Method Development for the Determination of Free and Esterified Sterols in Button Mushrooms (Agaricus bisporus)

Abstract

Ergosterol is the major sterol in button mushrooms (Agaricus bisporus) and can occur as free alcohol or esterified with fatty acids (ergosteryl esters). In this study, gas chromatography with mass spectrometry in the selected ion monitoring mode (GC/MS-SIM) was used to determine ergosterol and ergosteryl esters as well as other sterols and steryl esters in button mushrooms. Different quality control measures were established and sample preparation procedures were compared to prevent the formation of artifacts and the degradation of ergosteryl esters. The final method was then used for the determination of ergosterol (443 ± 44 mg/100 g dry matter (d.m.)) and esterified ergosterol (12 ± 6 mg/100 g d.m.) in button mushroom samples (n = 4). While the free sterol fraction was vastly dominated by ergosterol (∼90% of five sterols in total), the steryl ester fraction was more diversified (nine sterols in total, ergosterol ∼55%) and consisted primarily of linoleic acid esters.

Countercurrent chromatographic isolation and purification of 11′-α-tocomonoenol from the vitamin E extract of palm oil

“…An aliquot of the crude extract (section 2.3) was measured on a 6890/5973 GC/MS system (Agilent Technologies, Santa Clara, CA, USA), equipped with a 30 m HP-5MS column (0.25 mm internal diameter, 0.25 μm film thickness) as recently described in detail [19]. The temperature program started at 55 °C (1 min), was raised with 20 °C/min to 255 °C by 1.5…”

“…°C/min to 283 °C and finally by 15 °C/min to 300 °C which was held for 5 min (total run time 35.8 min) [19]. All other GC measurements were performed with a second 6890/5973 GC/MS system equipped with a cool on-column inlet (Hewlett-Packard/Agilent, Waldbronn, Germany), a pre-column (2 m, 0.53 mm i.d., deactivated with 1,3-diphenyl-1,1,3,3tetramethyldisilazane; BGB Analytics, Böckten, Switzerland) and a 15 m, 0.25 mm i.d.…”

Chemical Screening of Metabolites Profile from Romanian Tuber spp.

“…The steroid compounds found in truffles are involved in the mechanism of tumor protection and angiogenesis [7,23,26,[46][47][48][49][50]. Furthermore, truffles contain stigmasterol and beta-sitosterol, compounds with similar chemical structures to cholesterol.…”

Nodular panniculitis in a cat with high alpha tocopherol concentration in serum

“…The solvent was again removed using nitrogen and taken up in a 5α‐cholestane solution (1 ml; 4 µg/ml, w/v, dissolved in n ‐hexane). GC‐MS analysis was performed via a 6890/5973N GC‐MS system (Agilent) equipped with a HP5‐MS column (30 m × 0.25 mm i.d., 0.25 µm film thickness; Agilent) as described by Hammann and Vetter (2016). The following temperature programme was used: 60°C, raised at 20°C/min to 255°C, at 1.5°C/min to 283°C and finally at 15°C/min to 300°C (held for 5 min), leading to a total run time of 35.6 min.…”