Method development in liquid chromatography with a charged cyclodextrin additive for chiral resolution ofrac-amlodipine utilising a central composite design

A simple and rapid method for chiral separation of amlodipine using dual chiral mobile phase additives

“…34,35 In addition, the use of chiral mobile phase additives has been explored to separate the racemates of amlopidine. 36 The technique of CMPAs has several advantages compared to CSPs. For instance, it employs achiral stationary phases, which are less expensive and more rugged than a chiral stationary phase.…”

“…37 The Fell group, in particular, has produced the earliest example of the resolution of rac-amlodipine using a charged cyclodextrin additive in 52 min. 36 Soon aer, they have shown that amlodipine enantiomers could be separated within 23 min on a C8 column using 20 mmol L À1 SBE-b-CD. 33 In 2010, Chen et al demonstrated that the enantiomers of amlodipine besylate could be separated within 34 min using SBE-b-CD as the additive.…”

Optimization and validation of a new CE method for the determination of pantoprazole enantiomers

“…In addition, chiral HPLC requires a large volume of organic solvents. The negatively charged sulfobutylether‐beta‐cyclodextrin (SBE‐beta‐CD) is one of the popular beta‐CD derivatives and has been applied successfully as chiral selector in both CE [] and HPLC [] because of a dramatically improved aqueous solubility. The sulfonic groups provide a derivative, which is anionic over the entire pH range accessible to CE experiments and which can thereby lead to ionic interaction in addition to the hydrophobic inclusion.…”

Determination of the enantiomer and positional isomer impurities in atomoxetine hydrochloride with liquid chromatography using polysaccharide chiral stationary phases

“…Normal or reversedphase LC separations can be developed by evaluating solvent systems, chromatographic columns, aqueous buffer systems where appropriate, mobile phase pH, and/or temperature. Another option is to examine a more orthogonal chromatographic approach [16,17] which may include chiral chromatography either in reversed or normal-phase mode and using either a chiral mobile phase additive (CMPA) [18] or a CSP [19][20][21][22][23]. Furthermore, an orthogonal analytical technique may also be utilized and positional isomer separations have been reported using capillary electrophoresis (CE) [19,24], capillary electrochromatography [25], supercritical fluid chromatography [26] and gas chromatography [27].…”