.6 mm I.D. monolithic silica column and binary mobile phase gradients, for the simultaneous determination of selected drugs of abuse in human plasma. Both methods used gradients consisting of 25 mM pentanesulfonic acid and 25 mM sodium phosphate monobasic monohydrate-acetonitrile (pH 2.9), and mixed mode solid phase extraction procedures. In the first method, cocaine (COC) and its metabolites benzoylecgonine (BE), norcocaine (NC), and cocaethylene (CE), were separated with a pump flow rate of 5.0 mL=min in a total run time of 5 min. All analyses were conducted at ambient temperature. The injection volume was 100 mL and the UV detector was operated at 231 nm. The method was validated over the range of 50-5000 ng=mL for BE, COC, and CE, and 25-2500 ng=mL for NC. The method proved to be accurate (% bias for all calibration samples varied from À4.5 to 8.5%) and precise (within-run precision ranged from 1.5 to 12.8% and between-run precision ranged from 0.4 to 12.7%). The mean absolute recoveries were 93.5, 95.7, 105.4, and 98.8% for BE, COC, NC, and CE, respectively.In the second method, morphine (MO), hydromorphone (HM), tolazoline (ISTD), codeine (CO), oxycodone (OC), and hydrocodone (HC) were separated with a pump flow rate of 8.0 mL=min in a total run time of 2 min. All analyses were conducted at 30 C temperature. The injection volume was 100 mL and the UV detector was operated at 208 nm. The method was validated over the range of 50-5000 ng=mL for the opiates studied. The method proved to be accurate (% bias for all calibration samples varied from À8.4 to 2.0%) and precise (within-run precision ranged from 1.7 to 16.9% and between-run precision ranged from 0.4 to 14.8%). The mean absolute recoveries were 95.6, 104, 103, 97.9, and 105% for MO, HM, CO, OC, and HC, respectively. The recovery for the internal standard was 99.6%. The assays should be suitable for use in routine determination of the selected drugs of abuse in human plasma.

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