Method Development with Solid Phase Microextraction

Penton, Zelda E.

doi:10.1201/9781439832387.ch2

Cited by 9 publications

(6 citation statements)

References 10 publications

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“…Response Factors and Standard Curve. The use of standard addition curves to quantitate is often recommended for SPME procedures (15). If the purpose of a study is to analyze for differences among a variety of samples, standard addition curves need to be constructed within each sample, which may be a cumbersome process.…”

Section: Resultsmentioning

confidence: 99%

Analysis of Free Fatty Acids in Whey Products by Solid-Phase Microextraction

Tomaino

Parker

Larick

2001

J. Agric. Food Chem.

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To evaluate the impact of Cheddar cheese starter cultures on the level of free fatty acids in liquid whey, a solid-phase microextraction (SPME) technique was utilized. The determination of response factors relative to an internal standard and the verification of linearity over a wide concentration range allowed for the quantitation of free fatty acids in experimentally produced liquid whey and in a wide range of dry whey ingredients. Results indicated that whey produced with a Lactococcus lactis subsp. lactis starter culture contained the highest level of total free fatty acids with significantly higher levels of lauric, myristic, and palmitic acids. Significant declines in oleic, linoleic, and palmitic acid occurred during storage. Commercial whey ingredients demonstrated a linear increase in free fatty acids with increasing percent protein, except for whey protein isolate, which had the lowest concentration. The use of SPME for the rapid analysis of free fatty acids in whey products should allow for further research to determine the importance of these compounds on the quality and stability of whey products.

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Section: Resultsmentioning

confidence: 99%

Analysis of Free Fatty Acids in Whey Products by Solid-Phase Microextraction

Tomaino

Parker

Larick

2001

J. Agric. Food Chem.

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“…Electrolytes, such as salt, in an aqueous system can influence the phase boundary properties and decrease the solubility of hydrophobic compounds by competing water molecules, which is called "salting out" (19,20). Although the efficiency of volatile compound extraction depends on the concentration of salt solution, a 20-30% (w/v) salt concentration was reported to be sufficient to give the best sensitivity for most volatile compounds (20).…”

Section: Effects Of Sample Preparation Temperature and Samplementioning

confidence: 99%

Optimization of Solid Phase Microextraction Analysis for the Headspace Volatile Compounds of Parmesan Cheese

Lee

Diono

Kim

et al. 2003

J. Agric. Food Chem.

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Optimum conditions of solid phase microextraction (SPME) analysis of the headspace volatile compounds of Parmesan cheese in airtightly sealed 100-mL bottles were developed. The coefficient of variation of SPME analysis on the headspace volatile compounds of Parmesan cheese was 2%. The reproducibility of SPME was improved by a combination of sampling at -10 degrees C, controlling the sample temperature, and uniform magnetic stirring of samples during equilibrium and isolation steps. The sensitivity of SPME increased by 125% in total peak areas by a combination of 40 min of sonication and 25% (w/v) sodium phosphate solution, compared with that of samples containing deionized water only (P < 0.05). The addition of salt solution or sonication treatment in samples increased the headspace volatile compounds of cheese quantitatively without producing any new volatile compounds.

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“…These techniques comprise a simple, efficient and solventless extraction method that is suitable for trace analysis. However, SPME has some drawbacks, such as relatively high cost of fibers and limited lifetime of fibers associated with pollution during the extraction and eventual degradation (Penton, 1999). Liquidphase microextraction, an alternative solvent-miniaturized extraction method, has been developed to overcome the problems of SPME, and it is a simple, fast and cost-effective sample preparation technique.…”

Section: Introductionmentioning

confidence: 99%

Solvent bar microextraction combined with high‐performance liquid chromatography for preconcentration and determination of pramipexole in biological samples

Ghasemi

Kheradmand

Dadrass

2013

Biomedical Chromatography

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A simple, rapid and sensitive analytical method for preconcentration and determination of pramipexole in different biological samples has been developed using solvent bar microextraction (SBME) combined with HPLC-UV. The target drugs were extracted from 10 mL of basic aqueous sample solution into an organic extracting solvent located inside the pores of a polypropylene hollow fiber, then back-extracted into an acidified aqueous solution in the lumen of the hollow fiber. In order to obtain high extraction efficiency, the effect of different variables on the extraction efficiency was studied simultaneously using an experimental design. The experimental parameters of SBME were optimized using a Box-Behnken design after a Plackett-Burman screening design. Under the optimized conditions, an enrichment factor up to 96 was achieved and the relative standard deviation of the method was 4.64% (n = 5). The linear range was 0.05-2000 µg/L with a correlation coefficient (r) of 0.987. Finally, the applicability of the proposed method was evaluated by extraction and determination of pramipexole in plasma and urine samples. The results indicated that SBME method has excellent clean-up and high preconcentration factor and can serve as a simple and sensitive method for analysis of pramipexole in biological samples.

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Method Development with Solid Phase Microextraction

Cited by 9 publications

References 10 publications

Analysis of Free Fatty Acids in Whey Products by Solid-Phase Microextraction

Analysis of Free Fatty Acids in Whey Products by Solid-Phase Microextraction

Optimization of Solid Phase Microextraction Analysis for the Headspace Volatile Compounds of Parmesan Cheese

Solvent bar microextraction combined with high‐performance liquid chromatography for preconcentration and determination of pramipexole in biological samples

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